Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920*

Chin.J.Chem.Eng. ›› 2012, Vol. 20 ›› Issue (5): 930-936.

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*

YANG Chanyuan^1,2, ZHU Xiaoli^1,3, FAN Daidi^1,2, MI Yu^1,2, LUO Yan'e^1,2, HUI Junfeng^1,2, SU Ran^1,2

1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
2. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
3. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University, Xi'an 710069, China

Received:2011-03-06 Revised:2011-09-29 Online:2012-11-06 Published:2012-10-28
Supported by:
Supported by the National High Technology Research and Development Program of China (2007AA03Z456);the National Natural Science Foundation of China (20776119, 21076169 and 31000019);the Xi’an Research and Development Program(NC08005, YF07078);the Scientific Research Program of Shaanxi Provincial Department of Education,China(08JK452,08JK453,JG08181,2010JC21,2010JS107,2010JS108, 2010JK876 and 2010JS109);Shaanxi Provincial Scientific Technology Research and Development Program (2007K06-03, 2010JQ2012, SJ08B03);the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014);NWU Graduate Innovation and Creativity Funds (08YSY17);Shaanxi Key Subject Program, China

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*

杨婵媛^1,2, 朱晓丽^1,3, 范代娣^1,2, 米钰^1,2, 骆艳娥^1,2, 惠俊峰^1,2, 苏然^1,2

1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
2. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
3. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University, Xi'an 710069, China

通讯作者: FAN Daidi,E-mail:fandaidi@nwu.edu.cn;MI Yu,E-mail:mi_yu@nwu.edu.cn
基金资助:
Supported by the National High Technology Research and Development Program of China (2007AA03Z456);the National Natural Science Foundation of China (20776119, 21076169 and 31000019);the Xi’an Research and Development Program(NC08005, YF07078);the Scientific Research Program of Shaanxi Provincial Department of Education,China(08JK452,08JK453,JG08181,2010JC21,2010JS107,2010JS108, 2010JK876 and 2010JS109);Shaanxi Provincial Scientific Technology Research and Development Program (2007K06-03, 2010JQ2012, SJ08B03);the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014);NWU Graduate Innovation and Creativity Funds (08YSY17);Shaanxi Key Subject Program, China

Abstract

Abstract: Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the comparative analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×10⁹ cfu·g^-1, greatly exceeding the minimum viable count requirement (10⁶cfu·g^-1).

Key words: Bifidobacterium, freeze-drying, viability, protective agent, response surface methodology

摘要： Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the comparative analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×10⁹ cfu·g^-1, greatly exceeding the minimum viable count requirement (10⁶cfu·g^-1).

关键词: Bifidobacterium, freeze-drying, viability, protective agent, response surface methodology

YANG Chanyuan, ZHU Xiaoli, FAN Daidi, MI Yu, LUO Yan'e, HUI Junfeng, SU Ran. Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*[J]. Chin.J.Chem.Eng., 2012, 20(5): 930-936.

杨婵媛, 朱晓丽, 范代娣, 米钰, 骆艳娥, 惠俊峰, 苏然. Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 930-936.

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Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*

Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920^*

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