HPLC Analysis of Egg Yolk Phosphatidylcholine by Evaporative Light Scattering Detector

Chinese Journal of Chemical Engineering ›› 2012, Vol. 20 ›› Issue (4): 665-672.

HPLC Analysis of Egg Yolk Phosphatidylcholine by Evaporative Light Scattering Detector

周婧, 胡仙妹, 王田心, 梁浩, 袁其朋

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China

收稿日期:2011-03-30 修回日期:2011-09-24 出版日期:2012-08-28 发布日期:2012-09-15
通讯作者: YUAN Qipeng, E-mail:yuanqp@mail.buct.edu.cn

HPLC Analysis of Egg Yolk Phosphatidylcholine by Evaporative Light Scattering Detector

ZHOU Jing, HU Xianmei, WANG Tianxin, LIANG Hao, YUAN Qipeng

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China

Received:2011-03-30 Revised:2011-09-24 Online:2012-08-28 Published:2012-09-15

摘要/Abstract

摘要： Egg yolk phosphatidylcholine(EYPC) is being widely used in food and pharmaceutical industries nowadays owing to its surface activity,pharmaceutical usefulness,and so on.Common determination methods of phospholipids were based on the American Oil Chemists' Society(AOCS) Official Method Ja7b-91,in which n-hexane/2-propanol/acetate buffer was used as the mobile phase.In order to achieve desired results,gradient elu-tion or buffer solution was used,which made the detection process more complicated.Moreover,water or buffer solution could affect the silica gel column both on its lifespan and the separation efficiency significantly.In this study,different mobile phase and detector were used to simplify EYPC analyzing process instead of using water within the mobile phase.The optimized HPLC operating conditions are as follows:pure methanol as a mobile phase,flow rate of 1.0 ml·min^-1,silica gel column(250 mm×4.6 mm,5 μm,Inertsil GL^TM),column temperature 30℃ and low temperature evaporative light scattering detector(40℃,0.35 MPa) as used.Under this optimal condition,the linear relative coefficient of the standard curve is 0.998 and the recovery was in the range of 96.83%-101.58% with a relative standard deviation of 1.79%(n=6).

关键词: egg yolk phosphatidylcholine, high performance liquid chromatography, evaporative light scattering detector

Abstract: Egg yolk phosphatidylcholine(EYPC) is being widely used in food and pharmaceutical industries nowadays owing to its surface activity,pharmaceutical usefulness,and so on.Common determination methods of phospholipids were based on the American Oil Chemists' Society(AOCS) Official Method Ja7b-91,in which n-hexane/2-propanol/acetate buffer was used as the mobile phase.In order to achieve desired results,gradient elu-tion or buffer solution was used,which made the detection process more complicated.Moreover,water or buffer solution could affect the silica gel column both on its lifespan and the separation efficiency significantly.In this study,different mobile phase and detector were used to simplify EYPC analyzing process instead of using water within the mobile phase.The optimized HPLC operating conditions are as follows:pure methanol as a mobile phase,flow rate of 1.0 ml·min^-1,silica gel column(250 mm×4.6 mm,5 μm,Inertsil GL^TM),column temperature 30℃ and low temperature evaporative light scattering detector(40℃,0.35 MPa) as used.Under this optimal condition,the linear relative coefficient of the standard curve is 0.998 and the recovery was in the range of 96.83%-101.58% with a relative standard deviation of 1.79%(n=6).

Key words: egg yolk phosphatidylcholine, high performance liquid chromatography, evaporative light scattering detector

周婧, 胡仙妹, 王田心, 梁浩, 袁其朋. HPLC Analysis of Egg Yolk Phosphatidylcholine by Evaporative Light Scattering Detector[J]. Chinese Journal of Chemical Engineering, 2012, 20(4): 665-672.

ZHOU Jing, HU Xianmei, WANG Tianxin, LIANG Hao, YUAN Qipeng. HPLC Analysis of Egg Yolk Phosphatidylcholine by Evaporative Light Scattering Detector[J]. Chin.J.Chem.Eng., 2012, 20(4): 665-672.

