Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride

Abstract

Abstract: One of the bottlenecks for bioproduction of butyric acid as bulk chemical is the difficulty in separating butyric acid from the fermentation broth,compared with the petroleum-based chemical synthesis method.In the present work,a novel separation methodology was developed based on an aqueous two-phase system with inor-ganic salts.Calcium chloride was screened out for effective separation of butyric acid from butyric acid-water-salt systems.Within appropriate concentration range of butyric acid and salt,butyric acid was enriched in the upper phase and most of calcium ions remained in the lower phase.This"salting out"effect is very efficient to separate butyric acid from the simulated butyrate fermentation broth,which consists of butyric acid and acetic acid with concentration ratio of 4:1,so that the final ratio of butyric acid/acetic acid in the upper phase is improved to 9.87. The aqueous two-phase system was used to separate butyric acid from the actual fermentation broth with satisfactory result.

Key words: butyric acid, separation, aqueous two-phase partition, salting out effect, calcium chloride

摘要： One of the bottlenecks for bioproduction of butyric acid as bulk chemical is the difficulty in separating butyric acid from the fermentation broth,compared with the petroleum-based chemical synthesis method.In the present work,a novel separation methodology was developed based on an aqueous two-phase system with inor-ganic salts.Calcium chloride was screened out for effective separation of butyric acid from butyric acid-water-salt systems.Within appropriate concentration range of butyric acid and salt,butyric acid was enriched in the upper phase and most of calcium ions remained in the lower phase.This"salting out"effect is very efficient to separate butyric acid from the simulated butyrate fermentation broth,which consists of butyric acid and acetic acid with concentration ratio of 4:1,so that the final ratio of butyric acid/acetic acid in the upper phase is improved to 9.87. The aqueous two-phase system was used to separate butyric acid from the actual fermentation broth with satisfactory result.

关键词: butyric acid, separation, aqueous two-phase partition, salting out effect, calcium chloride

WU Dan, CHEN Hao, JIANG Ling, CAI Jin, XU Zhinan, CEN Peilin. Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride[J]. , 2010, 18(4): 533-537.

吴丹, 陈灏, 江凌, 蔡谨, 徐志南, 岑沛霖. Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride[J]. , 2010, 18(4): 533-537.

References

1 Kuroiwa-Trzmielina,J.,De Conti,A.,Scolastici,C.,Pereira,D.,Horst,M.A.,Purgatto,E.,Ong,T.P.,Moreno,F. S.,“Chemoprevention of rat hepatocarcinogenesis with histone deacetylase inhibitors:Efficacy of tributyrin,a butyric acid prodrug”,Int.J.Cancer,124(11),2520-2527(2009).
2 Geffen,Y.,Nudelman,A.,Gil-Ad,I.,Rephaeli,A.,Huang,M.,Savitsky,K.,Klapper,L.,Winkler,I.,Meltzer,H. Y.,Weizman,A.,“BL-1020:A novel antipsychotic drug with GABAergic activity and low catalepsy,is efficacious in a rat model of schizophrenia”,Eur. Neuropsychopharmacol.,19(1),1-13(2009).
3 Blank-Porat,D.,Gruss-Fischer,T.,Tarasenko,N.,Malik,Z.,Nudelman,A.,Rephaeli,A.,“The anticancer prodrugs of butyric acid AN-7 and AN-9,possess antiangiogenic properties”,Cancer Lett.(Amsterdam,Neth.),256(1),39-48(2007).
4 Rephaeli,A.,Entin-Meer,M.,Angel,D.,Tarasenko,N.,Gruss-Fischer,T.,Bruachman,I.,Phillips,D.R.,Cutts,S.M.,Haas-Kogan,D.,Nudelman,A.,“The selectivity and anti-metastatic activity of oral bioavailable butyric acid prodrugs”,Invest.New Drugs,24(5),383-392(2006).
5 Entin-Meer,M.,Rephaeli,A.,Yang,X.,Nudelman,A.,Vandenberg,S.R.,Haas-Kogan,D.A.,“Butyric acid prodrugs are histone deacetylase inhibitors that show antineoplastic activity and radiosensitizing capacity in the treatment of malignant gliomas”,Mol.Cancer Ther.,4(12),1952-1961(2005).
6 Rephaeli,A.,Zhuk,R.,Nudelman,A.,“Prodrugs of butyric acid from bench to bedside:synthetic design,mechanisms of action,and clinical applications”,Drug Dev.Res.,50(3/4),379-391(2000).
7 Aviram,A.,Rephaeli,A.,Shaklai,M.,Nudelmann,A.,Ben-Dror,I.,Maron,L.,Rabizadeh,E.,“Effect of the cytostatic butyric acid pro-drug,pivaloyloxymethyl butyrate,on the tumorigenicity of cancer cells”,J.Cancer Res.Clin.Oncol.,123(5),267-271(1997).
8 Bilgin,M.,Kirbaslar,S.I.,Oezcan,O.,Dramur,U.,“Distribution of butyric acid between water and several solvents”,J.Chem.Eng.Data,51
(5),1546-1550(2006).
9 Huang,Y.L.,Wu,Z.,Zhang,L.,Ming,C.C.,Yang,S.T.,“Production of carboxylic acids from hydrolyzed corn meal by immobilized cell fermentation in a fibrous-bed bioreactor”,Bioresour.Technol.,82(1),51-59(2002).
10 Playne,M.J.,“Propionic and butyric acids”,In:Comprehensive Biotechnology,Pergamon Press,Oxford,731-759(1985).
11 Zhu,Y.,“Enhanced butyric acid fermentation by clostridium tyrobu-tyricum immobilized in a fibrous-bed bioreactor”,Ph.D.Thesis,Ohio(2003).
12 Wang,G. H.,Feng,X.L.,Su,Z. G.,“Simultaneous cell disruption and aqueous two-phase extraction for isolation of intracellular recombinant proteins”,Chin.J.Chem.Eng.,7(2),139-144(1999).
13 Zhu,Z. Q.,Li,M.,Mei,L.H.,Han,Z.X.,“Measurement and correlation of partition coefficients of urokinase in aqueous two-phase PEG-potassium phosphate system”,Chin. J. Chem.Eng.,4(1),19-27(1996).
14 Martin,J.,Krchma,I. J.,“Method of separating and recovering acetic and butyric acids”,U.S.Pat.,1917660(1933).
15 Cherkasov,D. G.,Il'in,K.K.,“Salting out of butyric acid from aqueous solutions with potassium chloride”,Russ.J.Appl.Chem.,82(5),920-924(2009).16 Jiang,L.,Wang,J. F.,Liang,S. Z.,Wang,X. N.,Cen,P. L.,Xu,Z. N.,“Butyric acid fermentation in a fibrous bed bioreactor with immobilized Clostridium tyrobutyricum from cane molasses”,Bioresour.Technol.,100(13),3403-3409(2009).
17 Tianjin Institute of Light Industry,Dalian Institute of Light Industry,Wuxi Institute of Light Industry,South China Institute of Technology,Industrial Fermentation Analysis,Light Publishing Press,Beijing(1979).
18 Tansel,B.,Sager,J.,Rector,T.,Garland,J.,Strayer,R.F.,Levine,L.F.,Roberts,M.,Hummerick,M.,Bauer,J.,“Significance of hydrated radius and hydration shells on ionic permeability during nanofiltration in dead end and cross flow modes”,Sep.Purif.Technol.,51(1),40-47(2006).
19 Madaeni,S. S.,Kazemi,V.,“Treatment of saturated brine in chlor-alkali process using membranes”,Sep.Purif.Technol.,61(1),68-74(2008).

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