Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

›› 2010, Vol. 18 ›› Issue (6): 1013-1017.

Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

刘杰凤^1,2, 任意然¹, 姚善泾¹

1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2. Department of Biotechnology and Food Engineering, Maoming College, Maoming 525000, China

收稿日期:2010-04-29 修回日期:2010-07-16 出版日期:2010-12-28 发布日期:2010-12-28
通讯作者: YAO Shanjing, E-mail: yaosj@zju.edu.cn
基金资助:
Supported by the National Basic Research Program of China(2007CB707805);the National Natural Science Foundation of China(20876139)

Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

LIU Jiefeng^1,2, REN Yiran¹, YAO Shanjing¹

1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2. Department of Biotechnology and Food Engineering, Maoming College, Maoming 525000, China

Received:2010-04-29 Revised:2010-07-16 Online:2010-12-28 Published:2010-12-28
Supported by:
Supported by the National Basic Research Program of China(2007CB707805);the National Natural Science Foundation of China(20876139)

摘要/Abstract

摘要： In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were prepared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule’s membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD₅₀₀),which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.

关键词: microencapsulation, Monascus purpureus, natural pigment, polyelectrolyte complex, NaCS-PDMDAAC capsule, cell immobilization

Abstract: In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were prepared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule’s membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD₅₀₀),which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.

Key words: microencapsulation, Monascus purpureus, natural pigment, polyelectrolyte complex, NaCS-PDMDAAC capsule, cell immobilization

刘杰凤, 任意然, 姚善泾. Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production[J]. , 2010, 18(6): 1013-1017.

LIU Jiefeng, REN Yiran, YAO Shanjing. Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production[J]. , 2010, 18(6): 1013-1017.

参考文献

1 Park, J.K., Chang, H.N., “Microencapsulation of microbial cells”, Biotechnol. Adv., 18, 303-319(2000).
2 Dautzenberg, H., Schuldt, U., Lerche, D., Woehledke, H., Ehwald, R., “Size exclusion properties of polyelectrolyte complex microcapsules prepared from sodium cellulose sulphate and poly [dimethyldiallylammonium chloride]”, J. Membr. Sci., 162, 165-171(1999).
3 Chen, G., Yao, S.J., Guan, Y.X., Lin, D.Q., “Preparation and characterization of NaCS-CMC/PDMDAAC capsules”, Colloids Surf. B Biointerf., 45, 136-143(2005).
4 Yao, S.J., Guan, Y.X, Lin, D.Q., “Mass transfer behaviors of solutes in NaCS-PDMDAAC capsules”, Ind. Eng. Chem. Res., 45(5), 1811-1816(2006).
5 Zhang, J., Yao, S.J., Guan, Y.X., “Preparation of macroporous NaCS-PDADMAC capsule and its characteristics”, J. Membr. Sci., 255(1/2), 89-98(2005).
6 Zhang, J., Guan, Y.X., Ji, Z., Ya, S.J., “Effects on membrane properties of NaCS-PDMDAAC capsules by adding inorganic salts”, J. Membr. Sci., 277, 270-276(2006).
7 Li, M.M., Yao, S.J., “Preparation of polyelectrolyte complex membranes based on sodium cellulose sulfate and poly(dimethyldiallylammonium chloride) and its permeability properties”, J. Appl. Polym. Sci., 112(1), 402-409(2009).
8 Yao, S.J., Cho, M.G., “Diffusion characteristics in microcapsules”, Chin. J. Chem. Eng., 6(2), 116-123(1998).
9 Mei, L.H., Yao, S.J., “Cultivation and modeling of encapsulated Saccharomyces cerevisiae in NaCS-PDMDAAC polyelectrolyte complexes”, J. Microencapsul., 19(4), 397-405(2002).
10 Zhao, Y.N., Chen, G., Yao, S.J., “Microbial production of 1,3-propanediol from glycerol by encapsulated Klebsiella pneumoniae”, Biochem. Eng. J., 32, 93-99(2006).
11 Chen, G., Shen, D.Q., Zhang, G.Y., Fang, B.S., Yao, S.J., “Study on the immobilization of Candida krusei in microcapsule system”, Food and Fermentation Industries, 33(5), 13-16(2007).(in Chinese)
12 Fabre, C.E., Santerre, A.L., Lore, M.O., “Production and food application of the red pigments of Monascus ruber”, J. Food Sci., 58, 1099-1102(1993).
13 Lee, Y.K., Chen, D.C., Chauvatcharin, S., “Production of Monascus pigments by a solid-liquid state culture method”, J. Fermentation Bioeng., 79, 516-518(1995).
14 Lin, C.F., “Isolation and cultural condition of Monascus sp. for the production of pigment in a submerged culture”, J. Fermentation Tech., 51, 407-414(1973).
15 Evans, P.J., Wang, H.Y., “Pigment production from immobilized Monascus sp. utilizing polymeric resin adsorption”, Appl. Environ. Microbiol., 47, 1323-1326(1984).
16 Lin, T.F., Demain, A.L., “Effect of nutrition of Monascus sp. on formation of red pigment”, Appl. Microbiol. Biotechnol., 36, 70-75(1991).
17 Yao, S.J., “An improved process for the preparation of sodium cellulose sulphate”, Chem. Eng. J., 78, 199-204(2000).
18 Zhang, Z.R., Yao, S.J., “Cultivation of encapsulated Monascus purpureus in NaCS-PDMDAAC capsules”, Food and Fermentation Industries, 11, 1-4(2003).(in Chinese)
19 Hamdi, M., Blanc, P.J., Goma, G., “Effect of aeration conditions on the production of red pigments by Monascus purpureus growth on prickly pear juice”, Process Biochem., 31, 543-547(1996).
20 Alteriis, E., Zueco, J., Rico, H., Sentandreu, R., Parascandola, P., “Electrophoretic mobility of external invertase from free and gel-immobilized yeast cells”, Res. Microbiol., 146, 217-225(1995).
21 Park, J.K., Jeong, G.S., Chang, H.N., “The effect of oxygen transfer on the activity of encapsulated whole cell β-galactosidase”, Bioprocess Eng., 17, 197-202(1997).
22 Shuler, M.L., Kargi, F., Bioprocess Engineering: Basic Concepts, Prentice-Hall, New Jersey(1992).
23 Mansfeld, J., Forster, M., Hoffmann, T., Schellenberger, A., “Coimmobilization of Yarrowia lipolytica cells and invertase in polyelectrolyte complex microcapsules”, Enzyme Microb. Technol., 17, 11-17(1995).

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Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

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