Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production

›› 2010, Vol. 18 ›› Issue (4): 681-686.

Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production

陈火晴^1,2, 马晓轩^1,2, 范代娣^1,2, 骆艳娥^1,2, 高鹏飞^1,2, 杨婵媛^1,2

1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
2. Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical and Engineering, Northwest University, Xi'an 710069, China

收稿日期:2009-11-20 修回日期:2010-05-20 出版日期:2010-08-28 发布日期:2010-08-28
通讯作者: FAN Daidi, E-mail:fandaidi@nwu.edu.cn
基金资助:
Supported by the National High Technology Research and Development Program of China(2006AA02Z246,2007AA03Z456);the National Natural Science Foundation of China(20776119);the Specialized Research Fund for the Doctoral Program of Higher Education of China(20096101120023);Shaanxi Provincial Natural Science Foundation(SJ08B03);Shaanxi Key Subject Program,China

Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production

CHEN Huoqing^1,2, MA Xiaoxuan^1,2, FAN Daidi^1,2, LUO Yan'e^1,2, GAO Pengfei^1,2, YANG Chanyuan^1,2

1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
2. Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical and Engineering, Northwest University, Xi'an 710069, China

Received:2009-11-20 Revised:2010-05-20 Online:2010-08-28 Published:2010-08-28

摘要/Abstract

摘要： The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L^-1,while it had negative effects when its concentration was higher than 500 mg·L^-1.The hydrogen production was the highest 1.29 mol·mol^-1(H₂/glucose) when 300 mg·L^-1 L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP),which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range -300 mV to -150 mV when the L-cysteine concentration was higher than 500 mg·L^-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L^-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.

关键词: anaerobic fermentation, biohydrogen, L-cysteine, ORP

Abstract: The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L^-1,while it had negative effects when its concentration was higher than 500 mg·L^-1.The hydrogen production was the highest 1.29 mol·mol^-1(H₂/glucose) when 300 mg·L^-1 L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP),which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range -300 mV to -150 mV when the L-cysteine concentration was higher than 500 mg·L^-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L^-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.

Key words: anaerobic fermentation, biohydrogen, L-cysteine, ORP

陈火晴, 马晓轩, 范代娣, 骆艳娥, 高鹏飞, 杨婵媛. Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production[J]. , 2010, 18(4): 681-686.

CHEN Huoqing, MA Xiaoxuan, FAN Daidi, LUO Yan'e, GAO Pengfei, YANG Chanyuan. Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production[J]. , 2010, 18(4): 681-686.

