Investigates of substrate mingling ratio and organic loading rate of KOH pretreated corn stover and pig manure in batch and semi-continuous system: Anaerobic digestion performance and microbial characteristics

doi:10.1016/j.cjche.2023.04.014

Abstract

Abstract: The effects of substrate mingling ratio (SMR) (1:1, 1:2, 1:3, 3:1, and 2:1) and organic loading rate (OLR) (50–90 g total solids per liter per day) on anaerobic co-digestion performance and microbial characteristics were investigated for pig manure (PM) and pretreated/untreated corn stover in batch and semi-continuous anaerobic digestion (AD) system. The results showed that SMR and pretreatment affected co-digestion performance. The maximum cumulative methane yield of 428.5 ml·g^-1 (based on volatile solids (VS)) was obtained for PC_P13, which was 35.7% and 40.0% higher than that of CS_U and PM. In the first 5 days, the maximum methane yield improvement rate was 378.1% for PC_P13. The daily methane yield per gram VS of PC_P13 was 11.4%–18.5% higher than that of PC_U13. Clostridium_sensu_stricto_1, DMER64, and Bacteroides and Methanosaeta, Methanobacterium, and Methanospirillum had higher relative abundance at the genus level. Therefore, SMR and OLR are important factor affecting the AD process, and OLR can affect methane production through volatile fatty acids.

Key words: Substrate mingling ratio, Organic loading rate, Co-digestion, Corn stover, Pig manure, Microbial community

摘要： The effects of substrate mingling ratio (SMR) (1:1, 1:2, 1:3, 3:1, and 2:1) and organic loading rate (OLR) (50–90 g total solids per liter per day) on anaerobic co-digestion performance and microbial characteristics were investigated for pig manure (PM) and pretreated/untreated corn stover in batch and semi-continuous anaerobic digestion (AD) system. The results showed that SMR and pretreatment affected co-digestion performance. The maximum cumulative methane yield of 428.5 ml·g^-1 (based on volatile solids (VS)) was obtained for PC_P13, which was 35.7% and 40.0% higher than that of CS_U and PM. In the first 5 days, the maximum methane yield improvement rate was 378.1% for PC_P13. The daily methane yield per gram VS of PC_P13 was 11.4%–18.5% higher than that of PC_U13. Clostridium_sensu_stricto_1, DMER64, and Bacteroides and Methanosaeta, Methanobacterium, and Methanospirillum had higher relative abundance at the genus level. Therefore, SMR and OLR are important factor affecting the AD process, and OLR can affect methane production through volatile fatty acids.

关键词: Substrate mingling ratio, Organic loading rate, Co-digestion, Corn stover, Pig manure, Microbial community

Chenyang Zhu, Ruoran Qu, Xiujin Li, Xiaoyu Zuo, Hairong Yuan. Investigates of substrate mingling ratio and organic loading rate of KOH pretreated corn stover and pig manure in batch and semi-continuous system: Anaerobic digestion performance and microbial characteristics[J]. Chinese Journal of Chemical Engineering, 2023, 62(10): 114-123.

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