Two-step Enzymatic Synthesis of Guanine Arabinoside

›› 2008, Vol. 16 ›› Issue (6): 934-937.

Two-step Enzymatic Synthesis of Guanine Arabinoside

魏晓琨^1,2,3, 丁庆豹¹, 欧伶¹, 张鹭³, 王昌禄²

1. State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;
2. Tianjin University of Science and Technology, Tianjin 300222, China;
3. Shanghai Weiping Bioscience and Biotechnology Co., Ltd., Shanghai 200237, China

收稿日期:2008-02-18 修回日期:2008-08-25 出版日期:2008-12-28 发布日期:2008-12-28
通讯作者: DING Qingba,E-mail:bioxianleid@163.com
基金资助:
Supported by the Innovation Fund for Technology Based Firms from Ministry of Science and Technology of China (07C26213101283)

Two-step Enzymatic Synthesis of Guanine Arabinoside

WEI Xiaokun^1,2,3, DING Qingbao¹, OU Lin¹, ZHANG Lu³, WANG Changlu²

1. State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;
2. Tianjin University of Science and Technology, Tianjin 300222, China;
3. Shanghai Weiping Bioscience and Biotechnology Co., Ltd., Shanghai 200237, China

Received:2008-02-18 Revised:2008-08-25 Online:2008-12-28 Published:2008-12-28
Supported by:
Supported by the Innovation Fund for Technology Based Firms from Ministry of Science and Technology of China (07C26213101283)

摘要/Abstract

摘要： The chemical synthesis of Guanine arabinoside(ara-G)is extremely complex,time-consuming,and seriously polluted.A two-step enzymatic synthesis process was developed to acquire ara-G easily.2,6-Diaminopurine arabinoside(ara-DA)was first synthesized with purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase produced by Enterobacter aerogenes DGW-07.The conversion yield of ara-DA could reach above 90% when the reaction liquid contained 30 mmol·L^-1 uracil arabinoside as arabinose donor,10 mmol·L^-1 2,6-diaminopurine as arabinose acceptor in pH7.0 20 mmol·L^-1 phosphate buffer,and reacted at 60℃ for 48h.Then,ara-DA was effectively transformed into ara-G with adenylate deaminase produced by Aspergillus oryzae DAW-01.The total process had no complex separation and purification.

关键词: guanine arabinoside, 2,6-diaminopurine arabinoside, purine nucleoside phosphorylase, pyrimidine nucleoside phosphorylase, adenylate deaminase, Enterobacter aerogenes, Aspergillus oryzae

Abstract: The chemical synthesis of Guanine arabinoside(ara-G)is extremely complex,time-consuming,and seriously polluted.A two-step enzymatic synthesis process was developed to acquire ara-G easily.2,6-Diaminopurine arabinoside(ara-DA)was first synthesized with purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase produced by Enterobacter aerogenes DGW-07.The conversion yield of ara-DA could reach above 90% when the reaction liquid contained 30 mmol·L^-1 uracil arabinoside as arabinose donor,10 mmol·L^-1 2,6-diaminopurine as arabinose acceptor in pH7.0 20 mmol·L^-1 phosphate buffer,and reacted at 60℃ for 48h.Then,ara-DA was effectively transformed into ara-G with adenylate deaminase produced by Aspergillus oryzae DAW-01.The total process had no complex separation and purification.

Key words: guanine arabinoside, 2,6-diaminopurine arabinoside, purine nucleoside phosphorylase, pyrimidine nucleoside phosphorylase, adenylate deaminase, Enterobacter aerogenes, Aspergillus oryzae

魏晓琨, 丁庆豹, 欧伶, 张鹭, 王昌禄. Two-step Enzymatic Synthesis of Guanine Arabinoside[J]. , 2008, 16(6): 934-937.

WEI Xiaokun, DING Qingbao, OU Lin, ZHANG Lu, WANG Changlu. Two-step Enzymatic Synthesis of Guanine Arabinoside[J]. , 2008, 16(6): 934-937.

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Two-step Enzymatic Synthesis of Guanine Arabinoside

Two-step Enzymatic Synthesis of Guanine Arabinoside

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