Antioxidant and Antimicrobial Activities of Echinacea (Echinacea purpurea L.) Extracts Obtained by Classical and Ultrasound Extraction

›› 2009, Vol. 17 ›› Issue (3): 478-483.

Antioxidant and Antimicrobial Activities of Echinacea (Echinacea purpurea L.) Extracts Obtained by Classical and Ultrasound Extraction

Ivana Stanisavljević¹, Saša Stojičević², Dragan Veličković³, Vlada Veljković¹, Miodrag Lazić¹

1. Faculty of Technology, University of Niš, Bulevar oslobodjenja 124, 16000 Leskovac, Serbia;
2. Chemical Industry Nevena A.D., Djordja Stamenkovića St.,16000 Leskovac, Serbia;
3. Zdravlje-Actavis, Vlajkova 199, 16000 Leskovac, Serbia

收稿日期:2008-06-25 修回日期:2009-03-19 出版日期:2009-06-28 发布日期:2009-06-28
通讯作者: Ivana Stanisavljević,E-mail:icaneca@gmail.com
基金资助:
Supported by the Ministry of Science and Environmental Protection,Republic of Serbia (142073B)

Antioxidant and Antimicrobial Activities of Echinacea (Echinacea purpurea L.) Extracts Obtained by Classical and Ultrasound Extraction

Ivana Stanisavljević¹, Saša Stojičević², Dragan Veličković³, Vlada Veljković¹, Miodrag Lazić¹

1. Faculty of Technology, University of Niš, Bulevar oslobodjenja 124, 16000 Leskovac, Serbia;
2. Chemical Industry Nevena A.D., Djordja Stamenkovića St.,16000 Leskovac, Serbia;
3. Zdravlje-Actavis, Vlajkova 199, 16000 Leskovac, Serbia

Received:2008-06-25 Revised:2009-03-19 Online:2009-06-28 Published:2009-06-28
Supported by:
Supported by the Ministry of Science and Environmental Protection,Republic of Serbia (142073B)

摘要/Abstract

摘要： Antioxidant and antimicrobial activities of Echinacea purpurea L. (Asteraceae) extracts obtained by classical and ultrasound solvent extraction were compared. The dry aerial part of plant was extracted by 70% ethanol at a solid-to-liquid ratio of 1:10 (m/v) and 25℃. The extract obtained by classical solvent extraction contained 29% larger amounts of phenolic compounds and 20% higher content of flavonoids. 2,2-diphenyl-1-picril hydrazyl radical (DPPH) scavenging reached 93.6% and the values of EC50 were (34.16±0.65)μg·ml^-1 and (65.48±1.12) μg·ml^-1 for the extracts obtained by the classical and ultrasound extractions, respectively. The extracts, independent of the extraction technique applied, showed a considerable growth inhibition on Candida albicans and Saccharomyces cerevisiae, while no growth inhibition zones were observed for Aspergillus niger. The diameters of inhibition zone observed for all the microorganisms were larger for extracts obtained by classical extraction than those by ultrasound extraction.

关键词: antioxidant activity, antimicrobial activity, Echinacea purpurea L., total phenols, flavonoids, extraction

Abstract: Antioxidant and antimicrobial activities of Echinacea purpurea L. (Asteraceae) extracts obtained by classical and ultrasound solvent extraction were compared. The dry aerial part of plant was extracted by 70% ethanol at a solid-to-liquid ratio of 1:10 (m/v) and 25℃. The extract obtained by classical solvent extraction contained 29% larger amounts of phenolic compounds and 20% higher content of flavonoids. 2,2-diphenyl-1-picril hydrazyl radical (DPPH) scavenging reached 93.6% and the values of EC50 were (34.16±0.65)μg·ml^-1 and (65.48±1.12) μg·ml^-1 for the extracts obtained by the classical and ultrasound extractions, respectively. The extracts, independent of the extraction technique applied, showed a considerable growth inhibition on Candida albicans and Saccharomyces cerevisiae, while no growth inhibition zones were observed for Aspergillus niger. The diameters of inhibition zone observed for all the microorganisms were larger for extracts obtained by classical extraction than those by ultrasound extraction.

