Supercritical Antisolvent-based Technology for Preparation of Vitamin D3 Proliposome and Its Characteristics

›› 2011, Vol. 19 ›› Issue (6): 1039-1046.

• PRODUCT ENGINEERING AND CHEMICAL TECHNOLOGY • Previous Articles Next Articles

Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics

XIA Fei¹, JIN Heyang¹, ZHAO Yaping¹, GUO Xinqiu²

1. College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
2. Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China

Received:2010-12-07 Revised:2011-07-28 Online:2012-04-24 Published:2011-12-28
Supported by:
Supported by the National High Technology Research and Development Program of China (2007AA10Z350);the National Natural Science Foundation of China (20976103)

Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics

夏菲¹, 金鹤阳¹, 赵亚平¹, 郭新秋²

1. College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
2. Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China

通讯作者: ZHAO Yaping, E-mail: ypzhao@sjtu.edu.cn
基金资助:
Supported by the National High Technology Research and Development Program of China (2007AA10Z350);the National Natural Science Foundation of China (20976103)

Abstract

Abstract: Vitamin D₃ (VD₃) proliposomes (VDP), consisted of hydrogenated phosphatidycholine (HPC) and VD₃, were prepared using supercritical anti-solvent technology (SAS). The effects of operation conditions (temperature, pressure and components) on the VD₃ loading in VDP were studied. At the optimum conditions of pressure of 8.0 MPa, temperature of 45℃, and the mass ratio of 15.0% between VD₃ and HPC, the VD₃ loading reached 12.89%. VD₃ liposomes (VDL) were obtained by hydrating VDP and the entrapment efficiency of VD₃ in VDL reached 98.5%. The morphology and structure of VDP and VDL were characterized by SEM (scanning electron microscope), TEM (transmission electron microscope) and XRD (X-ray diffractometer). The structure of VD₃ nanoparticles in HPC matrix was formed. The size of VDL with an average diameter of about 1μm was determined by dynamic light scattering instrument (DLS). The results indicated that VDP can be made by SAS and VDL with high entrapment efficiency can be formed easily via the hydration of VDP.

Key words: hydrogenated phosphatidycholine, liposome, encapsulation, supercritical anti-solvent

摘要： Vitamin D₃ (VD₃) proliposomes (VDP), consisted of hydrogenated phosphatidycholine (HPC) and VD₃, were prepared using supercritical anti-solvent technology (SAS). The effects of operation conditions (temperature, pressure and components) on the VD₃ loading in VDP were studied. At the optimum conditions of pressure of 8.0 MPa, temperature of 45℃, and the mass ratio of 15.0% between VD₃ and HPC, the VD₃ loading reached 12.89%. VD₃ liposomes (VDL) were obtained by hydrating VDP and the entrapment efficiency of VD₃ in VDL reached 98.5%. The morphology and structure of VDP and VDL were characterized by SEM (scanning electron microscope), TEM (transmission electron microscope) and XRD (X-ray diffractometer). The structure of VD₃ nanoparticles in HPC matrix was formed. The size of VDL with an average diameter of about 1μm was determined by dynamic light scattering instrument (DLS). The results indicated that VDP can be made by SAS and VDL with high entrapment efficiency can be formed easily via the hydration of VDP.

关键词: hydrogenated phosphatidycholine, liposome, encapsulation, supercritical anti-solvent

XIA Fei, JIN Heyang, ZHAO Yaping, GUO Xinqiu. Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics[J]. , 2011, 19(6): 1039-1046.

夏菲, 金鹤阳, 赵亚平, 郭新秋. Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics[J]. , 2011, 19(6): 1039-1046.

