Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro

Abstract

Abstract: Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides.The optimal medium for IPS production consists of glucose 54.50 g·L^-1,yeast powder 25.50 g·L^-1,NaH₂PO₄ 0.4 g·L^-1 and K₂HPO₄ 0.4 g·L^-1.The suggested culture conditions are 24 ℃,initial pH 4.5 with a rotary speed of 120 r·min^-1 for 168 h.The yield of IPS is 737.93 mg·L^-1,which is 50% higher than the yield under the conditions prior to optimization.The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay.The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms.

Key words: antioxidant activity, Cordyceps ophioglossoides L2, intracellular polysaccharide, submerged culture, optimization

摘要： Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides.The optimal medium for IPS production consists of glucose 54.50 g·L^-1,yeast powder 25.50 g·L^-1,NaH₂PO₄ 0.4 g·L^-1 and K₂HPO₄ 0.4 g·L^-1.The suggested culture conditions are 24 ℃,initial pH 4.5 with a rotary speed of 120 r·min^-1 for 168 h.The yield of IPS is 737.93 mg·L^-1,which is 50% higher than the yield under the conditions prior to optimization.The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay.The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms.

关键词: antioxidant activity, Cordyceps ophioglossoides L2, intracellular polysaccharide, submerged culture, optimization

XU Qinqin, LIU Zhenhua, SUN Yisheng, DING Zhongjie, Lü Longxian, LI Yongquan. Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro[J]. Chin.J.Chem.Eng., 2012, 20(2): 294-301.

许勤勤, 刘振华, 孙一晟, 丁忠杰, 吕龙贤, 李永泉. Optimization for Production of Intracellular Polysaccharide from Cordyceps ophioglossoides L2 in Submerged Culture and Its Antioxidant Activities in vitro[J]. Chinese Journal of Chemical Engineering, 2012, 20(2): 294-301.

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