Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

doi:10.1016/S1004-9541(13)60627-8

Chin.J.Chem.Eng. ›› 2013, Vol. 21 ›› Issue (11): 1291-1295.DOI: 10.1016/S1004-9541(13)60627-8

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

YANG Jun¹, ZHU Li¹, FU Weiqi², LIN Yijun¹, LIN Jianping¹, CEN Peilin¹

1 Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 Center for Systems Biology, University of Iceland, Reykjavik, Iceland

Received:2012-06-04 Revised:2013-01-14 Online:2013-11-26 Published:2013-11-28
Contact: LIN Jianping
Supported by:
Supported by the National Natural Science Foundation of China (20306026 and 20876141) and the National Basic Research Program of China (2007CB707805).

Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

杨俊¹, 朱力¹, 傅维琦², 林逸君¹, 林建平¹, 岑沛霖¹

1 Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 Center for Systems Biology, University of Iceland, Reykjavik, Iceland

通讯作者: LIN Jianping
基金资助:
Supported by the National Natural Science Foundation of China (20306026 and 20876141) and the National Basic Research Program of China (2007CB707805).

Abstract

Abstract: 5-Aminolevulinic acid (ALA) is a common precursor for tetrapyrrole compounds in all kinds of organisms and has wide applications in agriculture and medicines. In this study, a new strategy, i.e. short-term dissolved oxygen (DO) shock during aerobic fermentation, was introduced to produce 5-aminolevulinic acid with a recombinant E. coli. Effects of duration time of DO shock operation on plasmid concentration, intracellular ALA synthase (ALAS) activity and ALA production were investigated in Erlenmeyer shake flasks. The results indicated that both ALAS activity and ALA yield were enhanced in an anaerobic operation of 45 min in the early exponential phase during fermentation, while they decreased when the anaerobic operation time was further increased to 60 min. The DO shock protocol was confirmed with the fed-batch fermentation in a 15 L fermenter and the ALA production achieved 9.4 g·L^-1 (72 mmol·L^-1), which is the highest yield in the fermentation broth reported up to now.

Key words: 5-aminolevulinic acid, synthase activity, dissolved oxygen shock, fed-batch fermentation, recombinant E. coli

摘要： 5-Aminolevulinic acid (ALA) is a common precursor for tetrapyrrole compounds in all kinds of organisms and has wide applications in agriculture and medicines. In this study, a new strategy, i.e. short-term dissolved oxygen (DO) shock during aerobic fermentation, was introduced to produce 5-aminolevulinic acid with a recombinant E. coli. Effects of duration time of DO shock operation on plasmid concentration, intracellular ALA synthase (ALAS) activity and ALA production were investigated in Erlenmeyer shake flasks. The results indicated that both ALAS activity and ALA yield were enhanced in an anaerobic operation of 45 min in the early exponential phase during fermentation, while they decreased when the anaerobic operation time was further increased to 60 min. The DO shock protocol was confirmed with the fed-batch fermentation in a 15 L fermenter and the ALA production achieved 9.4 g·L^-1 (72 mmol·L^-1), which is the highest yield in the fermentation broth reported up to now.

关键词: 5-aminolevulinic acid, synthase activity, dissolved oxygen shock, fed-batch fermentation, recombinant E. coli

YANG Jun, ZHU Li, FU Weiqi, LIN Yijun, LIN Jianping, CEN Peilin. Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation[J]. Chin.J.Chem.Eng., 2013, 21(11): 1291-1295.

杨俊, 朱力, 傅维琦, 林逸君, 林建平, 岑沛霖. Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation[J]. Chinese Journal of Chemical Engineering, 2013, 21(11): 1291-1295.

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Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

Improved 5-Aminolevulinic Acid Production with Recombinant Escherichia coli by a Short-term Dissolved Oxygen Shock in Fed-batch Fermentation

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