Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K3PO4

doi:10.1016/j.cjche.2017.06.005

Abstract

Abstract: 1,3-Propanediol, traditionally obtained from fossils, has numerous industrial applications, including use in the production of high performance polymers. The microbial production of 1,3-propanediol presents several opportunities, and the final purity grade determines its price and commercial viability. The development of novel separation technology could improve the economic viability of the bioproduction of 1,3-propanediol. Thus, we investigated salting-out extraction as a novel process for 1,3-propanediol recovery from fermentation broth. Initially, a screening for the best salt/solvent combination was conducted and then optimized using the response surface methodology. The solvents studied were methanol, ethanol, isopropanol and acetone, and the salts examined were K₂HPO₄, Na₂CO₃, K₂CO₃, (NH₄)₂SO₄, NaHPO₄, K₃PO₄ and C₆H₅NaO₇. The optimal extraction system consisted of 34 wt% K₃PO₄, 28 wt% ethanol, and 38 wt% fermentation broth containing 23.0 g·L^-1 1,3-propanediol, which gave the highest partition coefficient of 33 and recovery yield of 97%. The results demonstrated that salting-out extraction was a promising method for 1,3-propanediol recovery from fermentation broth.

Key words: Fermentation, Downstream process, 1,3-Propanediol, Clostridium beijerinckii, Liquid-liquid extraction, Salting-out extraction

摘要： 1,3-Propanediol, traditionally obtained from fossils, has numerous industrial applications, including use in the production of high performance polymers. The microbial production of 1,3-propanediol presents several opportunities, and the final purity grade determines its price and commercial viability. The development of novel separation technology could improve the economic viability of the bioproduction of 1,3-propanediol. Thus, we investigated salting-out extraction as a novel process for 1,3-propanediol recovery from fermentation broth. Initially, a screening for the best salt/solvent combination was conducted and then optimized using the response surface methodology. The solvents studied were methanol, ethanol, isopropanol and acetone, and the salts examined were K₂HPO₄, Na₂CO₃, K₂CO₃, (NH₄)₂SO₄, NaHPO₄, K₃PO₄ and C₆H₅NaO₇. The optimal extraction system consisted of 34 wt% K₃PO₄, 28 wt% ethanol, and 38 wt% fermentation broth containing 23.0 g·L^-1 1,3-propanediol, which gave the highest partition coefficient of 33 and recovery yield of 97%. The results demonstrated that salting-out extraction was a promising method for 1,3-propanediol recovery from fermentation broth.

关键词: Fermentation, Downstream process, 1, 3-Propanediol, Clostridium beijerinckii, Liquid-liquid extraction, Salting-out extraction

Daiana Wischral, Hongxin Fu, Fernando L. Pellegrini Pessoa, Nei Pereira Jr, Shang-Tian Yang. Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K₃PO₄[J]. Chin.J.Chem.Eng., 2018, 26(1): 104-108.

References

[1] W. Sabra, C. Groeger, A.P. Zeng, Microbial cell factories for diol production, Adv. Biochem. Eng. Biotechnol. (2015) 165-197.

[2] J.J. Malinowski, Evaluation of liquid extraction potentials for downstream separation of 1,3-propanediol, Biotechnol. Tech. 13(1999) 127-130.

[3] D. Wischral, J. Zhang, C. Cheng, M. Lin, L.M. De Souza, F.L.P. Pessoa, N. Pereira Jr., S.-T. Yang, Production of 1, 3-propanediol by Clostridium beijerinckii DSM 791 from crude glycerol and corn steep liquor:Process optimization and metabolic engineering, Bioresour. Technol. 212(2016) 100-110.

[4] W.D. Deckwer, Microbial conversion of glycerol production to 1,3-propanediol, FEMS Microbiol. Rev. 16(1995) 143-149.

[5] B.G. Hermann, M. Patel, Today's and tomorrow's bio-based bulk chemicals from white biotechnology:A techno-economic analysis, Appl. Biochem. Biotechnol. 136(2007) 361-388.

[6] Z.L. Xiu, A.P. Zeng, Present state and perspective of downstream processing of biologically produced 1,3-propanediol and 2,3-butanediol, Appl. Microbiol. Biotechnol. 78(2008) 917-926.

[7] P. Anand, R.K. Saxena, R.G. Marwah, A novel downstream process for 1, 3-propanediol from glycerol-based fermentation, Appl. Microbiol. Biotechnol. 90(2011) 1267-1276.

[8] Z.L. Xiu, Z. Li, B. Jianng, Y. Sun, D. Zhang, Aqueous two-phase extraction of 1,3-propanediol from fermentation broth, China Pat, 200710010201.X, 2007.

[9] R. Saxena, P. Anand, S. Saran, J. Isar, Microbial production of 1,3-propanediol:Recent developments and emerging opportunities, Biotechnol. Adv. 27(2009) 895-913.

