SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2015, Vol. 23 ›› Issue (5): 822-826.DOI: 10.1016/j.cjche.2015.02.002

• 生物技术与生物工程 • 上一篇    下一篇

Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption

Ya Li1,2, Jicheng Hu1, Pingfang Han1   

  1. 1 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, China;
    2 Department of Environment and Resource, Nantong Science and Technology College, Nantong 226007, China
  • 收稿日期:2013-10-08 修回日期:2014-02-24 出版日期:2015-05-28 发布日期:2015-06-26
  • 通讯作者: Pingfang Han
  • 基金资助:

    Supported by the National Basic Research Program of China (2009CB724700) and the Foundation of Jiangsu Province of China for College Postgraduate Students in Innovation Engineering (CXZZ12_0440).

Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption

Ya Li1,2, Jicheng Hu1, Pingfang Han1   

  1. 1 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, China;
    2 Department of Environment and Resource, Nantong Science and Technology College, Nantong 226007, China
  • Received:2013-10-08 Revised:2014-02-24 Online:2015-05-28 Published:2015-06-26
  • Contact: Pingfang Han
  • Supported by:

    Supported by the National Basic Research Program of China (2009CB724700) and the Foundation of Jiangsu Province of China for College Postgraduate Students in Innovation Engineering (CXZZ12_0440).

摘要: Magnetically modified palygorskite composites were synthesized with γ-Fe2O3 dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99-125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of 41.5mg·g-1 support and enzyme activity of 2631.6 U·(g support)-1. The immobilized lipase exhibit better thermal and broader pH stability and excellent reusability compared with free lipase.

关键词: Magnetically modified palygorskite, Immobilized lipase, Adsorption, Stability, Reusability

Abstract: Magnetically modified palygorskite composites were synthesized with γ-Fe2O3 dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99-125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of 41.5mg·g-1 support and enzyme activity of 2631.6 U·(g support)-1. The immobilized lipase exhibit better thermal and broader pH stability and excellent reusability compared with free lipase.

Key words: Magnetically modified palygorskite, Immobilized lipase, Adsorption, Stability, Reusability