SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2021, Vol. 29 ›› Issue (2): 54-61.DOI: 10.1016/j.cjche.2020.09.001

• Synthetic Biotechnology and Metabolic Engineering • 上一篇    下一篇

Recent advances in microbial production of phenolic compounds

Xinxiao Sun, Xianglai Li, Xiaolin Shen, Jia Wang, Qipeng Yuan   

  1. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China
  • 收稿日期:2020-08-02 修回日期:2020-08-30 出版日期:2021-02-28 发布日期:2021-05-15
  • 通讯作者: Qipeng Yuan
  • 基金资助:
    This work was supported by National Key Research and Development Program of China (2018YFA0901800 and 2018YFA0901400) and National Natural Science Foundation of China (21978015, 21636001, and 21776008).

Recent advances in microbial production of phenolic compounds

Xinxiao Sun, Xianglai Li, Xiaolin Shen, Jia Wang, Qipeng Yuan   

  1. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China
  • Received:2020-08-02 Revised:2020-08-30 Online:2021-02-28 Published:2021-05-15
  • Contact: Qipeng Yuan
  • Supported by:
    This work was supported by National Key Research and Development Program of China (2018YFA0901800 and 2018YFA0901400) and National Natural Science Foundation of China (21978015, 21636001, and 21776008).

摘要: Phenolic compounds (PCs) are a group of compounds with various applications in nutraceutical, pharmaceutical and cosmetic industries. Their supply by plant extraction and chemical synthesis is often limited by low yield and high cost. Microbial production represents as a promising alternative for efficient and sustainable production of PCs. In this review, we summarize recent advances in this field, which include enzyme mining and engineering to construct artificial pathways, balance of enzyme expression to improve pathway efficiency, coculture engineering to alleviate metabolic burden and side-reactions, and the use of genetic circuits for dynamic regulation and high throughput screening. Finally, current challenges and future perspectives for efficient production of PCs are also discussed.

关键词: Phenolic compounds, Pathway design, Protein engineering, Coculture engineering, Modular pathway optimization, Dynamic regulation

Abstract: Phenolic compounds (PCs) are a group of compounds with various applications in nutraceutical, pharmaceutical and cosmetic industries. Their supply by plant extraction and chemical synthesis is often limited by low yield and high cost. Microbial production represents as a promising alternative for efficient and sustainable production of PCs. In this review, we summarize recent advances in this field, which include enzyme mining and engineering to construct artificial pathways, balance of enzyme expression to improve pathway efficiency, coculture engineering to alleviate metabolic burden and side-reactions, and the use of genetic circuits for dynamic regulation and high throughput screening. Finally, current challenges and future perspectives for efficient production of PCs are also discussed.

Key words: Phenolic compounds, Pathway design, Protein engineering, Coculture engineering, Modular pathway optimization, Dynamic regulation