Acarbose Isolation with Gel Type Strong Acid Cation Exchange Resin：Equilibrium, Kinetic and Thermodynamic Studies

doi:10.1016/S1004-9541(13)60583-2

Chin.J.Chem.Eng. ›› 2013, Vol. 21 ›› Issue (10): 1106-1113.DOI: 10.1016/S1004-9541(13)60583-2

• SEPARATION SCIENCE AND ENGINEERING • Previous Articles Next Articles

Acarbose Isolation with Gel Type Strong Acid Cation Exchange Resin：Equilibrium, Kinetic and Thermodynamic Studies

WANG Yajun^1,2, YU Lei^1,2, ZHENG Yuguo^1,2, WANG Yuanshan^1,2, SHEN Yinchu^1,2

1 Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China;
2 Engineering Research Center of Bioconversion and Biopurification, Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, China

Received:2012-04-22 Revised:2012-09-20 Online:2013-10-29 Published:2013-10-28
Contact: ZHENG Yuguo
Supported by:
Supported by the National Basic Research Program of China (2011CB710800) and National Special Program for Key Science and Technology of China (2008ZX09204-004).

Acarbose Isolation with Gel Type Strong Acid Cation Exchange Resin：Equilibrium, Kinetic and Thermodynamic Studies

王亚军^1,2, 于蕾^1,2, 郑裕国^1,2, 王远山^1,2, 沈寅初^1,2

1 Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China;
2 Engineering Research Center of Bioconversion and Biopurification, Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, China

通讯作者: ZHENG Yuguo
基金资助:
Supported by the National Basic Research Program of China (2011CB710800) and National Special Program for Key Science and Technology of China (2008ZX09204-004).

Abstract

Abstract: Acarbose, a potent α-glucosidase inhibitor, is widely used as an oral anti-diabetic drug for the treatment of the type 2, non-insulin-dependent diabetes. In this work, a gel type strong acid cation exchange resin 001×4 was applied to isolate acarbose from fermentation broth. It was demonstrated that cation exchanger 001×4 displayed a large adsorption capacity and quick exchange rate for acarbose. The static adsorption equilibrium data were well fitted to the Langmuir equation. Column adsorption experiments demonstrated that high dynamic adsorption capacity was reached at bed height of 104.4 mm, feed flow rate of 1.0 ml·min^-1 and acarbose concentration of 4.0 mg·ml^-1. Under the optimized conditions, the column chromatography packed with cation exchanger 001×4 recovered 74.3% (by mass) of acarbose from Actinoplanes utahensis ZJB-08196 fermentation broth with purity of 80.1% (by mass), demonstrating great potential in the practical applications in acarbose separation.

Key words: acarbose, separation, cation exchange, chromatography

摘要： Acarbose, a potent α-glucosidase inhibitor, is widely used as an oral anti-diabetic drug for the treatment of the type 2, non-insulin-dependent diabetes. In this work, a gel type strong acid cation exchange resin 001×4 was applied to isolate acarbose from fermentation broth. It was demonstrated that cation exchanger 001×4 displayed a large adsorption capacity and quick exchange rate for acarbose. The static adsorption equilibrium data were well fitted to the Langmuir equation. Column adsorption experiments demonstrated that high dynamic adsorption capacity was reached at bed height of 104.4 mm, feed flow rate of 1.0 ml·min^-1 and acarbose concentration of 4.0 mg·ml^-1. Under the optimized conditions, the column chromatography packed with cation exchanger 001×4 recovered 74.3% (by mass) of acarbose from Actinoplanes utahensis ZJB-08196 fermentation broth with purity of 80.1% (by mass), demonstrating great potential in the practical applications in acarbose separation.

关键词: acarbose, separation, cation exchange, chromatography

WANG Yajun, YU Lei, ZHENG Yuguo, WANG Yuanshan, SHEN Yinchu. Acarbose Isolation with Gel Type Strong Acid Cation Exchange Resin：Equilibrium, Kinetic and Thermodynamic Studies[J]. Chin.J.Chem.Eng., 2013, 21(10): 1106-1113.

王亚军, 于蕾, 郑裕国, 王远山, 沈寅初. Acarbose Isolation with Gel Type Strong Acid Cation Exchange Resin：Equilibrium, Kinetic and Thermodynamic Studies[J]. Chinese Journal of Chemical Engineering, 2013, 21(10): 1106-1113.

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Acarbose Isolation with Gel Type Strong Acid Cation Exchange Resin：Equilibrium, Kinetic and Thermodynamic Studies

Acarbose Isolation with Gel Type Strong Acid Cation Exchange Resin：Equilibrium, Kinetic and Thermodynamic Studies

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