重组大肠杆菌分批-补料高密度发酵生产类人胶原蛋白的动力学

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重组大肠杆菌分批-补料高密度发酵生产类人胶原蛋白的动力学

花秀夫^a; 范代娣^a,d; 骆艳娥^a; 张兮^d; 施惠娟^b; 米钰^a; 马晓轩^a; 尚龙安^a,c; 赵桂仿^d

^a Shaanxi Source and Application Chemical Engineering Center, Department of Chemical Engineering, Northwest University, Xi’an 710069, China
^b Department of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
c College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China
d State Education Ministry Key Laboratory of Resource Biotechnology in Western China, Department of Biology, Northwest University, Xi’an 710069, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-04-28 发布日期:2006-04-28
通讯作者: 范代娣

Kinetics of High Cell Density Fed-batch Culture of Recombinant
Escherichia coli Producing Human-like Collagen

HUA Xiufu^a; FAN Daidi^a,d; LUO Yan'e^a; ZHANG Xi^d; SHI Huijuan^b; MI Yu^a; MA Xiaoxuan^a; SHANG Long'an^a,c; ZHAO Guifang^d

^a Shaanxi Source and Application Chemical Engineering Center, Department of Chemical Engineering, Northwest University, Xi’an 710069, China
^b Department of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
c College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China
d State Education Ministry Key Laboratory of Resource Biotechnology in Western China, Department of Biology, Northwest University, Xi’an 710069, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-04-28 Published:2006-04-28
Contact: FAN Daidi

摘要/Abstract

摘要： The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded without consideration of cell endogeneous metabolism. The cell lag time, the maximum specific growth rate and YX/S were determined as 1.75h, 0.65h－1 and 0.51g•g－1, respectively. In the fed-batch culture, different specific growth rates were set at (0.15, 0.2, 0.25h－1) by the method of pseudo-exponential feeding, and the expressions for the specific rate of substrate consumption, the growth kinetics and the product formation kinetics of each phase were obtained. The result shows that the concentrations of cell and product can reach 77.5g•L－1 and 10.2g•L－1 respectively. The model predictions are in good agreement with the experimental data.

关键词: human-like collagen;kinetics;high density fermentation;Escherichia coli

Abstract: The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded without consideration of cell endogeneous metabolism. The cell lag time, the maximum specific growth rate and YX/S were determined as 1.75h, 0.65h－1 and 0.51g•g－1, respectively. In the fed-batch culture, different specific growth rates were set at (0.15, 0.2, 0.25h－1) by the method of pseudo-exponential feeding, and the expressions for the specific rate of substrate consumption, the growth kinetics and the product formation kinetics of each phase were obtained. The result shows that the concentrations of cell and product can reach 77.5g•L－1 and 10.2g•L－1 respectively. The model predictions are in good agreement with the experimental data.

Key words: human-like collagen, kinetics, high density fermentation, Escherichia coli

花秀夫, 范代娣, 骆艳娥, 张兮, 施惠娟, 米钰, 马晓轩, 尚龙安, 赵桂仿. 重组大肠杆菌分批-补料高密度发酵生产类人胶原蛋白的动力学
[J]. , DOI: .

HUA Xiufu, FAN Daidi a,d , LUO Yan'e, ZHANG Xi, SHI Huijuan, MI Yu, MA Xiaoxuan, SHANG Long'an, ZHAO Guifang. Kinetics of High Cell Density Fed-batch Culture of Recombinant
Escherichia coli Producing Human-like Collagen
[J]. , DOI: .

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