Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy

doi:10.1016/j.cjche.2022.01.022

Chinese Journal of Chemical Engineering ›› 2023, Vol. 53 ›› Issue (1): 324-331.DOI: 10.1016/j.cjche.2022.01.022

Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy

Xinhai Zhou¹, Dawei Zhou¹, Xinhui Bao¹, Yang Zhang², Jie Zhou¹, Fengxue Xin^1,3, Wenming Zhang^1,3, Xiujuan Qian¹, Weiliang Dong^1,3, Min Jiang^1,3, Katrin Ochsenreither⁴

1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China;
2. Institute of Microbiology, Chinese Academy of Science, Beijing 100101, China;
3. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, China;
4. Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Fritz-Haber-Weg4, Karlsruhe 76131, Germany

Received:2021-08-20 Revised:2022-01-18 Online:2023-04-08 Published:2023-01-28
Contact: Xiujuan Qian,E-mail:xiujuanqian@njtech.edu.cn;Weiliang Dong,E-mail:dwl@njtech.edu.cn
Supported by:
This work was supported by the National Key Research & Development Program of China (2021YFC2101500, 2018YFA0902200), National Natural Science Foundation of China (22008115, 21978130), Jiangsu Province Natural Science Foundation for Youths (SBK2020044721), Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund Project (CX(21)3120), Jiangsu Planned Projects for Postdoctoral Research Funds (2021K085A), China Postdoctoral Science Foundation (2020M671467), and Postdoctoral Research Funding Program of Jiangsu Province (2021K085A).

Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy

Xinhai Zhou¹, Dawei Zhou¹, Xinhui Bao¹, Yang Zhang², Jie Zhou¹, Fengxue Xin^1,3, Wenming Zhang^1,3, Xiujuan Qian¹, Weiliang Dong^1,3, Min Jiang^1,3, Katrin Ochsenreither⁴

1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China;
2. Institute of Microbiology, Chinese Academy of Science, Beijing 100101, China;
3. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, China;
4. Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Fritz-Haber-Weg4, Karlsruhe 76131, Germany

通讯作者: Xiujuan Qian,E-mail:xiujuanqian@njtech.edu.cn;Weiliang Dong,E-mail:dwl@njtech.edu.cn
基金资助:
This work was supported by the National Key Research & Development Program of China (2021YFC2101500, 2018YFA0902200), National Natural Science Foundation of China (22008115, 21978130), Jiangsu Province Natural Science Foundation for Youths (SBK2020044721), Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund Project (CX(21)3120), Jiangsu Planned Projects for Postdoctoral Research Funds (2021K085A), China Postdoctoral Science Foundation (2020M671467), and Postdoctoral Research Funding Program of Jiangsu Province (2021K085A).

Abstract

Abstract: Palmitoleic acid (POA) can be naturally found only in few oil seeds and has significant applications in pharmaceutical industry. Recently, the isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 was identified with high content of POA in its intracellular lipid (13.80%). In this study, process optimization focused on dissolved oxygen regulation to improve POA production was conducted. Dynamic agitation was found to do significant enhancement on POA-rich lipid production than aeration regulation. Under the best condition of 1000 r·min^-1 of agitation and 1 vvm (airvolume/culture volume/min) of aeration, no ethanol was detected during the whole fermentation process, while a dry biomass concentration of 44.80 g·L^-1 with 13.43 g·L^-1 of lipid and 2.93 g·L^-1 of POA was achieved. Transcription analysis revealed that the ethanol synthetic pathway was downregulated under the condition of high agitation, while the expression of the key enzymes responsible for lipid and POA accumulation were enhanced.

Key words: Palmitoleic acid production, Scheffersomyces segobiensis, Dissolved oxygen, Ethanol, Bioprocess, Bioreactors

摘要： Palmitoleic acid (POA) can be naturally found only in few oil seeds and has significant applications in pharmaceutical industry. Recently, the isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 was identified with high content of POA in its intracellular lipid (13.80%). In this study, process optimization focused on dissolved oxygen regulation to improve POA production was conducted. Dynamic agitation was found to do significant enhancement on POA-rich lipid production than aeration regulation. Under the best condition of 1000 r·min^-1 of agitation and 1 vvm (airvolume/culture volume/min) of aeration, no ethanol was detected during the whole fermentation process, while a dry biomass concentration of 44.80 g·L^-1 with 13.43 g·L^-1 of lipid and 2.93 g·L^-1 of POA was achieved. Transcription analysis revealed that the ethanol synthetic pathway was downregulated under the condition of high agitation, while the expression of the key enzymes responsible for lipid and POA accumulation were enhanced.

关键词: Palmitoleic acid production, Scheffersomyces segobiensis, Dissolved oxygen, Ethanol, Bioprocess, Bioreactors

Xinhai Zhou, Dawei Zhou, Xinhui Bao, Yang Zhang, Jie Zhou, Fengxue Xin, Wenming Zhang, Xiujuan Qian, Weiliang Dong, Min Jiang, Katrin Ochsenreither. Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy[J]. Chinese Journal of Chemical Engineering, 2023, 53(1): 324-331.

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Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy

Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy

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