SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2022, Vol. 47 ›› Issue (7): 18-30.DOI: 10.1016/j.cjche.2021.05.026

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Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli

Xiaobo Ruan1,2, Sheng Zhang3, Wei Song1,2, Jia Liu2, Xiulai Chen2, Liming Liu2, Jing Wu1   

  1. 1. School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China;
    2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
    3. Tianrui Chemical Co., Ltd, Department of Chemistry, Quzhou 324400, China
  • Received:2021-02-06 Revised:2021-05-02 Online:2022-08-19 Published:2022-07-28
  • Contact: Jing Wu,E-mail:wujing@jiangnan.edu.cn
  • Supported by:
    This work was financially supported by the Fundamental Research Funds for the Central Universities (JUSRP21915), National Natural Science Foundation of China (22008089, 21878126), Provincial Natural Science Foundation of Jiangsu Province (BK20200622), the key technologies Research & Development Program of Jiangsu Province (BE2018623), and the National First-Class Discipline Program of Light Industry Technology and Engineering (LITE2018-20).

Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli

Xiaobo Ruan1,2, Sheng Zhang3, Wei Song1,2, Jia Liu2, Xiulai Chen2, Liming Liu2, Jing Wu1   

  1. 1. School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China;
    2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
    3. Tianrui Chemical Co., Ltd, Department of Chemistry, Quzhou 324400, China
  • 通讯作者: Jing Wu,E-mail:wujing@jiangnan.edu.cn
  • 基金资助:
    This work was financially supported by the Fundamental Research Funds for the Central Universities (JUSRP21915), National Natural Science Foundation of China (22008089, 21878126), Provincial Natural Science Foundation of Jiangsu Province (BK20200622), the key technologies Research & Development Program of Jiangsu Province (BE2018623), and the National First-Class Discipline Program of Light Industry Technology and Engineering (LITE2018-20).

Abstract: For the efficient conversion of L-tyrosine (L-Tyr) to tyrosol, which is an aromatic compound widely used in the pharmaceutical and chemical industries, a novel four-enzyme cascade pathway based on the Ehrlich pathway of Saccharomyces cerevisiae was designed and reconstructed in Escherichia coli. Then, the expression levels of the relevant enzymes were coordinated using a modular approach and gene duplication after the identification of the pyruvate decarboxylase from Candida tropicalis (CtPDC) as the rate-limiting enzymatic step. In situ product removal (ISPR) strategy with XAD4 resins was explored to avoid product inhibition and further improve tyrosol yield. As a result, the titer and conversion rate of tyrosol obtained were 35.7 g·L-1 and 93.6%, respectively, in a 3-L bioreactor. Results presented here provide a potential enzymatic process for industrial production of tyrosol from cheap amino acids.

Key words: Tyrosol, L-tyrosine, Ehrlich pathway, Enzyme cascade, In situ product removal

摘要: For the efficient conversion of L-tyrosine (L-Tyr) to tyrosol, which is an aromatic compound widely used in the pharmaceutical and chemical industries, a novel four-enzyme cascade pathway based on the Ehrlich pathway of Saccharomyces cerevisiae was designed and reconstructed in Escherichia coli. Then, the expression levels of the relevant enzymes were coordinated using a modular approach and gene duplication after the identification of the pyruvate decarboxylase from Candida tropicalis (CtPDC) as the rate-limiting enzymatic step. In situ product removal (ISPR) strategy with XAD4 resins was explored to avoid product inhibition and further improve tyrosol yield. As a result, the titer and conversion rate of tyrosol obtained were 35.7 g·L-1 and 93.6%, respectively, in a 3-L bioreactor. Results presented here provide a potential enzymatic process for industrial production of tyrosol from cheap amino acids.

关键词: Tyrosol, L-tyrosine, Ehrlich pathway, Enzyme cascade, In situ product removal