Abstract: Two dense pellicular agarose-glass matrices of different sizes and densities, i.e., AG-S
and AG-L, have been characterized for their bed expansion behavior, flow hydrodynamics and
particle classifications in an expanded bed system. A 26 mm ID column with side ports was
used for sampling the liquid-solidsuspension during expanded bed operations. Measurements
of the collected solid phase at different column positions yielded the particle size and
density distribution data. It was found that the composite matrices showed particle size as
well as density classifications along the column axis, i.e., both the size and density of
each matrix decreased with increasing the axial bed height. Their axial classifications
were expressed by a correlation related to both the particle size and density as a function
of the dimensionless axial bed height. The correlation was found to fairly describe the
solid phase classifications in the expanded bed system. Moreover, it can also be applied to
other two commercial solid matrices designed for expanded bed applications.

Key words: expanded bed, dense pellicular medium, solid phase classification, size distribution, density distribution

摘要： Two dense pellicular agarose-glass matrices of different sizes and densities, i.e., AG-S
and AG-L, have been characterized for their bed expansion behavior, flow hydrodynamics and
particle classifications in an expanded bed system. A 26 mm ID column with side ports was
used for sampling the liquid-solidsuspension during expanded bed operations. Measurements
of the collected solid phase at different column positions yielded the particle size and
density distribution data. It was found that the composite matrices showed particle size as
well as density classifications along the column axis, i.e., both the size and density of
each matrix decreased with increasing the axial bed height. Their axial classifications
were expressed by a correlation related to both the particle size and density as a function
of the dimensionless axial bed height. The correlation was found to fairly describe the
solid phase classifications in the expanded bed system. Moreover, it can also be applied to
other two commercial solid matrices designed for expanded bed applications.

关键词: 琼脂糖;玻璃珠;膨胀床;流体;固体