Key words: antioxidant activity, antimicrobial activity, Echinacea purpurea L., total phenols, flavonoids, extraction

Ivana Stanisavljević, Saša Stojičević, Dragan Veličković, Vlada Veljković, Miodrag Lazić. Antioxidant and Antimicrobial Activities of Echinacea (Echinacea purpurea L.) Extracts Obtained by Classical and Ultrasound Extraction[J]. , 2009, 17(3): 478-483.

参考文献

1 Speroni, E., Govonib, P., Guizzardib, S., Renzullia, C., Guerra, M.C., “Anti-inflammatory and cicatrizing activity of Echinacea pallida Nutt. root extract”, J. Ethnopharmacol., 79, 265-272(2002).
2 Wills, R.B.H., Stuart, D.L., “Alkylamide and cichoric acid levels in Echinacea purpurea grown in Australia”, Food Chem., 67, 385-388(1999).
3 NCCAM Publication No. D271 July 2005, http://nccam.nih.gov/ health/echinacea/,(17.05.2007).
4 Barrett, B., “Medicinal properties of Echinacea:A critical review”, Phytomedicine, 10, 66-86(2003).
5 Luo, X.B., Chen, B., Yao, S.Z., Zeng, J.G., “Simultaneous analysis of caffeic acid derivatives and alkamides in roots and extracts of Echinacea purpurea by high-performance liquid chromatography-photodiode array detection-electrospray mass spectrometry”, J. Chromatogr. A, 986, 73-81(2003).
6 Percival, S.S., “Use of Echinacea in medicine”, Biochem. Pharmacology, 60, 155-158(2000).
7 Pellati, F., Benvenuti, S., Magro, L., Melegari, M., Soragni, F., “Analysis of phenolic compounds and radical scavenging activity of Echinacea spp.”, J. Pharm. Biomed. Anal., 35, 289-301(2004).
8 Cech, N.B., Eleazer, M.S., Shoffner, L.T., Crosswhite, M.R., Davis, A.C., Mortenson, A.M., “High performance liquid chromatography/electrospray ionization mass spectrometry for simultaneous analysis of alkamides and caffeic acid derivatives from Echinacea purpurea extracts”, J. Chromatogr. A, 1103, 219-228(2006).
9 NSF International(2004), INA Methods, Phenolics in Echinacea by HPLC, ttp://www.nsf.org/business/ina/echinacea.asp?program=INA,(26.09.2007).
10 Pugh, N.D., Balachandran, P., Lata H., Dayan, F.E., Joshi, V., Bedir, E., Makino, T., Moraes, R., Khan, I., Pasco, D.S., “Melanin:dietary mucosal immune modulator from Echinacea and other botanical supplements”, Int. Immunopharmacol., 5, 637-647(2005).
11 Bergeron, C., Livesey, J.F., Awang, D.V.C., Arnason, J.T., Rana, J.B., Baum, R., Letchamo, W., “A quantitative HPLC method for the quality assurance of Echinacea products on the North American Market”, Phytochem. Anal., 11, 207-215(2000).
12 Dalby-Brown, L., Barsett, H., Landbo, A.R., Meyer, A.S., Molgaard, P., “Synergistic antioxidative effects of alkamides, caffeic acid derivatives, and polysaccharide fractions from Echinacea purpurea on in vitro oxidation of human low-density lipoproteins”, J. Agric. Food Chem., 53, 9413-9423(2005).
13 Hu, C., Kitts, D.D., “Studies on the antioxidant activity of Echinacea root extract”, J. Agric. Food Chem., 48, 1466-1472(2000).
14 Duff Sloley, B., Urichuk, L.J., Tywin, C., Coutts, R.T., Pang, P.K.T., Shan, J.J., “Comparison of chemical components and antioxidant capacity of different Echinacea species”, J. Pharm. Pharmacol., 53, 849-857(2001).
15 Pietta, P., Simonetti, P., Mauri, P., “Antioxidant activity of selected medicinal plants”, J. Agric. Food Chem., 46, 4487-4490(1998).
16 Cervellati, R., Renzulli, C., Guerra, M.C., Speroni, E., “Evaluation of antioxidant activity of some natural polyphenolic compounds using the Briggs-Rauscher reaction method”, J. Agric. Food Chem., 50, 7504-7509(2002).
