Glycopolymer N-halamine-modified biochars with high specificity for Escherichia coli eradication

doi:10.1016/j.cjche.2021.04.010

Chinese Journal of Chemical Engineering ›› 2021, Vol. 38 ›› Issue (10): 229-236.DOI: 10.1016/j.cjche.2021.04.010

• Biotechnology and Bioengineering • Previous Articles Next Articles

Glycopolymer N-halamine-modified biochars with high specificity for Escherichia coli eradication

Qinggele Borjihan^1,2, Quanfu Yao^3,4, Huihui Qu³, Haixia Wu^1,2, Ying Liu³, Alideertu Dong^1,2

1. College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China;
2. Engineering Research Center of Dairy Quality and Safety Control Technology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China;
3. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China;
4. College of Chemistry and Environment, Hohhot Minzu College, Hohhot 010051, China

Received:2021-02-26 Revised:2021-04-23 Online:2021-12-02 Published:2021-10-28
Contact: Haixia Wu, Ying Liu, Alideertu Dong
Supported by:
This work was supported by the National Natural Science Foundation of China (21304044, 51663019, and 22062017), the Natural Science Foundation of Inner Mongolia Autonomous Region (2015MS0520, 2019JQ03 and 2019BS02004), the State Key Laboratory of Medicinal Chemical Biology (201603006 and 2018051), the State Key Laboratory of Polymer Physics and Chemistry (2018-08), and the Program of Higher-Level Talents of Inner Mongolia University (30105-125136)

Glycopolymer N-halamine-modified biochars with high specificity for Escherichia coli eradication

Qinggele Borjihan^1,2, Quanfu Yao^3,4, Huihui Qu³, Haixia Wu^1,2, Ying Liu³, Alideertu Dong^1,2

1. College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China;
2. Engineering Research Center of Dairy Quality and Safety Control Technology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China;
3. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China;
4. College of Chemistry and Environment, Hohhot Minzu College, Hohhot 010051, China

通讯作者: Haixia Wu, Ying Liu, Alideertu Dong
基金资助:
This work was supported by the National Natural Science Foundation of China (21304044, 51663019, and 22062017), the Natural Science Foundation of Inner Mongolia Autonomous Region (2015MS0520, 2019JQ03 and 2019BS02004), the State Key Laboratory of Medicinal Chemical Biology (201603006 and 2018051), the State Key Laboratory of Polymer Physics and Chemistry (2018-08), and the Program of Higher-Level Talents of Inner Mongolia University (30105-125136)

Abstract

Abstract: Antibiotic-resistant bacteria contamination in environments imposes great threats to human life health. This research aims to develop novel targeted antibacterial biochars for achieving high selectivity to kill pathogenic Escherichia coli (E. coli). The glycopolymer N-halamine-modified biochars (i.e., BCPMA-Cl) were synthesized by the modification of biochars with poly[2-(methacrylamido) glucopyranose-co-acrylamide] (P(MAG-co-AM), followed by chlorination treatment. Based on the results of FTIR, turbidity, XPS, and UV-vis, BCPMA-Cl was successfully synthesized and demonstrated to be able to eliminate Staphylococcus aureus (S. aureus) and E. coli. Especially, BCPMA-Cl possessed extremely potent to specific-killing 10⁴ CFU·ml^-1 of E. coli with lower hemolytic activity (<5%). Additionally, the antibacterial mechanisms of BCPMA-Cl against bacteria were contact-killing and release-killing contributed by active chlorine (i.e., Cl⁺). Therefore, this work provided a cost-effective and facile approach for preparation of functional biochars used for bacteria-specific therapeutic applications via livestock pollutants as well as showing a promising strategy to avoid bacterial resistance.

Key words: Biochars, N-halamine, Glycopolymer, Antibacterial coating, Specific killing

摘要： Antibiotic-resistant bacteria contamination in environments imposes great threats to human life health. This research aims to develop novel targeted antibacterial biochars for achieving high selectivity to kill pathogenic Escherichia coli (E. coli). The glycopolymer N-halamine-modified biochars (i.e., BCPMA-Cl) were synthesized by the modification of biochars with poly[2-(methacrylamido) glucopyranose-co-acrylamide] (P(MAG-co-AM), followed by chlorination treatment. Based on the results of FTIR, turbidity, XPS, and UV-vis, BCPMA-Cl was successfully synthesized and demonstrated to be able to eliminate Staphylococcus aureus (S. aureus) and E. coli. Especially, BCPMA-Cl possessed extremely potent to specific-killing 10⁴ CFU·ml^-1 of E. coli with lower hemolytic activity (<5%). Additionally, the antibacterial mechanisms of BCPMA-Cl against bacteria were contact-killing and release-killing contributed by active chlorine (i.e., Cl⁺). Therefore, this work provided a cost-effective and facile approach for preparation of functional biochars used for bacteria-specific therapeutic applications via livestock pollutants as well as showing a promising strategy to avoid bacterial resistance.

关键词: Biochars, N-halamine, Glycopolymer, Antibacterial coating, Specific killing

Qinggele Borjihan, Quanfu Yao, Huihui Qu, Haixia Wu, Ying Liu, Alideertu Dong. Glycopolymer N-halamine-modified biochars with high specificity for Escherichia coli eradication[J]. Chinese Journal of Chemical Engineering, 2021, 38(10): 229-236.

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Glycopolymer N-halamine-modified biochars with high specificity for Escherichia coli eradication

Glycopolymer N-halamine-modified biochars with high specificity for Escherichia coli eradication

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