Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine

doi:10.1016/j.cjche.2014.11.001

Chinese Journal of Chemical Engineering ›› 2015, Vol. 23 ›› Issue (1): 227-233.DOI: 10.1016/j.cjche.2014.11.001

Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine

Huixing Li^1,2, Ruijing Zhang¹, Lei Tang¹, Jianhua Zhang¹, Zhonggui Mao¹

1 The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;
2 School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, China

收稿日期:2013-07-22 修回日期:2013-11-01 出版日期:2015-01-28 发布日期:2015-01-24
通讯作者: Lei Tang, Zhonggui Mao
基金资助:
Supported by the Science & Technology Programof Jiangsu Province (BE2011623) and the Scientific Research Project of Provincial Environmental Protection Bureau of Jiangsu Province (2012047).

Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine

Huixing Li^1,2, Ruijing Zhang¹, Lei Tang¹, Jianhua Zhang¹, Zhonggui Mao¹

1 The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;
2 School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, China

Received:2013-07-22 Revised:2013-11-01 Online:2015-01-28 Published:2015-01-24
Contact: Lei Tang, Zhonggui Mao
Supported by:
Supported by the Science & Technology Programof Jiangsu Province (BE2011623) and the Scientific Research Project of Provincial Environmental Protection Bureau of Jiangsu Province (2012047).

摘要/Abstract

摘要： Bioconversion of lignocellulosic wastes to higher value products through fungal fermentation has economic and ecological benefits. In this study, to develop an effective strategy for production of manganese peroxidase (MnP) fromcassava residue by Phanerochaete chrysosporiumin solid state fermentation, the stimulators of MnP production were screened and their concentrations were optimized by one-at-a-time experiment and Box-Behnken design. The maximum MnP activity of 186.38 nkat·g^-1 dry mass of the sample was achieved after 6 days of fermentation with the supplement of 79.5mmol·L^-1·kg^-1 acetic acid, 3.21 ml·kg^-1 soybean oil, and 28.5 g·kg^-1 alkaline lignin, indicating that cassava residue is a promising substrate for MnP production in solid state fermentation. Meanwhile, in vitro decolorization of indigo carmine by the crude MnP was also carried out, attaining the ratio of 90.18% after 6 h of incubation. An oxidative mechanism of indigo carmine decolorization by MnP was proposed based on the analysis of intermediate metabolites with ultra-high performance liquid chromatography and gas chromatography tandemmass spectrometry. Using the crude MnP produced fromcassava residue for indigo carmine decolorization gives an effective approach to treat dyeing effluents.

关键词: Cassava residue, Manganese peroxidase, Phanerochaete chrysosporium, Solid state fermentation, Indigo carmine

Abstract: Bioconversion of lignocellulosic wastes to higher value products through fungal fermentation has economic and ecological benefits. In this study, to develop an effective strategy for production of manganese peroxidase (MnP) fromcassava residue by Phanerochaete chrysosporiumin solid state fermentation, the stimulators of MnP production were screened and their concentrations were optimized by one-at-a-time experiment and Box-Behnken design. The maximum MnP activity of 186.38 nkat·g^-1 dry mass of the sample was achieved after 6 days of fermentation with the supplement of 79.5mmol·L^-1·kg^-1 acetic acid, 3.21 ml·kg^-1 soybean oil, and 28.5 g·kg^-1 alkaline lignin, indicating that cassava residue is a promising substrate for MnP production in solid state fermentation. Meanwhile, in vitro decolorization of indigo carmine by the crude MnP was also carried out, attaining the ratio of 90.18% after 6 h of incubation. An oxidative mechanism of indigo carmine decolorization by MnP was proposed based on the analysis of intermediate metabolites with ultra-high performance liquid chromatography and gas chromatography tandemmass spectrometry. Using the crude MnP produced fromcassava residue for indigo carmine decolorization gives an effective approach to treat dyeing effluents.

Key words: Cassava residue, Manganese peroxidase, Phanerochaete chrysosporium, Solid state fermentation, Indigo carmine

Huixing Li, Ruijing Zhang, Lei Tang, Jianhua Zhang, Zhonggui Mao. Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine[J]. Chinese Journal of Chemical Engineering, 2015, 23(1): 227-233.

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Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine

Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine

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