The Effect of pH Control on Acetone–Butanol–Ethanol Fermentation by Clostridium acetobutylicum ATCC 824 with Xylose and D-Glucose and D-Xylose Mixture

doi:10.1016/j.cjche.2014.06.003

Chin.J.Chem.Eng. ›› 2014, Vol. 22 ›› Issue (8): 937-942.DOI: 10.1016/j.cjche.2014.06.003

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

The Effect of pH Control on Acetone–Butanol–Ethanol Fermentation by Clostridium acetobutylicum ATCC 824 with Xylose and D-Glucose and D-Xylose Mixture

Wei Jiang¹, Zhiqiang Wen¹, Mianbin Wu^1,2, Hong Li³, Jun Yang¹, Jianping Lin¹, Yijun Lin¹, Lirong Yang¹, Peilin Cen¹

1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2. Zhejiang Key Laboratory of Antifungal Drugs, Zhejiang, Taizhou 318000, China;
3. Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK

Received:2013-04-11 Revised:2013-12-05 Online:2014-11-04 Published:2014-08-28
Supported by:
Supported by the National Natural Science Foundation of China (20306026 and 21376215) and the National High Technology Research and Development Program of China (2012AA022302).

The Effect of pH Control on Acetone–Butanol–Ethanol Fermentation by Clostridium acetobutylicum ATCC 824 with Xylose and D-Glucose and D-Xylose Mixture

Wei Jiang¹, Zhiqiang Wen¹, Mianbin Wu^1,2, Hong Li³, Jun Yang¹, Jianping Lin¹, Yijun Lin¹, Lirong Yang¹, Peilin Cen¹

1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2. Zhejiang Key Laboratory of Antifungal Drugs, Zhejiang, Taizhou 318000, China;
3. Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK

通讯作者: Mianbin Wu, Jianping Lin
基金资助:
Supported by the National Natural Science Foundation of China (20306026 and 21376215) and the National High Technology Research and Development Program of China (2012AA022302).

Abstract

Abstract: D-Glucose, L-arabinose, D-mannose, D-xylose, and cellobiose are saccharification products of lignocellulose and important carbon sources for industrial fermentation. The fermentation efficiency with each of the five sugars and the mixture of the two most dominant sugars, D-glucose and D-xylose, was evaluated for acetone- butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum ATCC 824. The utilization efficacy of the five reducing sugars was in the order of D-glucose, L-arabinose, D-mannose, D-xylose and cellobiose. D-Xylose, the second most abundant component in lignocellulosic hydrolysate, was used in the fermentation either as sole carbon source or mixed with glucose. The results indicated that maintaining pH at 4.8, the optimal pH value for solventogenesis, could increase D-xylose consumption when it was the sole carbon source. Different media containing D-glucose and D-xylose at different ratios (1:2, 1:5, 1.5:1, 2:1) were then attempted for the ABE fermentation. When pH was at 4.8 and xylose concentration was five times that of glucose, a 256.9% increase in xylose utilization and 263.7% increase in solvent production were obtained compared to those without pH control. These results demonstrate a possible approach combining optimized pH control and D-glucose and D-xylose ratio to increase the fermentation efficiency of lignocellulosic hydrolysate.

Key words: Clostridium acetobutylicum ATCC 824, Xylose, Mixed sugar, pH control

摘要： D-Glucose, L-arabinose, D-mannose, D-xylose, and cellobiose are saccharification products of lignocellulose and important carbon sources for industrial fermentation. The fermentation efficiency with each of the five sugars and the mixture of the two most dominant sugars, D-glucose and D-xylose, was evaluated for acetone- butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum ATCC 824. The utilization efficacy of the five reducing sugars was in the order of D-glucose, L-arabinose, D-mannose, D-xylose and cellobiose. D-Xylose, the second most abundant component in lignocellulosic hydrolysate, was used in the fermentation either as sole carbon source or mixed with glucose. The results indicated that maintaining pH at 4.8, the optimal pH value for solventogenesis, could increase D-xylose consumption when it was the sole carbon source. Different media containing D-glucose and D-xylose at different ratios (1:2, 1:5, 1.5:1, 2:1) were then attempted for the ABE fermentation. When pH was at 4.8 and xylose concentration was five times that of glucose, a 256.9% increase in xylose utilization and 263.7% increase in solvent production were obtained compared to those without pH control. These results demonstrate a possible approach combining optimized pH control and D-glucose and D-xylose ratio to increase the fermentation efficiency of lignocellulosic hydrolysate.

