SCI和EI收录∣中国化工学会会刊

›› 2015, Vol. 23 ›› Issue (2): 451-455.DOI: 10.1016/j.cjche.2014.06.036

• 研究简报 • 上一篇    下一篇

Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

Sheng Hu1, Yingying Ma2, Hailong Jiang3, Dingjun Feng3, Wei Yu3, Dongmei Dai1, Lehe Mei1,2   

  1. 1 College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China;
    2 Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China;
    3 Ningbo Liwah Pharmaceutical Co., Ltd., Ningbo 315174, China
  • 收稿日期:2013-10-03 修回日期:2014-06-16 出版日期:2015-02-28 发布日期:2015-03-01
  • 通讯作者: Lehe Mei
  • 基金资助:
    Supported by the National Natural Science Foundation of China (21176220, 31240054), Key Technology Research and Development Project of Ningbo (2011C11023), and Zhejiang Provincial Natural Science Foundation of China (Z13B060008).

Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

Sheng Hu1, Yingying Ma2, Hailong Jiang3, Dingjun Feng3, Wei Yu3, Dongmei Dai1, Lehe Mei1,2   

  1. 1 College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China;
    2 Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China;
    3 Ningbo Liwah Pharmaceutical Co., Ltd., Ningbo 315174, China
  • Received:2013-10-03 Revised:2014-06-16 Online:2015-02-28 Published:2015-03-01
  • Supported by:
    Supported by the National Natural Science Foundation of China (21176220, 31240054), Key Technology Research and Development Project of Ningbo (2011C11023), and Zhejiang Provincial Natural Science Foundation of China (Z13B060008).

摘要: In order to facilitate the preparation of paeoniflorin (PF) and albiflorin (AF), two chief bioactive constituents in Paeonia lactiflora Pall (PL), induction and culture of callus from PL were studied. With a modified woody plant medium supplemented with 0.5 mg·L-1 6-benzylaminopurine, 1.0 mg·L-1 naphthylacetic acid, 0.1 mg·L-1 thidiazuron and 30 g·L-1 sucrose, callus was induced from four kinds of explants: leaf, stems, petiole, and root. The potency to formcallus varies between different explants and leaf explants exhibits the highest capacity (100%). On the other hand, root-derived callus (R-callus) produces the highest level of total amount of PF and AF, 31.8 mg·g-1 dry mass, which is higher than the corresponding level in the root of field cultivated PL. Furthermore, the timeneeded is only 40 days, remarkably shorter than the cultivation timeof PL, about 4-5years. Higher accumulation levels of PF and AF with shorter production time indicate that callus culture of PL is a promising powerful tool for production of PF and AF in the future.

关键词: Albiflorin, Paeoniflorin, Callus, Plant cell cultures, Paeonia lactiflora Pall

Abstract: In order to facilitate the preparation of paeoniflorin (PF) and albiflorin (AF), two chief bioactive constituents in Paeonia lactiflora Pall (PL), induction and culture of callus from PL were studied. With a modified woody plant medium supplemented with 0.5 mg·L-1 6-benzylaminopurine, 1.0 mg·L-1 naphthylacetic acid, 0.1 mg·L-1 thidiazuron and 30 g·L-1 sucrose, callus was induced from four kinds of explants: leaf, stems, petiole, and root. The potency to formcallus varies between different explants and leaf explants exhibits the highest capacity (100%). On the other hand, root-derived callus (R-callus) produces the highest level of total amount of PF and AF, 31.8 mg·g-1 dry mass, which is higher than the corresponding level in the root of field cultivated PL. Furthermore, the timeneeded is only 40 days, remarkably shorter than the cultivation timeof PL, about 4-5years. Higher accumulation levels of PF and AF with shorter production time indicate that callus culture of PL is a promising powerful tool for production of PF and AF in the future.

Key words: Albiflorin, Paeoniflorin, Callus, Plant cell cultures, Paeonia lactiflora Pall