Medium Optimization for Improved Ethanol Production in Very High Gravity Fermentation

›› 2011, Vol. 19 ›› Issue (6): 1017-1022.

Medium Optimization for Improved Ethanol Production in Very High Gravity Fermentation

胡纯铿, 秦晴, 高培培

Department of Biological Engineering, School of Chemical Engineering, Huaqiao University, Xiamen 361021, China

收稿日期:2010-07-26 修回日期:2011-07-28 出版日期:2011-12-28 发布日期:2012-04-24
通讯作者: HU Chunkeng, E-mail: ckhu@hqu.edu.cn
基金资助:
Supported by the Natural Science Foundation of Fujian Province of China (E0810018)

Medium Optimization for Improved Ethanol Production in Very High Gravity Fermentation

HU Chunkeng, QIN Qing, GAO Peipei

Department of Biological Engineering, School of Chemical Engineering, Huaqiao University, Xiamen 361021, China

Received:2010-07-26 Revised:2011-07-28 Online:2011-12-28 Published:2012-04-24
Supported by:
Supported by the Natural Science Foundation of Fujian Province of China (E0810018)

摘要/Abstract

摘要： An optimal medium (300 g·L^-1 initial glucose) comprising 6.3 mmol·L^-1 Mg²⁺, 5.0 mmol·L^-1 Ca²⁺, 15.0 g·L^-1 peptone and 21.5 g·L^-1 yeast extract was determined by uniform design to improve very high gravity (VHG) ethanol fermentation, showing over 30% increase in final ethanol (from 13.1% to 17.1%, by volume), 29% decrease in fermentation time (from 84 to 60 h), 80% increase in biomass formation and 26% increase in glucose utilization. Experiments also revealed physiological aspects linked to the fermentation enhancements. Compared to the control, trehalose in the cells grown in optimal fermentation medium increased 17.9-, 2.8-, 1.9-, 1.8- and 1.9-fold at the fermentation time of 12, 24, 36, 48 and 60 h, respectively. Its sharp rise at the early stage of fermentation when there was a considerable osmotic stress suggested that trehalose played an important role in promoting fermentation. Meanwhile, at the identical five fermentation time, the plasma membrane ATPase activity of the cells grown in optimal medium was 2.3, 1.8, 1.6, 1.5 and 1.3 times that of the control, respectively. Their disparities in enzymatic activity became wider when the glucose levels were dramatically changed for ethanol production, suggesting this enzyme also contributed to the fermentation improvements. Thus, medium optimization for VHG ethanol fermentation was found to trigger the increased yeast trehalose accumulation and plasma membrane ATPase activity.

关键词: medium optimization, very high gravity fermentation, trehalose, plasma membrane ATPase

Abstract: An optimal medium (300 g·L^-1 initial glucose) comprising 6.3 mmol·L^-1 Mg²⁺, 5.0 mmol·L^-1 Ca²⁺, 15.0 g·L^-1 peptone and 21.5 g·L^-1 yeast extract was determined by uniform design to improve very high gravity (VHG) ethanol fermentation, showing over 30% increase in final ethanol (from 13.1% to 17.1%, by volume), 29% decrease in fermentation time (from 84 to 60 h), 80% increase in biomass formation and 26% increase in glucose utilization. Experiments also revealed physiological aspects linked to the fermentation enhancements. Compared to the control, trehalose in the cells grown in optimal fermentation medium increased 17.9-, 2.8-, 1.9-, 1.8- and 1.9-fold at the fermentation time of 12, 24, 36, 48 and 60 h, respectively. Its sharp rise at the early stage of fermentation when there was a considerable osmotic stress suggested that trehalose played an important role in promoting fermentation. Meanwhile, at the identical five fermentation time, the plasma membrane ATPase activity of the cells grown in optimal medium was 2.3, 1.8, 1.6, 1.5 and 1.3 times that of the control, respectively. Their disparities in enzymatic activity became wider when the glucose levels were dramatically changed for ethanol production, suggesting this enzyme also contributed to the fermentation improvements. Thus, medium optimization for VHG ethanol fermentation was found to trigger the increased yeast trehalose accumulation and plasma membrane ATPase activity.

Key words: medium optimization, very high gravity fermentation, trehalose, plasma membrane ATPase

胡纯铿, 秦晴, 高培培. Medium Optimization for Improved Ethanol Production in Very High Gravity Fermentation[J]. , 2011, 19(6): 1017-1022.

HU Chunkeng, QIN Qing, GAO Peipei. Medium Optimization for Improved Ethanol Production in Very High Gravity Fermentation[J]. , 2011, 19(6): 1017-1022.