参考文献

1 Hao,X. L.,Zhou,M. H.,Yu,H. Q.,Shen,Q.Q.,Lei,L. C.,“Effect of sodium ion concentration on hydrogen production from sucrose by anaerobic hydrogen-producing granular sludge”,Chin.J.Chem. Eng.,14(4),511-517(2006).
2 Demirbas,A.,“Progress and recent trends in biofuels”,Prog.Energy Combust.Sci.,33(1),1-18(2007).
3 Das,D.,“Advances in biohydrogen production processes:An ap-proach towards commercialization”,Int.J.Hydrogen Energy,34 (17),7349-7357(2009).
4 Wang,Y. B.,“The most promising energy in the 21century-hydrogen”, Energy Res.Inf.,19(2),63-68(2003).
5 Elam,C.C.,Gregoire Padro,C.E.,Sandrock,G.,Luzzi,A.,Lindblad, P.,Hagen,E.F.,“Realizing the hydrogen future:the International Energy Agency's efforts to advance hydrogen energy technologies”, Int. J.Hydrogen Energy,28(6),601-607(2003).
6 Wu,S. Y.,Huang,C. H.,Lin,C. N.,Chen,H. W.,Lee,A.S.,Chang, J.S.,“Fermentation hydrogen production and bacteria community structure in high-rate anaerobic bioreactors containing silicone-immobilized and self-flocculated sludge”,Biotechnol.Bioeng., 93,934-946(2006).
7 Dolores,E.,Marta,M.,Carlos,M.G.A.,“L-Cysteine,a thiol amino acid,increases the stimulating acute effect of ethanol on locomotion”,Alcohol,25,83-88(2001).
8 Niroshini,M.G.,Gregory,I.G.,Claus,J.,“Multiple roles of cysteine in biocatalysis”,Biochem.Biophys.Res.Commun.,300,1-4(2003).
9 Valdez-Vazquez,I.,Poggi-Varaldo,H.M.,“Hydrogen production by fermentative consortia”,Renew.Sust.Energ.Rew.,13(5),1000-1013 (2009).
10 Laurinavichene,T.V.,Chanal,A.,Wu,L.F.,Tsygankov,A.A.,“Effect of O₂,H₂ and redox potential on the activity and synthesis of hydrogenase 2 in Escherichia coli”,Res.Microbiol.,152(9),793-798 (2001).
11 Ren,Y.L.,Xing,X.H.,Zhang,C.,Gou,Z. X.,“A simplified method for assay of hydrogenase activities of H₂ evolution and uptake in Enterobacter aerogenes”,Biotechnol.Lett.,27,1029-1033(2005).
12 Karlsson,A.,Vallin,L.,Ejlertsson,J.,“Effects of temperature,hydraulic retention time and hydrogen extraction rate on hydrogen production from the fermentation of food industry residues and manure”,Int.J.Hydrogen Energy,33(3),953-962(2008).
13 Skonieczny,M.T.,Yargeau,V.,“Biohydrogen production from wastewater by Clostridium beijerinckii:Effect of pH and substrate concentration”,Int.J.Hydrogen Energy,34(8),3288-3294(2009).
14 Sompong,O.T.,Poonsuk,P.,Nils-kare,B.,“Evaluation of methods for preparing hydrogen-producing seed inocula under thermophilic condition by process performance and microbial community analysis”,Bioresour.Technol.,100,909-918(2009).
15 Li,J.Z.,Ren,N. Q.,Qing,Z.,Li,Y. F.,Bao,H. X.,Jiang,P.,“Startup of hydrogen producing fermentation process with anaerobic activated sludge and acclimatization of the dominant ethanol-type fermentation population”,High Technol.Lett.,14,90-94(2004).(in Chinese)
16 Li,J. Z.,Li,B. K.,Zhu,G. F.,Ren,N.Q.,Bo,L. X.,He,J. G.,“Hydrogen production from diluted molasses by anaerobic hydrogen producing bacteria in an anaerobic baffled reactor(ABR)”,Int.J.Hydrogen Energy,32(15),3274-3283(2007).
17 Liu,X. M.,Ren,N.Q.,Song,F.N.,Yang,C.P.,Wang,A.J.,“Recentadvances in fermentative biohydrogen production”,Progress in Natural Science,18(3),253-258(2008).
18 Ren,N.Q.,Wang,A. J.,Ma,F.,Acid-producing Fermentative Microbe Physiological Ecology,Science Press,Beijing(2005).(in Chinese)
19 Ding,J.,“The impacts of mental ions and L-cysteine on hydrogen production and control countermeasure”,Ph. D.Thesis,Harbin Institute of Technology,Harbin(2005).(in Chinese)
20 Lin,C. Y.,Wu,C. C.,Wu,J. H.,Chang,F. Y.,“Effect of cultivation temperature on fermentative hydrogen production from xylose by a mixed culture”,Biomass and Bioenergy,32(12),1109-1115(2008).
21 Chai,S. C.,Jerkins,A.A.,Banik,J.J.,Shalev,I.,Pinkham,J. L.,Uden, P.C.,Maroney,M.J.,“Heterologous expression,purification,and characterization of recombinant rat cysteine dioxygenase”,The Journal of Biological Chemistry,280,9865-9869(2005).
22 Chen,Y.K.,“Study on bacteria-inhibiting effects of cysteine and its SOD-like activity”,Journal of Shanxi University(Nat.Sci.Ed.),27, 65-67(2007).(in Chinese) 23 Chen,Y. K.,Xu,J.,Qu,Y.B.,Xi,G. P.,Zhang,M.,“Study on bacteria-inhabiting effect of amino acids”,Chinese Journal of Biochemical Pharmaceutics,(1),29-30(2001).(in Chinese)
24 Li,J. Z.,Ren,N.Q.,“The operational controlling strategy about the optimal fermentation type of acidogenic phase”,Chin.Environ.Sci., 18(5),398-402(1998).(in Chinese)
25 Ren,N.Q.,Wang,A.J.,Ma,F.,Physiological Ecology of Acidogens in Anaerobic Biotreatment Process,Science Press,Beijing(2005). (in Chinese)
26 Hallenbeck,P.C.,Ghosh,D.,“Advances in fermentative biohydrogen production:the way forward?”,Trends Biotechnol.,27(5),287-297 (2009).
27 Das,D.,Veziroglu,T.N.,“Advances in biological hydrogen production processes”,Int.J.Hydrogen Energy,33(21),6046-6057 (2008).
28 Lin,C.Y.,Lay,C.H.,“Carbon/nitrogen-ratio effect on fermentative hydrogen production by mixed microflora”,Int.J.Hydrogen Energy, 29(1),41-45(2004).
29 Wang,Y.,Ren,N.,Sun,Y.,Li,J. Z.,Sun,X.,“Analysis on the mechanism and capacity of two types of hydrogen production-ethanol fermentation and butyric acid fermentation”,Acta Energy Solaris Sinica,23(3),35-42(2002).
30 Kang,Y.H.,Yin,Y.X.,“Study on kinetic model of oxidation-reduction potential in acidogenic fermentation system”,J.Harbin.Univ.Commer.(Nat.Sci.Ed.),19(4),408-410(2003).(in Chinese)

