Improve Ethanol Yield Through Minimizing Glycerol Yield in Ethanol Fermentation of Saccharomyces cerevisiae

›› 2008, Vol. 16 ›› Issue (4): 620-625.

Improve Ethanol Yield Through Minimizing Glycerol Yield in Ethanol Fermentation of Saccharomyces cerevisiae

张爱利, 陈洵

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

收稿日期:2007-08-13 修回日期:2008-03-26 出版日期:2008-08-28 发布日期:2008-08-28
通讯作者: CHEN Xun, E-mail: chenxun@tju.edu.cn
基金资助:
the National High Technology Research and Development Program of China(2002AA647040)

Improve Ethanol Yield Through Minimizing Glycerol Yield in Ethanol Fermentation of Saccharomyces cerevisiae

ZHANG Aili, CHEN Xun

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

Received:2007-08-13 Revised:2008-03-26 Online:2008-08-28 Published:2008-08-28
Supported by:
the National High Technology Research and Development Program of China(2002AA647040)

摘要/Abstract

摘要： In ethanol fermentation of Saccharomyces cerevisiae (S. cerevisiae), glycerol is one of the main by-products. The purpose of this investigation was to increase ethanol yield through minimizing glycerol yield by using mutants in which FPS1 encoding a channel protein that mediates glycerol export and GPD2 encoding one of glycerol-3-phosphate dehydrogenase were knocked-out using one-step gene replacement. GLT1 and GLN1 that encode glutamate synthase and glutamine synthetase, respectively, were overexpressed using two-step gene replacement in fps1Δgpd2Δ mutant. The fermentation properties of ZAL69(fps1Δ::LEU2 gpd2Δ::URA3) and ZAL808 (fps1Δ::LEU2 gpd2Δ::URA3 _PPGK1-GLT1 _PPGK1-GLN1) under microaerobic conditions were investigated and compared with those of wild type(DC124). Consumption of glucose, yield of ethanol, yield of glycerol, acetic acid, and pyruvic acid were monitored. Compared with wild type, the ethanol yield of ZAL69 and ZAL808 were improved by 13.17% and 6.66%, respectively, whereas glycerol yield decreased by 37.4% and 41.7%. Meanwhile, acetic acid yield and pyruvic acid yield decreased dramatically compared to wild type. Our results indicate that FPS1 and GPD2 deletion of S. cerevisiae resulted in reduced glycerol yield and increased ethanol yield, but simultaneous over-expression of GLT1 and GLN1 in fps1Δgpd2Δ mutant did not have a higher ethanol yield than fps1Δgpd2Δ mutant.

关键词: Saccharomyces cerevisiae, ethanol yield, glycerol yield, gene knock-out, gene over-express, FPS1, GPD2, GLN1, GLT1

Abstract: In ethanol fermentation of Saccharomyces cerevisiae (S. cerevisiae), glycerol is one of the main by-products. The purpose of this investigation was to increase ethanol yield through minimizing glycerol yield by using mutants in which FPS1 encoding a channel protein that mediates glycerol export and GPD2 encoding one of glycerol-3-phosphate dehydrogenase were knocked-out using one-step gene replacement. GLT1 and GLN1 that encode glutamate synthase and glutamine synthetase, respectively, were overexpressed using two-step gene replacement in fps1Δgpd2Δ mutant. The fermentation properties of ZAL69(fps1Δ::LEU2 gpd2Δ::URA3) and ZAL808 (fps1Δ::LEU2 gpd2Δ::URA3 _PPGK1-GLT1 _PPGK1-GLN1) under microaerobic conditions were investigated and compared with those of wild type(DC124). Consumption of glucose, yield of ethanol, yield of glycerol, acetic acid, and pyruvic acid were monitored. Compared with wild type, the ethanol yield of ZAL69 and ZAL808 were improved by 13.17% and 6.66%, respectively, whereas glycerol yield decreased by 37.4% and 41.7%. Meanwhile, acetic acid yield and pyruvic acid yield decreased dramatically compared to wild type. Our results indicate that FPS1 and GPD2 deletion of S. cerevisiae resulted in reduced glycerol yield and increased ethanol yield, but simultaneous over-expression of GLT1 and GLN1 in fps1Δgpd2Δ mutant did not have a higher ethanol yield than fps1Δgpd2Δ mutant.

Key words: Saccharomyces cerevisiae, ethanol yield, glycerol yield, gene knock-out, gene over-express, FPS1, GPD2, GLN1, GLT1

张爱利, 陈洵. Improve Ethanol Yield Through Minimizing Glycerol Yield in Ethanol Fermentation of Saccharomyces cerevisiae[J]. , 2008, 16(4): 620-625.

ZHANG Aili, CHEN Xun. Improve Ethanol Yield Through Minimizing Glycerol Yield in Ethanol Fermentation of Saccharomyces cerevisiae[J]. , 2008, 16(4): 620-625.

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Improve Ethanol Yield Through Minimizing Glycerol Yield in Ethanol Fermentation of Saccharomyces cerevisiae

Improve Ethanol Yield Through Minimizing Glycerol Yield in Ethanol Fermentation of Saccharomyces cerevisiae

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