参考文献

1 Zaldivar, J., Nielsen, J., Olsson, L., “Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration”, Appl. Microbiol. Biotechnol., 56, 17-34(2001).
2 Thomas, K.C., Ingledew, W.M., “Fuel alcohol production: effects of free amino nitrogen on fermentation of very-high-gravity wheat mashes”, Appl. Environ. Microbiol., 56, 2046-2050(1990).
3 Bayrock, D.P., Ingledew, W.M., “Application of multistage continuous fermentation for production of fuel alcohol by very-high-gravity fermentation technology”, J. Ind. Microbiol. Biotechnol., 27, 87-93(2001).
4 Bai, F.W., Anderson, W.A., Moo-Young, M., “Ethanol fermentation technologies from sugar and starch feedstocks”, Biotechnol. Adv., 26, 89-105(2008).
5 Alfenore, S., Molina-Jouve, C., Guillouet, S.E., Uribelarrea, J.L., Goma, G., Benbadis, L., “Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process”, Appl. Microbiol. Biotechnol., 60, 67-72(2002).
6 Bafrncova, P., Smogrovicova, D., Slavikova, I., Patkova, J., Domeny, Z., “Improvement of very high gravity ethanol fermentation by media supplementation using Saccharomyces cerevisiae”, Biotechnol. Lett., 21, 337-341(1999).
7 Jones, A.M., Ingledew, W.M., “Fuel alcohol production: appraisal of nitrogenous yeast foods for very high gravity wheat mash fermentation”, Proc. Biochem., 29, 483-488(1994).
8 Reddy, L.V.A., Reddy, O.V.S., “Improvement of ethanol production in very high gravity fermentation by horse gram(Dolichos biflorus) flour supplementation”, Lett. Appl. Mircrobiol., 41, 440-444(2005).
9 Caridi, A., “Protective agents used to reverse the metabolic changes induced in wine yeasts by concomitant osmotic and thermal stress”, Lett. Appl. Microbiol., 35, 98-101(2002).
10 Hu, C.K., Bai, F.W., An, L.J., “Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg²⁺ via reduction in plasma membrane permeability”, Biotechnol. Lett., 25, 1191-1194(2003).
11 Wang, B.G., Chemical Process Synthesis Experiments, Tsinghua University Press, Beijing(2004).
12 Serrano, R., “Structure, function and regulation of plasma membrane H+-ATPase”, FEBS Lett., 325, 108-111(1993).
13 Hu, C.K., Bai, F.W., An, L.J., “Enhancements in ethanol tolerance of a self-flocculating yeast by Ca 2+ through decrease in plasmalemma permeability”, Chin. J. Biotechnol., 19, 715-719(2003).
14 Tang, Q.Y., Feng, M.G., DPS Data Processing System for Practical Statistics, Science Press, Beijing(2002).
15 Zhao, Q.Y., Zhang, W., Jin, M.F., Yu, X.J., Deng, M.C., “Formulation of a basal medium for primary cell culture of the marine sponge Hymeniacidon perleve”, Biotechnol. Prog., 21, 1008-1012(2005).
16 Wang, F.Q., Gao, J., Yang, C.Y., Xu, P., “Optimization of an ethanol production medium in very high gravity fermentation”, Biotechnol. Lett., 29, 233-236(2007).
17 Bandara, A., Fraser, S., Chambers P.J., Stanley G.A., “Trehalose promotes the survival of Saccharomyces cerevisiae during lethal ethanol stress, but does not influence growth under sublethal ethanol stress”, FEMS Yeast Res., 9, 1208-1216(2009).
18 Conlin, L.K., Nelson, H.C.M., “The natural osmolyte trehalose is a positive regulator of the heat-induced activity of yeast heat shock transcription factor”, Mol. Cell. Biol., 27, 1505-1515(2007).
19 Pham, T.K., Wright, P.C., “The proteomic response of Saccharomyces cerevisiae in very high glucose conditions with amino acid supplementation”, J. Proteome Res., 7, 4766-4774(2008).
20 Goossens, A., de la Fuente, N., Forment, J., Serrano, R., Portillo, F., “Regulation of yeast H⁺-ATPase by protein kinases belonging to a family dedicated to activation of plasma membrane transporters”, Mol. Cell. Biol., 20, 7654-7661(2000).
21 McCusker, J.H., Perlin, D.S., Haber, J.E., “Pleiotrophic plasma membrane ATPase mutations of Saccharomyces cerevisiae”, Mol. Cell. Biol., 7, 4082-4088(1987).
22 Monteiro, G.A., Sá-Correia, I., “In vivo activation of yeast plasma membrane H⁺-ATPase by ethanol: effect on the kinetic parameters and involvement of the carboxyl-terminus regulatory domain”, Biochim. Biophys. Acta, 1370, 310-316(1998).
23 Teixeira, M.C., Raposo, L.R., Mira, N.P., Lourenco, A.B., Sá-Correia, I., “Genome-wide identification of Saccharomyces cerevisiae genes required for maximal tolerance to ethanol”, Appl. Environ. Microbiol., 75, 5761-5772(2009).
24 Portillo, F., “Regulation of plasma membrane H⁺-ATPase in fungi and plants”, Biochim. Biophys. Acta, 1469, 31-42(2000).
25 Hohmann, S., “Osmotic stress signaling and osmoadaptation in yeasts”, Microbiol. Mol. Biol. Rev., 66, 300-372(2002).
26 Teixeira, M.C., Raposo, L.R., Palma, M., Sá-Correia, I., “Identification of genes required for maximal tolerance to high-glucose concentrations, as those present in industrial alcoholic fermentation media, through a chemogenomics approach”, OMICS, 14, 201-210(2010).

Medium Optimization for Improved Ethanol Production in Very High Gravity Fermentation

Medium Optimization for Improved Ethanol Production in Very High Gravity Fermentation

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