References

1 Grant, W.B., Holick, M.F., “Benefits and requirements of vitamin D for optimal health: a review”, Alternative Medicine Review, 10(2), 94-111(2005).
2 Perales, S., Alegría, A., Barberá, R., Farré, R., “Review: determination of vitamin d in dairy products by high performance liquid chromatography”, Food Sci. Tech. Int., 11(6), 451-462(2005).
3 Alien, A., Goigoux, C., Baquey, C., Dupuy, B., “Study of in vitro and in vivo stability of liposomes loaded with calcitonin or indium in the gastrointestinal tract”, Life Sci., 53, 1279-1290(1993).
4 Arien, A., Dupuy, B., “Encapsulation of calcitonin in liposomes depends in the vesicle preparation method”, J. Microencapsul., 14, 753-760(1997).
5 Bangham, A.D., Standish, M.M., Watkins, J.C., “Diffusion of univalent ions across the lamellae of swollen phospholipids”, J. Mol. Biol., 13, 238-252(1965).
6 Batzri, S., Korn, E.D., “Single bilayer liposomes prepared without sonication”, Biochim. Biophys. Acta, 298, 1015-1019(1973).
7 Deamer, D., Bangham, A.D., “Large volume liposomes by an ether vaporization method”, Biochi. Biophys. Acta, 443, 629-634(1976).
8 Watwe, M., Bellare, J.R., “Manufacture of liposomes: a review”, Curr. Sci., 68, 715-724(1995).
9 Meure, L.A., Knott, R., Foster, N.R., Dehghani, F., “The depressurization of an expanded solution into aqueous media for the bulk production of liposomes”, Langmuir, 25, 326-337(2009).
10 Xiao, Y.Y., Song, Y.M., Peng, C.Z., Ping, Q.N., “Preparation of silymarin proliposome: A new way to increase oral bioavailability of silymarin in beagle dogs”, Int. J. Pharm., 319, 162-168(2006).
11 Mayer, L.D., Baly, M.B., Hope, K.J., Cullis, P.R., “Techniques for encapsulating bioactive agents into liposomes”, Chem. Phys. Lipids, 40, 333-345(1986).
12 Katare, O.P., Vyas, S.P., Dixit, V.K., “Preparation and performance evaluation of plain proliposomal systems for cytoprotection”, J. Microencapsul., 8(3), 295-300(1991).
13 Byung-Nak, A., Shin-Keun, K., “Proliposomes as an intranasal dosage form for the sustained delivery of propranolol”, J. Control Release, 34, 203-210(1995).
14 Zhang, J.H., Zhu, J.B., “A novel method to prepare liposomes containing amikacin”, J. Microencapsul., 16, 511-516(1999).
15 Yang, Z.J., Hino, T., Kawashima, Y., “Studies on the size of rehydrated new liposome from scutellaria proliposome”, J. Chin. Pharm. Univ., 24, 161-164(1993).(in Chinese)
16 Chen, Q.I., Huang, Y.U., Gu, X.Q., Chen, C.Z., Huang, D.N., “A study on the preparation of proliposome by the spray drying method”, J. Shenyang Pharm. Univ., 14, 166-169(1997).(in Chinese)
17 Ye, Z.W., Liang, W.Q., “Preparation of interferon-containing liposomes by the powder bed grinding method”, J. Zhejiang Univ., 31, 433-436(2002).(in Chinese)
18 Wei, N.N., Lu, B.I., “Preparation, morphology and in vitro release of chitosan coated liposomes of fluorouracil for colon targeting”, Acta Pharm. Sinica, 38, 53-56(2003).
19 Xu, H.T., He, L., Nie, S.F., Guan, J., Zhang, X.N., Yang, X.G., Pan, W.S., “Optimized preparation of vinpocetine proliposomes by a novel method and in vivo evaluation of its pharmacokinetics in New Zealand rabbits”, J. Control Release, 140, 61-68(2009).
20 Jin, H.Y., Xia, F., Jiang, C.L., Zhao, Y.P., He, L., “Nanoencapsulation of lutein with hydroxypropylmethyl cellulose phthalate by supercritical antisolvent”, Chin. J. Chem. Eng., 17(4), 672-677(2009).
21 Brunner, G., “Supercritical fluids: technology and application to food processing”, J. Food Eng., 67, 21-33(2005).
22 Quan, C., Johan, C., Charlotta, T., “Carotenoids particle formation by supercritical fluid technologies”, Chin. J. Chem. Eng., 17(2), 344-349(2009).
23 Magnan, C., Badens, E., Commenges, N., “Soy lecithin micronization by precipitation with a compressed fluid antisolvent influence of process parameters”, J. Supercrit. Fluids, 19, 69-77(2000).
24 Badens, E., Magnan, C., Charbit, G., “Microparticles of soy lecithin formed by supercritical processes”, Biotech. Bioeng., 72, 194-204(2001).
25 Kunastitchai, S., Pichert, L., Sarisuta, N., “Application of aerosol solvent extraction system(ASES) process for preparation of liposomes in a dry and reconstitutable form”, Int. J. Pharm., 316, 93-101(2006).
26 Yalkowsky, R.Y., Halothane, S.H., “A novel solvent for the preparation of liposomes containing 2-4′-amino-3′-methylphenyl benzothiazole(AMPB), an anticancer drug: a technical note”, AAPS Pharm. Sci. Tech., 4(2), 1-5(2003).
27 Chattopadhyay, P., Shekunov, B.Y., Yim, D., “Production of solid lipid nanoparticles suspensions using supercritical fluid extraction of emulsions(SFEE) for pulmonary delivery using the AERx system”, Adv. Drug Delivery Rev., 59, 444-453(2007).
28 Polozova, A., Li, X., Shangguana, T., Meers, P., Schuette, D.R., Ando, N., Gruner, S.M., Perkins, W.R., Formation of homogeneous unilamellar liposomes from an interdigitated matrix”, Biochim. Biophys. Acta, 1668, 117-125(2005).

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Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics

Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics

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