参考文献

1 Shen,X.J.,Lai,B.S.,"Development and utilization of yolk phospholipid",Chinese Journal of Pharmaceuticals,25(5),231-233 (1994).
2 Chi,Y.J.,Lin,S.Y.,"Research advance in the extraction and application of egg yolk lecithin",Food and Fermentation Industries,28(5),50-53(2002).
3 Hradec,J.,Dufek,P.,"Isolation and quantitation of phosphatidylcholine by reversed-phase liquid-liquid extraction",J.Chromatogr. B,703(1-2),259-262(1997).
4 Cheng,W.L.,Holmes-McNary,M.Q.,Mar,M.H.,Lien,E.L.,Zeisel, S.H.,"Bioavailability of choline and choline esters from milk in rat pups",The Journal of Nutritional Biochemistry,7(8),457-464 (1996).
5 Jiang,Y.Z.,Noh,S.K.,Koo,S.I.,"Egg phosphatidylcholine decreases the lymphatic absorption of cholesterol in rats",Journal of Nutrition,131(9),2358-2363(2001).
6 Palacios,L.E.,Wang,T.,"Egg yolk lipid fractionation and lecithin characterization",Journal of the American Oil Chemists'Society,82 (8),571-578(2005).
7 Carelli,A.A.,Brevedan,M.I.V.,Crapiste,G.H.,"Quantitative determination of phospholipids in sunflower oil",Journal of the American Oil Chemists'Society,74(5),511-514(1997).
8 Balazs,P.E.,Schimit,P.L.,Szuhaj,B.F.,"High performance liquid chromatographic separations of soy phospholipids",Journal of the American Oil Chemists'Society,73(2),193-197(1996).
9 Hax,W.M.,van Kessel,W.S.,"High-performance liquid chromatographic separation and photometric detection of phospholipids",J. Chromatogr.A,142(11),735-741(1977).
10 Szücs,R.,Verleysen,K.,Duchateau,G.S.,Sandra,P.,Vandeginste, B.G.M.,"Analysis of phospholipids in lecithins comparison between micellar electrokinetic chromatography and high-performance liquid chromatography",J.Chromatogr.A,738(1),25-29(1996).
11 Sala Vila,A.,Castellote-Bargalló,A.I.,Rodríguez-Palmero-Seuma, M.,López-Sabater,M.C.,"High-performance liquid chromatography with evaporative light-scattering detection for the determination of phospholipid classes in human milk,infant formulas and phospholipid sources of long-chain polyunsaturated fatty acids",J. Chromatogr.A,1008(1),73-80(2003).
12 Rombaut,R.,Dewettinck,K.,Camp,J.V.,"Phospho-and sphingolipid content of selected dairy products as determined by HPLC coupled to an evaporative light scattering detector",Journal of Food Composition and Analysis,20(3-4),308-312(2007).
13 Yoon,T.H.,Kim,I.H.,"Phosphatidylcholine isolation from egg yolk phospholipids by high-performance liquid chromatography",J. Chromatogr.A,949(1-2),209-216(2002).
14 Teberikler,L.,Koseoglu,S.,Akgerman,A.,"Selective extraction of phosphatidylcholine from lecithin by supercritical carbon dioxide/ethanol mixture",Journal of the American Oil Chemists'Society,78(2),115-119(2001).
15 Huang,J.Z.,Lin,S.Y.,Gong,X.T.,Li,D.,Liu,J.B.,"Study on optimization of extraction technology of egg yolk lecithin by uniform design method",Chinese Journal of Food Science,29(10),257-259 (2008).
16 Patil Vilas,V.,Galge Revanappa,V.,Thorat Bhaskar,N.,"Extraction and purification of phosphatidylcholine from soyabean lecithin",Sep. Purif.Technol.,75(2),138-144(2010).
17 Rombaut,R.,Camp,J.V.,Dewettinck,K.,"Analysis of phosphoand sphingolipids in dairy products by a new HPLC method",Journal of Dairy Science,88(2),482-488(2005).
18 Li,G.C.,Wang,Y.H.,Wu,R.A.,WANG,S.C.,"Analysis of phospholipids in egg yolk by offline HPLC-MALDI-TOF-MS",Chinese Journal of Analysis Laboratory,28(4),30-33(2009).
19 Zhang,Y.Y.,Yang,Y.W.,Wu,C.J.,Ren,Q.L.,"Progress of phospholipids determination",Chinese Journal of Pharmaceuticals,37 (1),58-60(2006).
20 Helmerich,G.,Koehler,P.,"Comparison of methods for the quantitative determination of phospholipids in lecithin and flour improvers",Journal of Agricultural Food Chemistry,51(23),6645-6651 (2003).
21 Wang,Y.,Wang,X.G.,Hu,X.Y.,"Methods for determination and analysis of phospholipids composition and content",China Oils and Fats,27(4),64-67(2002).
22 Zhang,W.N.,He,H.B.,Feng,Y.Q.,"Separation of phosphatidylcholine and phosphatidylethanolamine by using high performance displacement chromatography",J.Chromatogr.A,1036(2),145-154 (2004).
23 Grizard,G.,Sion,B.,Bauchart,D.,"Separation and quantification of cholesterol and major phospholipid classes in human semen by high-performance liquid chromatography and light-scattering detection",J.Chromatogr.B,740(6),101-107(2000).
24 Zhu,J.L.,Tang,Y.F.,Li,J.H.,"Analysis of sucrose esters with long acyl chain by coupling of HPLC-ELSD with ESI-MS system",Chin. J.Chem.Eng.,17(6),1032-1037(2009).

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HPLC Analysis of Egg Yolk Phosphatidylcholine by Evaporative Light Scattering Detector

HPLC Analysis of Egg Yolk Phosphatidylcholine by Evaporative Light Scattering Detector

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