[10] A. Greve, M.R. Kula, Cost structure and estimation for the recycling of salt in a protein extraction process system, Bioprocess Eng. 6(1990) 173-177.

[11] A. Louwrier, Model phase separations of proteins using aqueous/ethanol components, Biotechnol. Tech. 12(1998) 363-365.

[12] B. Jiang, Z.G. Li, J.Y. Dai, D.J. Zhang, Aqueous two phase extraction of 2,3-butanediol from fermentation broths using an ethanol/phosphate system, Process Biochem. 44(2009) 112-117.

[13] Z. Li, B. Jiang, D. Zhang, Z. Xiu, Aqueous two-phase extraction of 1,3-propanediol from glycerol-based fermentation broths, Sep. Purif. Technol. 66(2009) 472-478.

[14] J.N. Sun, B. Rao, L.Y. Zhang, Y.L. Shen, Extraction of acetoin from fermentation broth using an acetone/phosphate aqueous two-phase system, Chem. Eng. Commun. 199(2012) 1492-1503.

[15] D. Wischral, C.A. Barcelos, N. Pereira Jr., F.L.P. Pessoa, 1,3-Propanediol:Statistical optimization of medium to improve production by Clostridium beijerinckii DSM 791, J. Adv. Biotechnol. 5(2015) 614-623.

[16] O. Aydogan, E. Bayraktar, U. Mehmetoglu, T. Kaeding, Selection and optimization of an aqueous two-phase system for the recovery of 1,3-propandiol from fermentation broth, Eng. Life Sci. 10(2010) 121-129.

[17] J.Y. Dai, Y.L. Zhang, Z.L. Xiu, Salting-out extraction of 2,3-butanediol from Jerusalem artichoke-based fermentation broth, Chin. J. Chem. Eng. 19(2011) 682-686.

[18] Z.G. Li, H. Teng, Z.L. Xiu, Extraction of 1,3-propanediol from glycerol-based fermentation broths with methanol/phosphate aqueous two-phase system, Process Biochem. 46(2011) 586-591.

[19] Y. Sun, L. Yan, H. Fu, Z. Xiu, Selection and optimization of a salting-out extraction system for recovery of biobutanol from fermentation broth, Eng. Life Sci. 13(2013) 464-471.

[20] H. Fu, S.-T. Yang, Z. Xiu, Phase separation in a salting-out extraction system of ethanol-ammonium sulphate, Sep. Purif. Technol. 148(2015) 32-37.

[21] S. Shahriari, C.M.S.S. Neves, M.G. Freire, J.A.P. Coutinho, Role of the Hofmeister series in the formation of ionic-liquid-based aqueous biphasic systems, J. Phys. Chem. 116(2012) 7252-7258.

[22] H. Fu, Y. Sun, Z. Xiu, Continuous countercurrent salting-out extraction of 1,3-propanediol from fermentation broth in a packed column, Process Biochem. 48(2013) 1381-1386.

[23] Z.G. Li, Y.Q. Sun, W.L. Zheng, H. Teng, Z.L. Xiu, A novel and environment-friendly bioprocess of 1, 3-propanediol fermentation integrated with aqueous two-phase extraction by ethanol/sodium carbonate system, Biochem. Eng. J. 80(2013) 68-75.

[24] C. Xue, J. Zhao, L. Chen, S.T. Yang, F. Bai, Recent advances and state-of-the-art strategies in strain and process engineering for biobutanol production by Clostridium acetobutylicum, Biotechnol. Adv. 35(2017) 310-322.

[25] C. Xue, F. Liu, M. Xu, J. Zhao, L. Chen, J. Ren, F. Bai, S.T. Yang, A novel in situ gas stripping-pervaporation process integrated with acetone-butanol-ethanol fermentation for hyper n-butanol production, Biotechnol. Bioeng. 113(2016) 120-129.

[26] C. Xue, M. Liu, X. Guo, E.P. Hudson, L. Chen, F. Bai, F. Liu, S.T. Yang, Bridging chemicaland bio-catalysis:High-value liquid transportation fuel production from renewable agricultural residues, Green Chem. 19(2017) 660-669.

[27] H. Fu, Y. Sun, Hu Teng, D. Zhang, Z.L. Xiu, Salting-out extraction of carboxylic acids, Sep. Sci. Technol. 139(2015) 36-42.

[28] H. Fu, J. Dai, Y. Sun, D. Zhang, Z.L. Xiu, Partition behavior of hydrophilic diols in an ethanol/ammonium sulfate salting-out extraction system, Eng. Life Sci. 15(2016) 797-803.

[29] O. Aydogan, E. Bayraktar, U. Mehmetoglu, Aqueous two-phase extraction of lactic acid:Optimization by response surface methodology, Sep. Sci. Technol. 46(2011) 1164-1171.

[30] B.C. Wei, Z.Y. Song, Y.Q. Sun, Z.L. Xiu, Salting-out extraction of lactic acid from fermentation broths, Chin. J. Chem. Eng. 12(2012) 44-48.

