Biogas by two-stage microbial anaerobic and semi-continuous digestion of Chinese cabbage waste

doi:10.1016/j.cjche.2015.03.001

Abstract

Abstract: Anaerobic digestion of Chinese cabbage waste was investigated through a pilot-scale two-stage digester at a mesophilic temperature of 37 ℃. In the acidification digester, the main product was acetic acid, with the maximum concentration of 4289 mg·L^-1 on the fourth day, accounting for 50.32% of total volatile fatty acids. The oxidation reduction potential (ORP) and NH₄⁺-N level decreased gradually with hydraulic retention time (HRT) of acidification. In the second digestion phase, the maximum methanogenic bacterial concentration reached 9.6×10¹⁰ ml^-1 at the organic loading rate (OLR) of 3.5-4 kg VS·m^-3, with corresponding HRT of 12-16 days. Accordingly, the optimal biogas production was 0.62 m³·(kg VS)^-1, with methane content of 65%-68%. ORP and NH₄⁺-N levels in the methanizer remained between -500 and -560 mV and 2000-4500 mg·L^-1, respectively. Methanococcus and Methanosarcina served as the main methanogens in the anaerobic digester.

Key words: Anaerobic digestion, Semi-continuous, Two-stage, Biogas, Chinese cabbage waste, Microbial ecology

摘要： Anaerobic digestion of Chinese cabbage waste was investigated through a pilot-scale two-stage digester at a mesophilic temperature of 37 ℃. In the acidification digester, the main product was acetic acid, with the maximum concentration of 4289 mg·L^-1 on the fourth day, accounting for 50.32% of total volatile fatty acids. The oxidation reduction potential (ORP) and NH₄⁺-N level decreased gradually with hydraulic retention time (HRT) of acidification. In the second digestion phase, the maximum methanogenic bacterial concentration reached 9.6×10¹⁰ ml^-1 at the organic loading rate (OLR) of 3.5-4 kg VS·m^-3, with corresponding HRT of 12-16 days. Accordingly, the optimal biogas production was 0.62 m³·(kg VS)^-1, with methane content of 65%-68%. ORP and NH₄⁺-N levels in the methanizer remained between -500 and -560 mV and 2000-4500 mg·L^-1, respectively. Methanococcus and Methanosarcina served as the main methanogens in the anaerobic digester.

关键词: Anaerobic digestion, Semi-continuous, Two-stage, Biogas, Chinese cabbage waste, Microbial ecology

Xiaoying Dong, Lijie Shao, Yan Wang, Wei Kou, Yanxin Cao, Dalei Zhang. Biogas by two-stage microbial anaerobic and semi-continuous digestion of Chinese cabbage waste[J]. Chin.J.Chem.Eng., 2015, 23(5): 847-852.

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