[1]	Yingli Li, Zhishuncheng Li, Guangfei Qu, Rui Li, Shuaiyu Liang, Junhong Zhou, Wei Ji, Huiming Tang. Mechanism, behaviour and application of iron nitrate modified carbon nanotube composites for the adsorption of arsenic in aqueous solutions[J]. 中国化学工程学报, 2023, 60(8): 26-36.
[2]	Jing Huang, Honghui Cai, Qian Zhao, Yunpeng Zhou, Haibo Liu, Jing Wang. Dual-functional pyrene implemented mesoporous silicon material used for the detection and adsorption of metal ions[J]. 中国化学工程学报, 2023, 60(8): 108-117.
[3]	Lingli Chen, Yueting Shi, Sijun Xu, Junle Xiong, Fang Gao, Shengtao Zhang, Hongru Li. Enhanced adsorption of target branched compounds including antibiotic norfloxacin frameworks on mild steel surface for efficient protection: An experimental and molecular modelling study[J]. 中国化学工程学报, 2023, 60(8): 212-227.
[4]	Alexander Nti Kani, Evans Dovi, Aaron Albert Aryee, Runping Han, Zhaohui Li, Lingbo Qu. Mechanisms and reusability potentials of zirconium-polyaziridine-engineered tiger nut residue towards anionic pollutants[J]. 中国化学工程学报, 2023, 60(8): 275-292.
[5]	Yuan Liu, Hanting Xiong, Jingwen Chen, Shixia Chen, Zhenyu Zhou, Zheling Zeng, Shuguang Deng, Jun Wang. One-step ethylene separation from ternary C₂ hydrocarbon mixture with a robust zirconium metal-organic framework[J]. 中国化学工程学报, 2023, 59(7): 9-15.
[6]	Hui Jiang, Zijian Zhao, Ning Yu, Yi Qin, Zhengwei Luo, Wenhua Geng, Jianliang Zhu. Synthesis, characterization, and performance comparison of boron using adsorbents based on N-methyl-D-glucosamine[J]. 中国化学工程学报, 2023, 59(7): 16-31.
[7]	Sufei Wang, Mengjie Hao, Danyang Xiao, Tianmiao Zhang, Hua Li, Zhongshan Chen. Synthesis of porous carbon nanomaterials and their application in tetracycline removal from aqueous solutions[J]. 中国化学工程学报, 2023, 59(7): 200-209.
[8]	Runze Chen, Yuran Chen, Xuemin Liang, Yapeng Kong, Yangyang Fan, Quan Liu, Zhenyu Yang, Feiying Tang, Johnny Muya Chabu, Maru Dessie Walle, Liqiang Wang. Oxidative exfoliation of spent cathode carbon: A two-in-one strategy for its decontamination and high-valued application[J]. 中国化学工程学报, 2023, 59(7): 262-269.
[9]	Shanghong Ma, Haitao Zhang, Jianbo Qu, Xiuzhong Zhu, Qingfei Hu, Jianyong Wang, Peng Ye, Futao Sai, Shiwei Chen. Preparation of waterborne polyurethane/β-cyclodextrin composite nanosponge by ion condensation method and its application in removing of dyes from wastewater[J]. 中国化学工程学报, 2023, 58(6): 124-136.
[10]	Yutong Jiang, Yifeng Chen, Fuliu Yang, Jixue Fan, Jun Li, Zhuhong Yang, Xiaoyan Ji. Efficient SO₂ removal using aqueous ionic liquid at low partial pressure[J]. 中国化学工程学报, 2023, 58(6): 355-363.
[11]	Yueting Shi, Junhai Zhao, Lingli Chen, Hongru Li, Shengtao Zhang, Fang Gao. Double open mouse-like terpyridine parts based amphiphilic ionic molecules displaying strengthened chemical adsorption for anticorrosion of copper in sulfuric acid solution[J]. 中国化学工程学报, 2023, 57(5): 233-246.
[12]	Jian Wang, Yuanhui Shen, Donghui Zhang, Zhongli Tang, Wenbin Li. Integrated vacuum pressure swing adsorption and Rectisol process for CO₂ capture from underground coal gasification syngas[J]. 中国化学工程学报, 2023, 57(5): 265-279.
[13]	Yujia Cui, Zhiqiang Tan, Yanan Wang, Shuxian Shi, Xiaonong Chen. One-step crosslinking preparation of tannic acid particles for the adsorption and separation of cationic dyes[J]. 中国化学工程学报, 2023, 57(5): 309-318.
[14]	Shanshan Mao, Tao Shen, Qing Zhao, Tong Han, Fan Ding, Xin Jin, Manglai Gao. Selective capture of silver ions from aqueous solution by series of azole derivatives-functionalized silica nanosheets[J]. 中国化学工程学报, 2023, 57(5): 319-328.
[15]	Feng Pan, Sugang Ma, Yu Ge, Chuanlin Fan, Qingshan Zhu. Fluidization thermal decomposition of sodium fluosilicate[J]. 中国化学工程学报, 2023, 57(5): 329-337.

Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production

Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价