Comparison of Antioxidant and Antimicrobial Activities of Methanolic Extracts of the Artemisia sp. Recovered by Different Extraction Techniques

Chinese Journal of Chemical Engineering ›› 2011, Vol. 19 ›› Issue (3): 504-511.

• 催化、动力学与反应工程 • 上一篇下一篇

Comparison of Antioxidant and Antimicrobial Activities of Methanolic Extracts of the Artemisia sp. Recovered by Different Extraction Techniques

Ivana Karabegovi?¹, Milena Nikolova², Dragan Veli?kovi?³, Sаša Stoji?evi?¹, Vlada Veljkovi?¹, Miodrag Lazi?¹

1. Faculty of Technology, 124 Bulevar oslobodjenja St., 16000 Leskovac, Serbia;
2. Institute of Botany, Bulgarian Academy of Sciences, 23 G.Bonchev St., 1113 Sofia, Bulgaria;
3. College of Agriculture and Food Technology, 1?irila i Metodija St., 18400 Prokuplje, Serbia

收稿日期:2009-06-02 修回日期:2011-01-20 出版日期:2011-06-28 发布日期:2011-06-28
通讯作者: Ivana Karabegović, E-mail: icaneca@gmail.com
基金资助:
Supported by the Ministry of Science and Environmental Protection,Republic of Serbia(172047)

Comparison of Antioxidant and Antimicrobial Activities of Methanolic Extracts of the Artemisia sp. Recovered by Different Extraction Techniques

Ivana Karabegovi?¹, Milena Nikolova², Dragan Veli?kovi?³, Saša Stoji?evi?¹, Vlada Veljkovi?¹, Miodrag Lazi?¹

1. Faculty of Technology, 124 Bulevar oslobodjenja St., 16000 Leskovac, Serbia;
2. Institute of Botany, Bulgarian Academy of Sciences, 23 G.Bonchev St., 1113 Sofia, Bulgaria;
3. College of Agriculture and Food Technology, 1?irila i Metodija St., 18400 Prokuplje, Serbia

Received:2009-06-02 Revised:2011-01-20 Online:2011-06-28 Published:2011-06-28
Supported by:
Supported by the Ministry of Science and Environmental Protection,Republic of Serbia(172047)

摘要/Abstract

摘要： The polyphenol content,antioxidant and antimicrobial activities of the extracts obtained by classical, ultrasonic and Soxhlet extractions from dry aerial parts of two Artemisia species(Artemisia vulgaris and Artemisia campestris)were compared.Ultrasound positively affected the yield of extractive substance and the kinetics of extraction,but the extract obtained by the classical extraction showed the highest antioxidant activities and contained higher total contents of phenolic compounds and flavonoids than the extracts obtained by two other extraction techniques.Both flavonoid aglycones(apigenin,quercetin,quercetin 3,3′-dimethyl ether)and flavonoid glycosides(rutin,hyperoside and kaempferol 3-rhamnoside)were identified by thin layer chromatograph(TLC)analysis in the extracts from both species.A.campestris extracts were richer in quercetin than A.vulgaris and its antimicrobial activity was also better than A.vulgaris.Extracts obtained from both species were found to be more effective on the tested yeasts than bacteria.The kinetics of the total extractive substances,such as phenolic,flavonoids and quercetin extraction,was successfully described by the model of unsteady-state diffusion.

关键词: polyphenol content, antioxidant activity, antimicrobial activity, extraction, flavonoids, Artemisia vulgaris, Artemisia campestris

Abstract: The polyphenol content,antioxidant and antimicrobial activities of the extracts obtained by classical, ultrasonic and Soxhlet extractions from dry aerial parts of two Artemisia species(Artemisia vulgaris and Artemisia campestris)were compared.Ultrasound positively affected the yield of extractive substance and the kinetics of extraction,but the extract obtained by the classical extraction showed the highest antioxidant activities and contained higher total contents of phenolic compounds and flavonoids than the extracts obtained by two other extraction techniques.Both flavonoid aglycones(apigenin,quercetin,quercetin 3,3′-dimethyl ether)and flavonoid glycosides(rutin,hyperoside and kaempferol 3-rhamnoside)were identified by thin layer chromatograph(TLC)analysis in the extracts from both species.A.campestris extracts were richer in quercetin than A.vulgaris and its antimicrobial activity was also better than A.vulgaris.Extracts obtained from both species were found to be more effective on the tested yeasts than bacteria.The kinetics of the total extractive substances,such as phenolic,flavonoids and quercetin extraction,was successfully described by the model of unsteady-state diffusion.

Key words: polyphenol content, antioxidant activity, antimicrobial activity, extraction, flavonoids, Artemisia vulgaris, Artemisia campestris

Ivana Karabegović, Milena Nikolova, Dragan Veličković, Sаša Stojičević, Vlada Veljković, Miodrag Lazić. Comparison of Antioxidant and Antimicrobial Activities of Methanolic Extracts of the Artemisia sp. Recovered by Different Extraction Techniques[J]. Chinese Journal of Chemical Engineering, 2011, 19(3): 504-511.

Ivana Karabegović, Milena Nikolova, Dragan Veličković, Saša Stojičević, Vlada Veljković, Miodrag Lazić. Comparison of Antioxidant and Antimicrobial Activities of Methanolic Extracts of the Artemisia sp. Recovered by Different Extraction Techniques[J]. Chin.J.Chem.Eng., 2011, 19(3): 504-511.

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Comparison of Antioxidant and Antimicrobial Activities of Methanolic Extracts of the Artemisia sp. Recovered by Different Extraction Techniques

Comparison of Antioxidant and Antimicrobial Activities of Methanolic Extracts of the Artemisia sp. Recovered by Different Extraction Techniques

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