关键词: Clostridium acetobutylicum ATCC 824, Xylose, Mixed sugar, pH control

Wei Jiang, Zhiqiang Wen, Mianbin Wu, Hong Li, Jun Yang, Jianping Lin, Yijun Lin, Lirong Yang, Peilin Cen. The Effect of pH Control on Acetone–Butanol–Ethanol Fermentation by Clostridium acetobutylicum ATCC 824 with Xylose and D-Glucose and D-Xylose Mixture[J]. Chin.J.Chem.Eng., 2014, 22(8): 937-942.

References

[1] T.C. Ezeji, H.P. Blaschek, Biofuel from butanol: advances in genetic and physiologicalmanipulation of clostridia, BioWorld Eur. 2 (2007) 12-15.

[2] Nexant Offices, Biobutanol: the next big biofuel, Chemical Systems with ChemicalStrategies, Nexant, 2009.

[3] C. Weber, A. Farwick, F. Benisch, D. Brat, H. Dietz, T. Subtil, E. Boles, Trends andchallenges in the microbial production of lignocellulosic bioalcohol fuels, Appl.Microbiol. Biotechnol. 87 (4) (2010) 1303-1315.

[4] D.T. Jones, D.R. Woods, Acetone-butanol fermentation revisited, Microbiol. Rev. 50(4) (1986) 484-534.

[5] T.C. Ezeji, N. Qureshi, H.P. Blaschek, Production of acetone butanol (AB) from liquefiedcorn starch, a commercial substrate, using Clostridium beijerinckii coupled withproduct recovery by gas stripping, J. Ind. Microbiol. Biotechnol. 34 (12) (2007)771-777.

[6] K. Shetty, G. Paliyath, A. Pometto, R.E. Levin, Food Biotechnology, Second editionTaylor & Francis Group, Boca Raton, Florida, USA, 2005. 525-551.

[7] N. Qureshi, X.L. Li, S. Hughes, B.C. Saha, M.A. Cotta, Butanol production from cornfiber xylan using Clostridium acetobutylicum, Biotechnol. Prog. 22 (3) (2006)673-680.

[8] N. Qureshi, T.C. Ezeji, Butanol, ‘a superior biofuel’ production from agricultural residues(renewable biomass): recent progress in technology, Biofuels, Bioprod. Biorefin.2 (4) (2008) 319-330.

[9] H.S. Lü, M.M. Ren, M.H. Zhang, Y. Chen, Pretreatment of corn stover using supercritical CO₂ with water-ethanol as co-solvent, Chin. J. Chem. Eng. 21 (5) (2013)551-557.

[10] M.J. Zhang, R.X. Su, W. Qi, R.Y. Du, Z.M. He, Enzymatic hydrolysis of cellulose withdifferent crystallinities studied by means of SEC-MALLS, Chin. J. Chem. Eng. 19 (5)(2011) 773-778.

[11] J. Chen, Y.Wang, G. He, H. Zhang, Z. Zhou, Bioconversion of lignocellulose to ethanol,Sci. Silvae Sin. 43 (5) (2007) 99-105.

[12] J. Kim, D.E. Block, D.A. Mills, Simultaneous consumption of pentose and hexosesugars: an optimal microbial phenotype for efficient fermentation of lignocellulosicbiomass, Appl. Microbiol. Biotechnol. 88 (5) (2010) 1077-1085.

[13] A.E. Kanouni, I. Zerdani, S. Zaafa, M. Znassni, M. Lout, M. Boudouma, The improvementof glucose/xylose fermentation by Clostridium acetobutylicum using calciumcarbonate, World J. Microbiol. Biotechnol. 14 (3) (1998) 431-435.

[14] M.H.W. Husemann, E.T. Papoutsakis, Solventogenesis in Clostridium acetobutylicumfermentation related to carboxylic acid and proton concentrations, Biotechnol.Bioeng. 32 (7) (1988) 843-852.

[15] C. Ren, Y. Gu, S.Y. Hu, Y.Wu, P.Wang, Y.L. Yang, C. Yang, S. Yang,W.H. Jiang, Identificationand inactivation of pleiotropic regulator CcpA to eliminate glucose repressionof xylose utilization in Clostridium acetobutylicum, Metab. Eng. 12 (5) (2010)446-454.

