SCI和EI收录∣中国化工学会会刊

›› 2010, Vol. 18 ›› Issue (5): 863-869.

• • 上一篇    下一篇

Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses:Potential and Challenges

张翀, 邢新会   

  1. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
  • 收稿日期:2010-04-26 修回日期:2010-08-16 出版日期:2010-10-28 发布日期:2010-10-28
  • 通讯作者: XING Xinhui,E-mail:xhxing@tsinghua.edu.cn
  • 基金资助:
    Supported by the National Natural Science Foundation of China (20836004, 20806046);the Special Fund for Major State Basic Research Program of China (2009CB724702);the National High Technology Research and Development Program ofChina (2009AA062903)

Fluorescent Proteins as a Visible Molecular Signal for Rapid Quantification of Bioprocesses:Potential and Challenges

ZHANG Chong, XING Xinhui   

  1. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China
  • Received:2010-04-26 Revised:2010-08-16 Online:2010-10-28 Published:2010-10-28
  • Supported by:
    Supported by the National Natural Science Foundation of China (20836004, 20806046);the Special Fund for Major State Basic Research Program of China (2009CB724702);the National High Technology Research and Development Program ofChina (2009AA062903)

摘要: Green fluorescent protein (GFP) and its variants/homolog proteins are generally called as GFP-like fluorescent proteins (FPs), which are widely used as visible molecular tools for monitoring a wide range of biological processes due to their capability of simple, accurate and real time quantification. The FPs-based molecular and visible quantification tools are giving more impact on bioprocess engineering, enabling the biomolecule-level dynamic information to be linked with the process-level events. In this review, different applications of FPs in biological engineering with emphasis on rapid molecular bioprocess quantification, such as quantification of the transcription efficiency, the protein production, the protein folding efficiency, the cell concentration, the intracellular microenvironments and so on, would be first introduced. The challenges of using FPs with respect to actual bioprocess applications for the precise quantification including the interaction of FPs and the fused partner proteins, the maturation of FPs, the inner filter effect and sensing technology were then discussed. Finally, the future development for the FPs used in molecular bioprocess quantification would be proposed.

关键词: green fluorescent protein, fluorescent proteins, bioprocess engineering, quantification, marker

Abstract: Green fluorescent protein (GFP) and its variants/homolog proteins are generally called as GFP-like fluorescent proteins (FPs), which are widely used as visible molecular tools for monitoring a wide range of biological processes due to their capability of simple, accurate and real time quantification. The FPs-based molecular and visible quantification tools are giving more impact on bioprocess engineering, enabling the biomolecule-level dynamic information to be linked with the process-level events. In this review, different applications of FPs in biological engineering with emphasis on rapid molecular bioprocess quantification, such as quantification of the transcription efficiency, the protein production, the protein folding efficiency, the cell concentration, the intracellular microenvironments and so on, would be first introduced. The challenges of using FPs with respect to actual bioprocess applications for the precise quantification including the interaction of FPs and the fused partner proteins, the maturation of FPs, the inner filter effect and sensing technology were then discussed. Finally, the future development for the FPs used in molecular bioprocess quantification would be proposed.

Key words: green fluorescent protein, fluorescent proteins, bioprocess engineering, quantification, marker