Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption

doi:10.1016/j.cjche.2015.02.002

Chinese Journal of Chemical Engineering ›› 2015, Vol. 23 ›› Issue (5): 822-826.DOI: 10.1016/j.cjche.2015.02.002

Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption

Ya Li^1,2, Jicheng Hu¹, Pingfang Han¹

1 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, China;
2 Department of Environment and Resource, Nantong Science and Technology College, Nantong 226007, China

收稿日期:2013-10-08 修回日期:2014-02-24 出版日期:2015-05-28 发布日期:2015-06-26
通讯作者: Pingfang Han
基金资助:
Supported by the National Basic Research Program of China (2009CB724700) and the Foundation of Jiangsu Province of China for College Postgraduate Students in Innovation Engineering (CXZZ12_0440).

Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption

Ya Li^1,2, Jicheng Hu¹, Pingfang Han¹

1 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, China;
2 Department of Environment and Resource, Nantong Science and Technology College, Nantong 226007, China

Received:2013-10-08 Revised:2014-02-24 Online:2015-05-28 Published:2015-06-26
Contact: Pingfang Han
Supported by:
Supported by the National Basic Research Program of China (2009CB724700) and the Foundation of Jiangsu Province of China for College Postgraduate Students in Innovation Engineering (CXZZ12_0440).

摘要/Abstract

摘要： Magnetically modified palygorskite composites were synthesized with γ-Fe₂O₃ dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99-125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of 41.5mg·g^-1 support and enzyme activity of 2631.6 U·(g support)^-1. The immobilized lipase exhibit better thermal and broader pH stability and excellent reusability compared with free lipase.

关键词: Magnetically modified palygorskite, Immobilized lipase, Adsorption, Stability, Reusability

Abstract: Magnetically modified palygorskite composites were synthesized with γ-Fe₂O₃ dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99-125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of 41.5mg·g^-1 support and enzyme activity of 2631.6 U·(g support)^-1. The immobilized lipase exhibit better thermal and broader pH stability and excellent reusability compared with free lipase.

Key words: Magnetically modified palygorskite, Immobilized lipase, Adsorption, Stability, Reusability

Ya Li, Jicheng Hu, Pingfang Han. Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption[J]. Chinese Journal of Chemical Engineering, 2015, 23(5): 822-826.

Ya Li, Jicheng Hu, Pingfang Han. Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption[J]. Chin.J.Chem.Eng., 2015, 23(5): 822-826.

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Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption

Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption

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