Enhanced cold active lipase production by metagenomic library recombinant clone CALIP3 with a step-wise temperature and dissolved oxygen level control strategy

›› 2016, Vol. 24 ›› Issue (9): 1263-1269.

• Biotechnology and Bioengineering • 上一篇下一篇

Enhanced cold active lipase production by metagenomic library recombinant clone CALIP3 with a step-wise temperature and dissolved oxygen level control strategy

Zhuhua Chan¹, RunpingWang¹, Fan Yang¹, Runying Zeng^1,2

1 State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, SOA, Xiamen 361005, China;
2 South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510000, China

收稿日期:2015-06-25 修回日期:2016-03-31 出版日期:2016-09-28 发布日期:2016-11-11
通讯作者: Runying Zeng a,Third Institute of Oceanography,SOA,178 Daxue road,Xiamen 361005,China. Tel./Fax:+865922195323.E-mail address:zeng@tio.org.cn
基金资助:
Supported by the Hi-Tech Research and Development Program of China (863 program of China; 2012AA092103), China Ocean Mineral Resources R&D Association (DY125-15-T-06).

Enhanced cold active lipase production by metagenomic library recombinant clone CALIP3 with a step-wise temperature and dissolved oxygen level control strategy

Zhuhua Chan¹, RunpingWang¹, Fan Yang¹, Runying Zeng^1,2

1 State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, SOA, Xiamen 361005, China;
2 South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510000, China

Received:2015-06-25 Revised:2016-03-31 Online:2016-09-28 Published:2016-11-11
Supported by:
Supported by the Hi-Tech Research and Development Program of China (863 program of China; 2012AA092103), China Ocean Mineral Resources R&D Association (DY125-15-T-06).

摘要/Abstract

摘要： A metagenomic library recombinant clone CAPL3, an Escherichia coli strain generated by transformed with metagenomic library from deep-sea sediments, can efficiently produce cold active lipase. The effects of both temperature and dissolved oxygen (DO) on cold active lipase production by batch culture of metagenomic library recombinant clone (CAPL3) from deep-sea sediment were investigated. First, a two-stage temperature control strategy was developed, in which the temperature was kept at 34℃ for the first 15 h, and then switched to 30℃. The cold active lipase activity and productivity reached 315.2 U·ml^-1 and 8.08 U·ml^-1·h^-1, respectively, increased by both 14.5% compared to the results obtained with temperature controlled at 30℃. In addition, different DO control modes were conducted, based on the data obtained from the different DO control strategies and analysis of kinetics parameters at different DO levels. A step-wise temperature and DO control strategy were developed to improve lipase production, i.e., temperature and DO level were controlled at 34℃, 30% during 0-15 h; 30℃, 30% during 15-18 h, and 30℃, 20% during 18-39 h. With this strategy, the maximum lipase activity reached 354.6 U·ml^-1 at 39 h, which was 28.8% higher than that achieved without temperature and DO control (275.3 U·ml^-1).

关键词: Deep-sea sediments, Metagenomic library, Cold active lipase, Step-wise temperature and DO control strategy

Abstract: A metagenomic library recombinant clone CAPL3, an Escherichia coli strain generated by transformed with metagenomic library from deep-sea sediments, can efficiently produce cold active lipase. The effects of both temperature and dissolved oxygen (DO) on cold active lipase production by batch culture of metagenomic library recombinant clone (CAPL3) from deep-sea sediment were investigated. First, a two-stage temperature control strategy was developed, in which the temperature was kept at 34℃ for the first 15 h, and then switched to 30℃. The cold active lipase activity and productivity reached 315.2 U·ml^-1 and 8.08 U·ml^-1·h^-1, respectively, increased by both 14.5% compared to the results obtained with temperature controlled at 30℃. In addition, different DO control modes were conducted, based on the data obtained from the different DO control strategies and analysis of kinetics parameters at different DO levels. A step-wise temperature and DO control strategy were developed to improve lipase production, i.e., temperature and DO level were controlled at 34℃, 30% during 0-15 h; 30℃, 30% during 15-18 h, and 30℃, 20% during 18-39 h. With this strategy, the maximum lipase activity reached 354.6 U·ml^-1 at 39 h, which was 28.8% higher than that achieved without temperature and DO control (275.3 U·ml^-1).

Key words: Deep-sea sediments, Metagenomic library, Cold active lipase, Step-wise temperature and DO control strategy

中图分类号:

10.1016/j.cjche.2016.04.033

Zhuhua Chan, RunpingWang, Fan Yang, Runying Zeng. Enhanced cold active lipase production by metagenomic library recombinant clone CALIP3 with a step-wise temperature and dissolved oxygen level control strategy[J]. , 2016, 24(9): 1263-1269.

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Enhanced cold active lipase production by metagenomic library recombinant clone CALIP3 with a step-wise temperature and dissolved oxygen level control strategy

Enhanced cold active lipase production by metagenomic library recombinant clone CALIP3 with a step-wise temperature and dissolved oxygen level control strategy

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