SCI和EI收录∣中国化工学会会刊

›› 2010, Vol. 18 ›› Issue (5): 750-754.

• • 上一篇    下一篇

Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F

沈剑, 何潮洪   

  1. State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang Uni versity, Hangzhou 310027, China
  • 收稿日期:2010-01-28 修回日期:2010-06-15 出版日期:2010-10-28 发布日期:2010-10-28
  • 通讯作者: HE Chaohong,E-mail:chhezju@zju.edu.cn
  • 基金资助:
    Supported by the National Natural Science Foundation of China (20576113);Zhejiang Provincial Natural Science Foundation of China (R4090358)

Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F

SHEN Jian, HE Chaohong   

  1. State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang Uni versity, Hangzhou 310027, China
  • Received:2010-01-28 Revised:2010-06-15 Online:2010-10-28 Published:2010-10-28
  • Supported by:
    Supported by the National Natural Science Foundation of China (20576113);Zhejiang Provincial Natural Science Foundation of China (R4090358)

摘要: Triptolide is an important active component of Tripterygium wilfordii Hook F (TWHF) and possesses anti-inflammatory, immunosuppressive, male anti-fertility, and anticancer properties. A new method combining different techniques, including solid-liquid extraction, liquid-liquid partition, column chromatography and high-speed counter-current chromatography (HSCCC) but avoiding the use of chloroform, was developed for the isolation and purification of triptolide from the leaves of TWHF. 48 mg of triptolide at 96.5% purity was obtained from 1 kg of air-dried leaves of TWHF.

关键词: Tripterygium wilfordii Hook F, triptolide, high-speed counter-current chromatography

Abstract: Triptolide is an important active component of Tripterygium wilfordii Hook F (TWHF) and possesses anti-inflammatory, immunosuppressive, male anti-fertility, and anticancer properties. A new method combining different techniques, including solid-liquid extraction, liquid-liquid partition, column chromatography and high-speed counter-current chromatography (HSCCC) but avoiding the use of chloroform, was developed for the isolation and purification of triptolide from the leaves of TWHF. 48 mg of triptolide at 96.5% purity was obtained from 1 kg of air-dried leaves of TWHF.

Key words: Tripterygium wilfordii Hook F, triptolide, high-speed counter-current chromatography