Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO2 foaming

doi:10.1016/j.cjche.2017.04.005

Abstract

Abstract: Scaffolds with multimodal pore structure are essential to cells differentiation and proliferation in bone tissue engineering. Bi-/multi-modal porous PLGA/hydroxyapatite composite scaffolds were prepared by supercritical CO₂ foaming in which hydroxyapatite acted as heterogeneous nucleation agent. Bimodal porous scaffolds were prepared under certain conditions, i.e. hydroxyapatite addition of 5%, depressurization rate of 0.3 MPa·min^-1, soaking temperature of 55℃, and pressure of 9 MPa. And scaffolds presented specific structure of small pores (122 μm±66 μm) in the cellular walls of large pores (552 μm±127 μm). Furthermore, multimodal porous PLGA scaffolds with micro-pores (37 μm±11 μm) were obtained at low soaking pressure of 7.5 MPa. The interconnected porosity of scaffolds ranged from (52.53±2.69)% to (83.08±2.42)% by adjusting depressurization rate, while compression modulus satisfied the requirement of bone tissue engineering. Solvent-free CO₂ foaming method is promising to fabricate bi-/multi-modal porous scaffolds in one step, and bioactive particles for osteogenesis could serve as nucleation agents.

Key words: Supercritical carbon dioxide, Foam, Tissue engineering, PLGA scaffolds, Hydroxyapatite, Bi-/multi-modal pore

摘要： Scaffolds with multimodal pore structure are essential to cells differentiation and proliferation in bone tissue engineering. Bi-/multi-modal porous PLGA/hydroxyapatite composite scaffolds were prepared by supercritical CO₂ foaming in which hydroxyapatite acted as heterogeneous nucleation agent. Bimodal porous scaffolds were prepared under certain conditions, i.e. hydroxyapatite addition of 5%, depressurization rate of 0.3 MPa·min^-1, soaking temperature of 55℃, and pressure of 9 MPa. And scaffolds presented specific structure of small pores (122 μm±66 μm) in the cellular walls of large pores (552 μm±127 μm). Furthermore, multimodal porous PLGA scaffolds with micro-pores (37 μm±11 μm) were obtained at low soaking pressure of 7.5 MPa. The interconnected porosity of scaffolds ranged from (52.53±2.69)% to (83.08±2.42)% by adjusting depressurization rate, while compression modulus satisfied the requirement of bone tissue engineering. Solvent-free CO₂ foaming method is promising to fabricate bi-/multi-modal porous scaffolds in one step, and bioactive particles for osteogenesis could serve as nucleation agents.

关键词: Supercritical carbon dioxide, Foam, Tissue engineering, PLGA scaffolds, Hydroxyapatite, Bi-/multi-modal pore

Xin Xin, Yixin Guan, Shanjing Yao. Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO₂ foaming[J]. Chin.J.Chem.Eng., 2018, 26(1): 207-212.

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Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO₂ foaming

Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO₂ foaming

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