17 Burda, S., Oleszek, W., “Antioxidant and antiradical activities of flavonoids”, J. Agric. Food Chem., 49, 2774-2779(2001).
18 Velioglu, Y.S., Mazza, G., Gao, L., Oomah, B.D., “Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products”, J. Agric. Food Chem., 46, 4113-4117(1998).
19 See, D.M., Broumand, N., Sahl, L., Tilles, J.G., “In vitro effects of echinacea and ginseng on natural killer and antibody-dependent cell cytotoxicity in healthy subjects and chronic fatigue syndrome or acquired immunodeficiency syndrome patients”, lmmunopharmacology, 35, 229-235(1997).
20 Binns, S.E., Purgina, B., Bergeron, C., Smith, M.L., Ball, L., Baum, B.R., Arnason, J.T., “Light-mediated antifungal activity of echinacea Extracts”, Planta Med., 66, 241-244(2000).
21 Perry, N.B., Burgess, E.J., Glennie, V.A., “Echinacea standardization:Analytical methods for phenolic compounds and typical levels in medicinal species”, J. Agric. Food Chem., 49, 1702-1706(2001).
22 Wanga, C., Chiaoa, M., Yena, P., Huanga, W., Houa, C., Chiena, S., Yeha, K., Yanga, W., Shyur, L., Yang, N., “Modulatory effects of Echinacea purpurea extracts on human dendritic cells:A celland gene-based study”, Genomics, 88, 801-808(2006).
23 Binns, S.E., Livesey, J.F., Arnason, J.T., Baum, B.R., “Phytochemical variation in echinacea from roots and flowerheads of wild and cultivated populations”, J. Agric. Food Chem., 50, 3673-3687(2002).
24 Kim, H., Durance, T.D., Scaman, C.H., Kitts, D.D., “Retention of alkamides in dried Echinacea purpurea”, J. Agric. Food Chem., 48, 4182-4192(2000).
25 Thygesen, L., Thulin, J., Mortensen, A., Skibsted, L.H., Molgaard, P., “Antioxidant activity of cichoric acid and alkamides from Echinacea purpurea alone and in combination”, Food Chem., 101, 74-81(2007).
26 Choi, C.W., Kim, S.C., Hwang, S.S., Choi, B.K., Ahn, H.J., Lee, M.Y., Park, S.H., Kim, S.K., “Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison”, Plant Sci., 163, 1161-1168(2002).
27 Singleton, V.L., Rossi, J.A., “Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents”, Am. J. Enol. Vitic., 16, 144-158(1965).
28 Chang, C., Yang, M., Wen, H., Chern, J., “Estimation of total flavonoid content in propolis by two complementary colorimetric methods”, J. Food Drug Anal., 10, 178-182(2002).
29 NCCLS(National Committee for Clinical Laboratory Standards), Performance Standards for Antimicrobial Susceptibility Testing 9th International Supplement, Wayne, PA, M100-S9(1999).
30 Pietta, P., “Flavonoids as antioxidants”, J. Nat. Prod., 63, 1035-1042(2000).
31 Paniwnyk, L., Beaufoy, E., Lorimer, J.P., Mason, T.J., “The extraction of rutin from flower buds of Sophora japonica”, Ultrason. Sonochem., 8, 299-301(2001).
32 Pétrier, C., Francony, A., “Ultrasonic waste-water treatment:incidence of ultrasonic frequency on the rate of phenol and carbon tetrachloride degradation”, Ultrason. Sonochem, 4, 295-300(1997).

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Antioxidant and Antimicrobial Activities of Echinacea (Echinacea purpurea L.) Extracts Obtained by Classical and Ultrasound Extraction

Antioxidant and Antimicrobial Activities of Echinacea (Echinacea purpurea L.) Extracts Obtained by Classical and Ultrasound Extraction

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