SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (3): 332-340.DOI: 10.1016/S1004-9541(13)60465-6

• 能源、资源与环境技术 • 上一篇    

Identification and Metabolic Mechanism of Non-fermentative Short-cut Denitrifying Phosphorus-removing Bacteria

刘晖1,2,3, 孙彦富2, 贾晓珊1, 李军3, 周康群2, 屈向东1,2, 陶雪琴2, 陈瑜3   

  1. 1 School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China ;
    2 Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China ;
    3 Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
  • 收稿日期:2011-12-05 修回日期:2012-04-05 出版日期:2013-03-28 发布日期:2013-04-01
  • 通讯作者: JIA Xiaoshan
  • 基金资助:

    Supported by the National Natural Science Foundation of China (51078008), the Natural Science Foundation of Guangdong Province (06022869, 07003251), and the National Key Scientific and Technological Project Water Pollution Control and Treatment (2008ZX07211-003, 2009ZX07314-009-003).

Identification and Metabolic Mechanism of Non-fermentative Short-cut Denitrifying Phosphorus-removing Bacteria

LIU Hui1,2,3, SUN Yanfu2, JIA Xiaoshan1, LI Jun3, ZHOU Kangqun2, QU Xiangdong1,2, TAO Xueqin2, CHEN Yu3   

  1. 1 School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China ;
    2 Department of Environment Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China ;
    3 Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
  • Received:2011-12-05 Revised:2012-04-05 Online:2013-03-28 Published:2013-04-01
  • Supported by:

    Supported by the National Natural Science Foundation of China (51078008), the Natural Science Foundation of Guangdong Province (06022869, 07003251), and the National Key Scientific and Technological Project Water Pollution Control and Treatment (2008ZX07211-003, 2009ZX07314-009-003).

摘要: To investigate the characteristics and metabolic mechanism of short-cut denitrifying phosphorus-removing bacteria (SDPB) that are capable of enhanced biological phosphorus removal (EBPR) using nitrite as an electron acceptor, an aerobic/anoxic sequencing batch reactor was operated under three phases. An SDPB-strain YC was screened after the sludge enrichment and was identified by morphological, physiological, biochemical properties and 16S rDNA gene sequence analysis. Denitrifying phosphorus-removing experiments were conducted to study anaerobic and anoxic metabolic mechanisms by analyzing the changes of chemical oxygen demand (COD), phosphate, nitrite, poly-β-hydroxybutyrate (PHB), and glycogen. The results show that strain YC is a non-fermentative SDPB similar to Paracoccus denitrificans. As a kind of non-fermentative bacteria, the energy of strain YC was mainly generated from phosphorus release (96.2%) under anaerobic conditions with 0.32 mg P per mg synthesized PHB. Under anoxic conditions, strain YC accumulated 0.45 mg P per mg degraded PHB, which produced most of energy for phosphate accumulation (91.3%) and a little for glycogen synthesis (8.7%). This metabolic mechanism of strain YC is different from that of traditional phosphorus-accumulating organisms (PAOs). It is also found that PHB, a kind of intracellular polymer, plays a very important role in denitrifying and accumulating phosphorus by supplying sufficient energy for phosphorous accumulation and carbon sources for denitrification. Therefore, monitoring ΔP/ΔPHB and? ΔNO2--N/ΔPHB is more necessary than monitoring ΔP/ΔCOD,?ΔNO2--N/ΔCOD, or ΔNO2--N.

关键词: short-cut denitrifying phosphorus removing bacteria, Paracoccus denitrificans, non-fermentative bacteria, metabolic mechanism, poly-&, beta, -hydroxybutyrate

Abstract: To investigate the characteristics and metabolic mechanism of short-cut denitrifying phosphorus-removing bacteria (SDPB) that are capable of enhanced biological phosphorus removal (EBPR) using nitrite as an electron acceptor, an aerobic/anoxic sequencing batch reactor was operated under three phases. An SDPB-strain YC was screened after the sludge enrichment and was identified by morphological, physiological, biochemical properties and 16S rDNA gene sequence analysis. Denitrifying phosphorus-removing experiments were conducted to study anaerobic and anoxic metabolic mechanisms by analyzing the changes of chemical oxygen demand (COD), phosphate, nitrite, poly-β-hydroxybutyrate (PHB), and glycogen. The results show that strain YC is a non-fermentative SDPB similar to Paracoccus denitrificans. As a kind of non-fermentative bacteria, the energy of strain YC was mainly generated from phosphorus release (96.2%) under anaerobic conditions with 0.32 mg P per mg synthesized PHB. Under anoxic conditions, strain YC accumulated 0.45 mg P per mg degraded PHB, which produced most of energy for phosphate accumulation (91.3%) and a little for glycogen synthesis (8.7%). This metabolic mechanism of strain YC is different from that of traditional phosphorus-accumulating organisms (PAOs). It is also found that PHB, a kind of intracellular polymer, plays a very important role in denitrifying and accumulating phosphorus by supplying sufficient energy for phosphorous accumulation and carbon sources for denitrification. Therefore, monitoring ΔP/ΔPHB and? ΔNO2--N/ΔPHB is more necessary than monitoring ΔP/ΔCOD,?ΔNO2--N/ΔCOD, or ΔNO2--N.

Key words: short-cut denitrifying phosphorus removing bacteria, Paracoccus denitrificans, non-fermentative bacteria, metabolic mechanism, poly-&, beta, -hydroxybutyrate