One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization*

Chin.J.Chem.Eng. ›› 2012, Vol. 20 ›› Issue (5): 923-929.

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*

Sadia Ilyas^1,2, CHI Ru'an¹, Jae Chun Lee², Haq Nawaz Bhatti³

1. Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering & Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China;
2. Mineral Resoures Research Division, Korea Institute of Geoscience and Mineral Resources (KIGAM), 124 Gwahang-no, Yuseong-gu, Daejeon 305-350, Korea;
3. Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan

Received:2011-03-22 Revised:2011-10-16 Online:2012-11-06 Published:2012-10-28
Supported by:
Supported by the Program for Changjiang Scholars and Innovative Research Team in University, China (IRT0974);the Higher Education Commission of Pakistan (20-652/R&D/05-43622)

One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*

Sadia Ilyas^1,2, 池汝安¹, Jae Chun Lee², Haq Nawaz Bhatti³

1. Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering & Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China;
2. Mineral Resoures Research Division, Korea Institute of Geoscience and Mineral Resources (KIGAM), 124 Gwahang-no, Yuseong-gu, Daejeon 305-350, Korea;
3. Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040, Pakistan

通讯作者: CHI Ru'an,E-mail:rac@mail.wit.edu.cn
基金资助:
Supported by the Program for Changjiang Scholars and Innovative Research Team in University, China (IRT0974);the Higher Education Commission of Pakistan (20-652/R&D/05-43622)

Abstract

Abstract: A study was carried out to examine the possibility for Aspergillus niger strain KBS4 to bioleach metals from sulphide ore with low concentration of arsenic and to optimize the parameters that affect this process by orthogonal array optimization. Fungal sample was collected, purified and sequenced. The bioleaching process was optimized with L25 Taguchi orthogonal experimental array design. Five factors were investigated and 25 batch bioleaching tests were run at five levels for each factor. The parameters were initial pH, particle size, pulp density, initial inoculums and residence time for bioleaching. The experimental results showed that under optimized leaching conditions: pH 5.5, particle size 180 μm, initial inoculums size 3×10⁷ spores per ml, pulp density 15% and residence time of 20 days, the bioleach ability of metals were 63% Fe, 68% Zn, 60% As, 79% Cu and 54% Al. The biosorption of metal ions by fungal biomass might occur during the bioleaching process but it did not hinder the removal of metal ions by bioleaching.

Key words: Aspergillus niger, bioleaching, sulphide ore, orthogonal array

摘要： A study was carried out to examine the possibility for Aspergillus niger strain KBS4 to bioleach metals from sulphide ore with low concentration of arsenic and to optimize the parameters that affect this process by orthogonal array optimization. Fungal sample was collected, purified and sequenced. The bioleaching process was optimized with L25 Taguchi orthogonal experimental array design. Five factors were investigated and 25 batch bioleaching tests were run at five levels for each factor. The parameters were initial pH, particle size, pulp density, initial inoculums and residence time for bioleaching. The experimental results showed that under optimized leaching conditions: pH 5.5, particle size 180 μm, initial inoculums size 3×10⁷ spores per ml, pulp density 15% and residence time of 20 days, the bioleach ability of metals were 63% Fe, 68% Zn, 60% As, 79% Cu and 54% Al. The biosorption of metal ions by fungal biomass might occur during the bioleaching process but it did not hinder the removal of metal ions by bioleaching.

关键词: Aspergillus niger, bioleaching, sulphide ore, orthogonal array

Sadia Ilyas, CHI Ru'an, Jae Chun Lee, Haq Nawaz Bhatti. One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*[J]. Chin.J.Chem.Eng., 2012, 20(5): 923-929.

Sadia Ilyas, 池汝安, Jae Chun Lee, Haq Nawaz Bhatti. One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 923-929.

