Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity

doi:10.1016/j.cjche.2019.11.012

中国化学工程学报 ›› 2020, Vol. 28 ›› Issue (4): 1120-1128.DOI: 10.1016/j.cjche.2019.11.012

• Biotechnology and Bioengineering • 上一篇下一篇

Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity

Linian Cai¹, Shengnan Xu¹, Tao Lu², Dongqiang Lin¹, Shanjing Yao¹

1 Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 College of Environment, Zhejiang University of Technology, Hangzhou 310014, China

收稿日期:2019-10-10 修回日期:2019-11-21 出版日期:2020-04-28 发布日期:2020-07-27
通讯作者: Linian Cai, Shengnan Xu, Tao Lu, Dongqiang Lin, Shanjing Yao
基金资助:
This work was supported by National Natural Science Foundation of China (21576233, 21878263) and Fundamental Research Funds for the Central Universities.

Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity

Linian Cai¹, Shengnan Xu¹, Tao Lu², Dongqiang Lin¹, Shanjing Yao¹

1 Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 College of Environment, Zhejiang University of Technology, Hangzhou 310014, China

Received:2019-10-10 Revised:2019-11-21 Online:2020-04-28 Published:2020-07-27
Contact: Linian Cai, Shengnan Xu, Tao Lu, Dongqiang Lin, Shanjing Yao
Supported by:
This work was supported by National Natural Science Foundation of China (21576233, 21878263) and Fundamental Research Funds for the Central Universities.

摘要/Abstract

摘要： The cellulase cocktail produced by marine Aspergillus niger exhibits a property of salt-tolerance, which is of great potential in cellulose degradation in high salt environment. In order to explain the mechanism on the salttolerance of the cellulase cocktail produced by marine A. niger, six cellulase components (AnCel6, AnCel7A, AnCel7B, AnEGL, AnBGL1 and AnBGL2) were obtained by directed expression. Studies on their enzymatic properties revealed that one β-glucosidase (AnBGL2) and one endoglucanase (AnEGL) exhibited an outstanding salttolerant property, and one cellobiohydrolase (AnCel7B) exhibited a certain salt-tolerant property. Subsequent study revealed that the salt-tolerant AnEGL and AnCel7B endowed the cellulase cocktail with stronger salttolerant property, while the salt-tolerant AnBGL2 had no positive effect. Moreover, after overexpression of AnCel6, AnCel7A, AnCel7B and AnEGL, the activity of cellulase cocktail increased by 80%, 70%, 63% and 68%, respectively. However, the activity of cellulase cocktail was not improved after overexpression of AnBGL1 and AnBGL2. After mixed-strain fermentation with cellobiohydrolase recombinants (cel6a, cel7a and cel7b recombinants) and endoglucanase recombinant (egl recombinant), the the activity of cellulase cocktail increased by 114%, 102% and 91%, respectively.

关键词: Marine Aspergillus niger, Cellulase component, Directed expression, Salt-tolerance, Cellulase activity

Abstract: The cellulase cocktail produced by marine Aspergillus niger exhibits a property of salt-tolerance, which is of great potential in cellulose degradation in high salt environment. In order to explain the mechanism on the salttolerance of the cellulase cocktail produced by marine A. niger, six cellulase components (AnCel6, AnCel7A, AnCel7B, AnEGL, AnBGL1 and AnBGL2) were obtained by directed expression. Studies on their enzymatic properties revealed that one β-glucosidase (AnBGL2) and one endoglucanase (AnEGL) exhibited an outstanding salttolerant property, and one cellobiohydrolase (AnCel7B) exhibited a certain salt-tolerant property. Subsequent study revealed that the salt-tolerant AnEGL and AnCel7B endowed the cellulase cocktail with stronger salttolerant property, while the salt-tolerant AnBGL2 had no positive effect. Moreover, after overexpression of AnCel6, AnCel7A, AnCel7B and AnEGL, the activity of cellulase cocktail increased by 80%, 70%, 63% and 68%, respectively. However, the activity of cellulase cocktail was not improved after overexpression of AnBGL1 and AnBGL2. After mixed-strain fermentation with cellobiohydrolase recombinants (cel6a, cel7a and cel7b recombinants) and endoglucanase recombinant (egl recombinant), the the activity of cellulase cocktail increased by 114%, 102% and 91%, respectively.

Key words: Marine Aspergillus niger, Cellulase component, Directed expression, Salt-tolerance, Cellulase activity

Linian Cai, Shengnan Xu, Tao Lu, Dongqiang Lin, Shanjing Yao. Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity[J]. 中国化学工程学报, 2020, 28(4): 1120-1128.

Linian Cai, Shengnan Xu, Tao Lu, Dongqiang Lin, Shanjing Yao. Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity[J]. Chinese Journal of Chemical Engineering, 2020, 28(4): 1120-1128.

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Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity

Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity

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