Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

›› 2009, Vol. 17 ›› Issue (2): 278-285.

Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

许勤勤¹, 吕龙贤¹, 陈少云¹, 郑静¹, 郑高利², 李永泉¹

1. College of Life Sciences, Zhejiang University, Hangzhou 310058, China;
2. Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China

收稿日期:2008-06-23 修回日期:2008-11-10 出版日期:2009-04-28 发布日期:2009-04-28
通讯作者: LI Yongquan,E-mail:lyq@zju.edu.cn
基金资助:
Supported by the Research Project of Science and Technology of Zhejiang Province,China (2005C23027);the National High Technology Research and Development Program of China (2007AA021506);the Natural Science Foundation of Zhejiang Province (R207609)

Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

XU Qinqin¹, LÜ Longxian¹, CHEN Shaoyun¹, ZHENG Jing¹, ZHENG Gaoli², LI Yongquan¹

1. College of Life Sciences, Zhejiang University, Hangzhou 310058, China;
2. Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China

Received:2008-06-23 Revised:2008-11-10 Online:2009-04-28 Published:2009-04-28
Supported by:
Supported by the Research Project of Science and Technology of Zhejiang Province,China (2005C23027);the National High Technology Research and Development Program of China (2007AA021506);the Natural Science Foundation of Zhejiang Province (R207609)

摘要/Abstract

摘要： A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence.The optimal medium composition(g·L^-1),containing sucrose 66.0,yeast powder 10.0,silkworm chrysalises digest 30.0,MgSO₄·7H₂O 0.4,and KH₂PO₄ 0.4,was found using fractional factorial design and a central composite design,and the optimization of cultural conditions obtained a result of seed age 6 days,inoculum size 6%(by volume),initial pH 5.6,temperature 24℃,shaking speed 160 r·min^-1 by one-factor-at-a-time method.The maximum biomass reached about 20.2 g·L^-1 after 90 hours culture under the optimal conditions.Elementary pharmacological activities showed that mycelia of C.ophioglossoides L2 from submerged culture promoted uterus growth in estrogendepleted mice.In the 15-litre scale-up fermentation,the mycelial biomass was around 19.1 g·L^-1,indicating a promising prospect for this biotechnology and the potency to develop its medical value.

关键词: Cordyceps ophioglossoides, identification, fermentation, optimization, fractional factorial design, central composite design

Abstract: A strain isolated from the fruiting body of a fungus parasitized on Elaphomyces was identified as Cordyceps ophioglossoides based on the morphological characteristics and the analysis of ITS-5.8s rDNA sequence.The optimal medium composition(g·L^-1),containing sucrose 66.0,yeast powder 10.0,silkworm chrysalises digest 30.0,MgSO₄·7H₂O 0.4,and KH₂PO₄ 0.4,was found using fractional factorial design and a central composite design,and the optimization of cultural conditions obtained a result of seed age 6 days,inoculum size 6%(by volume),initial pH 5.6,temperature 24℃,shaking speed 160 r·min^-1 by one-factor-at-a-time method.The maximum biomass reached about 20.2 g·L^-1 after 90 hours culture under the optimal conditions.Elementary pharmacological activities showed that mycelia of C.ophioglossoides L2 from submerged culture promoted uterus growth in estrogendepleted mice.In the 15-litre scale-up fermentation,the mycelial biomass was around 19.1 g·L^-1,indicating a promising prospect for this biotechnology and the potency to develop its medical value.

Key words: Cordyceps ophioglossoides, identification, fermentation, optimization, fractional factorial design, central composite design

许勤勤, 吕龙贤, 陈少云, 郑静, 郑高利, 李永泉. Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth[J]. , 2009, 17(2): 278-285.

XU Qinqin, LÜ Longxian, CHEN Shaoyun, ZHENG Jing, ZHENG Gaoli, LI Yongquan. Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth[J]. , 2009, 17(2): 278-285.

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Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

Isolation of Cordyceps ophioglossoides L2 from Fruit Body and Optimization of Fermentation Conditions for Its Mycelial Growth

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