Amino Acids Production from Fish Proteins Hydrolysis in Subcritical Water

›› 2008, Vol. 16 ›› Issue (3): 456-460.

Amino Acids Production from Fish Proteins Hydrolysis in Subcritical Water

朱宪, 朱超, 赵亮, 程洪斌

Department of Chemical Engineering, School of Environment and Chemical Engineering, Shanghai University, Shanghai 201800, China

收稿日期:2007-09-25 修回日期:2008-03-01 出版日期:2008-06-28 发布日期:2008-06-28
通讯作者: ZHU Xian,E-mail:xzhu@staff.shu.edu.cn
基金资助:
National Natural Science Foundation of China (50578091);Shanghai Leading Academic Discipline Project(T-105)

Amino Acids Production from Fish Proteins Hydrolysis in Subcritical Water

ZHU Xian, ZHU Chao, ZHAO Liang, CHENG Hongbin

Department of Chemical Engineering, School of Environment and Chemical Engineering, Shanghai University, Shanghai 201800, China

Received:2007-09-25 Revised:2008-03-01 Online:2008-06-28 Published:2008-06-28
Supported by:
National Natural Science Foundation of China (50578091);Shanghai Leading Academic Discipline Project(T-105)

摘要/Abstract

摘要： The hydrolysis technology and reaction kinetics for amino acids production from fish proteins in subcritical water reactor without catalysts were investigated in a reactor with volume of 400 ml under the conditions of reaction temperature from 180-320℃,pressure from 5-26 MPa,and time from 5-60 min.The quality and quantity of amino acids in hydrolysate were determined by bioLiquid chromatography,and 17 kinds of amino acids were obtained.For the important 8 amino acids,the experiments were conducted to examine the effects of reaction temperature,pressure and time on amino acids yield.The optimum conditions for high yield are obtained from the experimental results.It is found that the nitrogen and carbon dioxide atmosphere should be used for leucine,isoleucine and histidine production while the air atmosphere might be used for other amino acids.The reaction time of 30 min and the experimental temperature of 220℃,240℃ and 260℃ were adopted for reaction kinetic research.The total yield of amino acids versus reaction time have been examined experimentally.According to these experimental data and under the condition of water excess,the macroscopic reaction kinetic equation of fish proteins hydrolysis was obtained with the hydrolysis reaction order of 1.615 and the rate constants being 0.0017,0.0045 and 0.0097 at 220℃,240℃ and 260℃ respectively.The activation energy is 145.1 kJ·mol^-1.

关键词: biomass, subcritical water, hydrolysis, reaction kinetics, amino acids

Abstract: The hydrolysis technology and reaction kinetics for amino acids production from fish proteins in subcritical water reactor without catalysts were investigated in a reactor with volume of 400 ml under the conditions of reaction temperature from 180-320℃,pressure from 5-26 MPa,and time from 5-60 min.The quality and quantity of amino acids in hydrolysate were determined by bioLiquid chromatography,and 17 kinds of amino acids were obtained.For the important 8 amino acids,the experiments were conducted to examine the effects of reaction temperature,pressure and time on amino acids yield.The optimum conditions for high yield are obtained from the experimental results.It is found that the nitrogen and carbon dioxide atmosphere should be used for leucine,isoleucine and histidine production while the air atmosphere might be used for other amino acids.The reaction time of 30 min and the experimental temperature of 220℃,240℃ and 260℃ were adopted for reaction kinetic research.The total yield of amino acids versus reaction time have been examined experimentally.According to these experimental data and under the condition of water excess,the macroscopic reaction kinetic equation of fish proteins hydrolysis was obtained with the hydrolysis reaction order of 1.615 and the rate constants being 0.0017,0.0045 and 0.0097 at 220℃,240℃ and 260℃ respectively.The activation energy is 145.1 kJ·mol^-1.

Key words: biomass, subcritical water, hydrolysis, reaction kinetics, amino acids

朱宪, 朱超, 赵亮, 程洪斌. Amino Acids Production from Fish Proteins Hydrolysis in Subcritical Water[J]. , 2008, 16(3): 456-460.

ZHU Xian, ZHU Chao, ZHAO Liang, CHENG Hongbin. Amino Acids Production from Fish Proteins Hydrolysis in Subcritical Water[J]. , 2008, 16(3): 456-460.

参考文献

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Amino Acids Production from Fish Proteins Hydrolysis in Subcritical Water

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