References

1 Mulligan,C.N.,Galvez-Cloutier,R.,"Bioleaching of copper mining residues by Aspergillus niger",Water Sci.Technol.,41,255-262 (2000).
2 Valix,M.,Usai,F.,Malik,R.,"Fungal bioleaching of low-grade laterite ores",Min.Eng.,14,197-203 (2001).
3 Murr,L.E.,Berry,V.K.,"Direct observations of selective attachment of bacteria on low grade sulfide ores and other mineral surfaces",Hydrometallurgy J.,2,11-24 (1976).
4 Sukla,L.B.,Panchanadikar,V.V.,Kar,R.N.,"Microbial leaching of lateritic nickel ore",World J.of Microbiol.and Biotech.,9,255-257 (1993).
5 Krebs,W.,Brombacher,C.,Bosshard,P.P.,Bachofen,R.,Brandl,H.,"Microbial recovery of metals from solids",FEMS Microbiol.Rev.,20,605-617 (1997).
6 Bosecker,K.,"Bioleaching: metal solubilization by microorganisms",FEMS Microb.Rev.,20,591-604 (1997).
7 Wenberg,G.M.,Erbisch,F.H.,Volin,M.E.,"Leaching of copper by fungi",Mining Eng.,23,207-212 (1971).
8 Tzeferis,P.G.,Agatzini,S.,Nerantzis,E.T.,"Mineral leaching of non-sulphide nickel ores using heterotrophic micro-organisms",Lett.Appl.Microbiol.,18,209-213 (1994).
9 Bosshard,P.B.,Bachofen,R.,Brandl,H.,"Metal leaching of fly ash from municipal waste incineration by Aspergillus niger",Environ.Sci.Technol.,30,3066-3070 (1996).
10 Tasa,A.,Vourinen,A.,Garcia,O.,Tuovinen,O.,"Biologically enhanced dissolution of pyrite rich black shale concentrate",J.Environ.Sci.Health,32,2683-2695 (1997).
11 opur,M.,zmetin,C.,zmetin,E.,Kocakerim,M.M.,"Optimization study of the leaching of roasted zinc sulphide concentrate with sulphuric acid solutions",Chem.Eng.Process.,43,1007-1014 (2003).
12 Wei,J.C.,Handbook of Fungal Identification,Shanghai Scientific and Technical Publisher,Shanghai (1979).
13 White,T.J.,Bruns,T.,Lee,S.,Talor,J.,"Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics",PCR Protocols: A Guide to Methods and Applications,In Innis,M.A.,Gelfand,D.H.,Sninsky,J.J.,White T.J.,Eds.,Academic,San Diego,315-322 (1990).
14 Thompson,J.D.,Gibson,T.J.,Plewniak,F.,Jeanmougin,F.,Higgins,D.G.,"The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools",Nucleic Acids Research,24,4876-4882 (1997).
15 Tamura,K.,Dudley,J.,Nei,M.,Kumar,S.,"Molecular evolutionary genetics analysis (MEGA) software version 4.0",Molecular Biology and Evolution,24,1596-1599 (2007).
16 Safarzadeh,M.S.,Moradkhani,D.,Ilkhchi,M.O.,Golshan,N.H.,"Determination of the optimum conditions for the leaching of Cd-Ni residues from electrolytic zinc plant using statistical design of experiments",Sep.Purif.Technol.,58,367-376 (2008).
17 Xu,T.J.,Ting,Y.P.,"Fungal bioleaching of incineration fly ash: metal extraction and modeling growth kinetics",Enzyme Microb.Technol.,44,323-328 (2009).
18 Sayer,J.A.,Gadd,G.M.,"Binding of cobalt and zinc by organic acids and culture filtrates of Aspergillus niger grown in the absence or presence of insoluble cobalt or zinc phosphate",Mycol.Res.,105,1261-1267 (2001).
19 Mulligan,C.N.,Kamali,M.,Gibbs,B.F.,"Bioleaching of heavy metals from low-grade mining ore using Aspergillus niger",J.Hazard.Mater.,110,77-84 (2004).
20 Elliott,H.A.,Shastri,N.L.,"Extractive decontamination of metal-polluted soils using oxalate",Water,Air,Soil Pollut.,110,335-346 (1999).
21 Karaffa,L.,Kubicek,C.P.,"Aspergillus niger citric acid accumulation: do we understand this well working black box?",Appl.Microbiol.Biotechnol.,61,189 (2003).
22 Wu,H.Y.,Ting,Y.P.,"Metal extraction from municipal solid waste (MSW) incinerator fly ash-chemical leaching and fungal bioleaching",Enzyme Microb.Technol.,38,839 (2006).
23 Yang,J.,Wang,Q.H.,Wang,Q.,Wu,T.J.,"Comparisons of one-step and two-step bioleaching for heavy metals removed from municipal solid waste incineration fly ash",Environ.Eng.Sci.,5,783-789 (2008).
24 Castro,I.M.,Fietto,J.L.R.,Vieira,R.X.,Trópia,M.J.M.,Campos,L.M.M.,Paniago,E.B.,Branda,O,R.L.,"Bioleaching of zinc and nickel from silicates using Aspergillus niger cultures",Hydrometallurgy,57,39-49 (2000).
25 Aung,K.M.M.,Ting,Y.P.,"Bioleaching of spent fluid catalytic cracking catalyst using Aspergillus niger",J.Biotechnol.,116,159-170 (2005).
26 Schinner,F.,Burgstaller,W.,"Extraction of zinc from industrial waste by a Penicillium sp.",Appl.Environ.Microbiol.,55,1153-1156 (1989).
27 Yang,J.,Wang,Q.,Luo,Q.,Wang,Q.,Wu,T.,"Biosorption behavior of heavy metals in bioleaching process of MSWI fly ash by Aspergillus niger",Biochem.Eng.J.,46,294-299 (2009).
28 Tang,J.,Valix,M.,"Leaching of low-grade nickel ores by fungi metabolic acids",In: ECI Conference on Separations Technology Ⅵ: New Perspectives on Very Large-Scale Operations,Kingfisher Resort,Fraser Island,Queensland,Australia (2006).

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One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*

One Step Bioleaching of Sulphide Ore with Low Concentration of Arsenic by Aspergillus niger and Taguchi Orthogonal Array Optimization^*

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