[31] H. Fu, X. Wang, Y. Sun, L. Yan, J. Shen, J. Wang, S.T. Yang, Z.L. Xiu, Effects of saltingout and salting-out extraction on the separation of butyric acid, Sep. Sci. Technol. 180(2017) 44-50.

Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K₃PO₄

Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid-liquid extraction system with ethanol and K₃PO₄

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Tutuk Djoko Kusworo, Monica Yulfarida, Andri Cahyo Kumoro, Dani Puji Utomo. Purification of bioethanol fermentation broth using hydrophilic PVA crosslinked PVDF-GO/TiO₂ membrane [J]. Chinese Journal of Chemical Engineering, 2023, 55(3): 123-136.
[2]	Xinyu Yan, Bobo Wang, Hongxia Liang, Jie Yang, Jie Zhao, Fabrice Ndayisenga, Hongxun Zhang, Zhisheng Yu, Zhi Qian. Enhanced straw fermentation process based on microbial electrolysis cell coupled anaerobic digestion [J]. Chinese Journal of Chemical Engineering, 2022, 44(4): 239-245.
[3]	Xiaocui Sun, Xue Liu, Guang-Rong Zhao. Separation of salidroside from the fermentation broth of engineered Escherichia coli using macroporous adsorbent resins [J]. Chinese Journal of Chemical Engineering, 2022, 44(4): 260-267.
[4]	Min Wu, Juanjuan Ding, Zhaofeng Zhang, Shengping You, Wei Qi, Rongxin Su, Zhimin He. Kinetic modeling of gamma-aminobutyric acid production by Lactobacillus brevis based on pH-dependent model and rolling correction [J]. Chinese Journal of Chemical Engineering, 2022, 50(10): 352-360.
[5]	Zhaofeng Zhang, Juanjuan Ding, Min Wu, Bochao Liu, Huiwen Song, Shengping You, Wei Qi, Rongxin Su, Zhimin He. Development of an integrated process for the production of high-purity γ-aminobutyric acid from fermentation broth [J]. Chinese Journal of Chemical Engineering, 2022, 50(10): 361-368.
[6]	Youming Jiang, Xiaohan Ye, Tianwen Zheng, Weiliang Dong, Fengxue Xin, Jiangfeng Ma, Min Jiang. Microbial production of L-malate from renewable non-food feedstocks [J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 105-111.
[7]	Jianye Xia, Guan Wang, Meng Fan, Min Chen, Zeyu Wang, Yingping Zhuang. Understanding the scale-up of fermentation processes from the viewpoint of the flow field in bioreactors and the physiological response of strains [J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 178-184.
[8]	Jianying Dai, Yaqin Sun, Zhilong Xiu. Ionic liquid-based salting-out extraction of bio-chemicals [J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 185-193.
[9]	Bahare Esmaeeli, Ahad Ghaemi, Mansour Shirvani, Mostafa Hosseinzadeh. Mass transfer coefficient in the eductor liquid-liquid extraction column [J]. Chinese Journal of Chemical Engineering, 2021, 40(12): 27-35.
[10]	Huiyu Li, Ming Gao, Pan Wang, Hongzhi Ma, Ting Liu, Jin Ni, Qunhui Wang, Tien-Chin Chang. Cathode catalyst prepared from bacterial cellulose for ethanol fermentation stillage treatment in microbial fuel cell [J]. Chinese Journal of Chemical Engineering, 2021, 40(12): 256-261.
[11]	Lijuan Zhang, Peng Hu, Jiang Pan, Huilei Yu, Jianhe Xu. Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation [J]. Chinese Journal of Chemical Engineering, 2021, 29(1): 311-316.
[12]	Qianxia Chen, Xianghong Lu, Jianbing Ji. Application of silver-based dihydric alcohol to the extraction of methyl linolenate with high extractability and stability replacing ionic liquids [J]. Chinese Journal of Chemical Engineering, 2020, 28(3): 871-880.
[13]	Inn Shi Tan, Man Kee Lam, Henry Chee Yew Foo, Steven Lim, Keat Teong Lee. Advances of macroalgae biomass for the third generation of bioethanol production [J]. Chinese Journal of Chemical Engineering, 2020, 28(2): 502-517.
[14]	Niphaphat Phukoetphim, Naulchan Khongsay, Pattana Laopaiboon, Lakkana Laopaiboon. A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice [J]. Chinese Journal of Chemical Engineering, 2019, 27(7): 1651-1658.
[15]	Senqing Fan, Jingyun Liu, Xiaoyu Tang, Wenguo Wang, Zeyi Xiao, Boya Qiu, Yuyang Wang, Shizhao Jian, Yangmei Qin, Yinan Wang. Process operation performance of PDMS membrane pervaporation coupled with fermentation for efficient bioethanol production [J]. Chinese Journal of Chemical Engineering, 2019, 27(6): 1339-1347.