[16] C. Grimmler, C. Held, W. Liebl, A. Ehrenreich, Transcriptional analysis of cataboliterepression in Clostridium acetobutylicum growing on mixtures of d-glucoseand d-xylose, J. Biotechnol. 150 (3) (2010) 315-323.

[17] M.D. Servinsky, J.T. Kiel, N.F. Dupuy, C.J. Sund, Transcriptional analysis of differentialcarbohydrate utilization by Clostridium acetobutylicum, Microbiology 156 (11)(2010) 3478-3491.

[18] T. Millat, H. Janssen, G.J. Thorn, J.R. King, H. Bahl, R.J. Fischer, O.Wolkenhauer, A shiftin the dominant phenotype governs the pH-induced metabolic switch of Clostridiumacetobutylicum in phosphate-limited continuous cultures, Appl. Microbiol. Biotechnol.97 (14) (2013) 6451-6466.

[19] M. Gottwald, G. Gottschalk, The internal pH of Clostridium acetobutylicum and its effecton the shift from acid to solvent formation, Arch. Microbiol. 143 (1) (1985)42-46.

[20] D.B. Kell, M.W. Peck, G. Rodger, J.G. Morris, On the permeability to weak acids andbases of the cytoplasmic membrane of Clostridium pasteurianum, Biochem. Biophys.Res. Commun. 99 (1) (1981) 81-88.

[21] K. Ounine, H. Petitdemange, G. Raval, R. Gay, Regulation and butanol inhibition of DD-xylose and D D-glucose uptake in Clostridium acetobutylicum, Appl. Environ.Microbiol. 49 (4) (1985) 874-878.

[22] I.S. Maddox, E. Steiner, S. Hirsch, S. Wessner, N.A. Gutierrez, J.R. Gapes, K.C. Schuster,The cause of “acid crash” and “acidogenic fermentations” during the batch acetone-butanol-ethanol (ABE-) fermentation process, J. Mol. Microbiol. Biotechnol. 2 (1)(2000) 95-100.

[23] J.-P. Pitkänen, A. Aristidou, L. Salusjärvi, L. Ruohonen, M. Penttilä, Metabolic fluxanalysis of xylose metabolism in recombinant Saccharomyces cerevisiae using continuousculture, Metab. Eng. 5 (1) (2003) 16-31.

[24] E. Boles, S. Müller, F.K. Zimmermann, A multi-layered sensory systemcontrols yeastglycolytic gene expression, Mol. Microbiol. 19 (3) (1996) 641-642.

[25] N.Q. Meinander, I. Boels, B. Hahn-Hägerdal, Fermentation of xylose/glucose mixturesby metabolically engineered Saccharomyces cerevisiae strains expressingXYL1 and XYL2 from Pichia stipitis with and without overexpression of TAL1,Bioresour. Technol. 68 (1) (1999) 79-87.

[26] M. Bertilsson, J. Andersson, G. Lidén, Modeling simultaneous glucose and xylose uptakein Saccharomyces cerevisiae from kinetics and gene expression of sugar transporters,Bioprocess Biosyst. Eng. 31 (4) (2008) 369-377.

[27] M. Bertilsson, K. Olofsson, G. Lidén, Prefermentation improves xylose utilization insimultaneous saccharification and co-fermentation of pretreated spruce, Biotechnol.Biofuels 2 (2009) 8.

[28] M.-R. Ricardo, V.G. Krist, S.M. Anne, S. Gürkan, A mathematical model for simultaneoussaccharification and co-fermentation (SSCF) of C6 and C5 sugars, Chin. J.Chem. Eng. 19 (2) (2011) 185-191.

[29] P.A.M. Claassen, J.B. van Lier, A.M. Lopez Contreras, E.W.J. van Niel, L. Sijtsma, A.J.M.Stams, S.S. de Vries, R.A. Weusthuis, Utilisation of biomass for the supply of energycarriers, Appl. Microbiol. Biotechnol. 52 (6) (1999) 741-755.

The Effect of pH Control on Acetone–Butanol–Ethanol Fermentation by Clostridium acetobutylicum ATCC 824 with Xylose and D-Glucose and D-Xylose Mixture

The Effect of pH Control on Acetone–Butanol–Ethanol Fermentation by Clostridium acetobutylicum ATCC 824 with Xylose and D-Glucose and D-Xylose Mixture

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