SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2019, Vol. 27 ›› Issue (7): 1659-1665.DOI: 10.1016/j.cjche.2018.11.013

• Biotechnology and Bioengineering • Previous Articles     Next Articles

Increased sedimentation of a Pseudomonas-Saccharomyces microbial consortium producing medium chain length polyhydroxyalkanoates

Chang Liu1, Lin Qi1, Songyuan Yang1, Yun He1, Xiaoqiang Jia1,2,3   

  1. 1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
    2 Key Laboratory of Systems Bioengineering(Tianjin University), Ministry of Education, Tianjin 300072, China;
    3 SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China
  • Received:2018-08-03 Online:2019-10-14 Published:2019-07-28
  • Contact: Xiaoqiang Jia

Increased sedimentation of a Pseudomonas-Saccharomyces microbial consortium producing medium chain length polyhydroxyalkanoates

Chang Liu1, Lin Qi1, Songyuan Yang1, Yun He1, Xiaoqiang Jia1,2,3   

  1. 1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
    2 Key Laboratory of Systems Bioengineering(Tianjin University), Ministry of Education, Tianjin 300072, China;
    3 SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China
  • 通讯作者: Xiaoqiang Jia

Abstract: Concerns about feasibility, separability, settleability, efficiency once hampered studies on polyhydroxyalkanoates (PHAs) production, which mainly focused on single strain microorganism or activated sludge rather than artificial microbial consortia. Here, a medium chain length PHAs (mcl-PHAs) producing Pseudomonas-Saccharomyces consortium with xylose as the main substrate was studied. Mcl-PHAs accumulation increased from 12.69 mg·L-1 to 152.3 mg·L-1 without any optimization method. The presence of Saccharomyces cerevisiae, though in a relatively low concentration, improved the sedimentation of cell mass of the mixed culture by 60%. Reasons for better sedimentation of the consortium were complex:first, the length of Pseudomonas putida increased two to three times in the consortium; second, the positive surface charge of P. putida was neutralized by S. cerevisiae; third, the adhesion proteins on the surface of S. cerevisiae interacted with the P. putida.

Key words: Medium chain length, polyhydroxyalkanoates, Pseudomonas-Saccharomyces consortium, Sedimentation, Xylose

摘要: Concerns about feasibility, separability, settleability, efficiency once hampered studies on polyhydroxyalkanoates (PHAs) production, which mainly focused on single strain microorganism or activated sludge rather than artificial microbial consortia. Here, a medium chain length PHAs (mcl-PHAs) producing Pseudomonas-Saccharomyces consortium with xylose as the main substrate was studied. Mcl-PHAs accumulation increased from 12.69 mg·L-1 to 152.3 mg·L-1 without any optimization method. The presence of Saccharomyces cerevisiae, though in a relatively low concentration, improved the sedimentation of cell mass of the mixed culture by 60%. Reasons for better sedimentation of the consortium were complex:first, the length of Pseudomonas putida increased two to three times in the consortium; second, the positive surface charge of P. putida was neutralized by S. cerevisiae; third, the adhesion proteins on the surface of S. cerevisiae interacted with the P. putida.

关键词: Medium chain length, polyhydroxyalkanoates, Pseudomonas-Saccharomyces consortium, Sedimentation, Xylose