Insights into constructing a stable and efficient microbial consortium system

doi:10.1016/j.cjche.2024.08.003

中国化学工程学报 ›› 2024, Vol. 76 ›› Issue (12): 95-104.DOI: 10.1016/j.cjche.2024.08.003

Insights into constructing a stable and efficient microbial consortium system

Yinshan Lin¹, Haohong Lin¹, Jingyuan Liu¹, Fengxue Xin^1,2, Minjiao Chen¹, Weiliang Dong^1,2, Xiujuan Qian¹, Min Jiang^1,2

1. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China;
2. State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211800, China

收稿日期:2024-06-05 修回日期:2024-07-31 接受日期:2024-08-11 出版日期:2024-12-28 发布日期:2024-09-12
通讯作者: Xiujuan Qian,E-mail:xiujuanqian@njtech.edu.cn
基金资助:
This work was supported by the National Key Research and Development Program of China (2022YFC2104800), the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTD2205), and the State Key Laboratory of Materials-Oriented Chemical Engineering (KL-MCE-23A10).

Insights into constructing a stable and efficient microbial consortium system

Yinshan Lin¹, Haohong Lin¹, Jingyuan Liu¹, Fengxue Xin^1,2, Minjiao Chen¹, Weiliang Dong^1,2, Xiujuan Qian¹, Min Jiang^1,2

1. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China;
2. State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211800, China

Received:2024-06-05 Revised:2024-07-31 Accepted:2024-08-11 Online:2024-12-28 Published:2024-09-12
Contact: Xiujuan Qian,E-mail:xiujuanqian@njtech.edu.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2022YFC2104800), the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTD2205), and the State Key Laboratory of Materials-Oriented Chemical Engineering (KL-MCE-23A10).

摘要/Abstract

摘要： The concept of labor division and multi-module cooperation of microbial consortia offers it promising potentials in various areas, such as the utilization of complex substrates, synthesis of natural compounds with long metabolic pathways and remediation of environmental pollutants within a hostile environment. Consequently, synthetic microbial consortia represent a new frontier for synthetic biology because they can solve more complex problems than monocultures. However, current research on microbial consortia often involves the simple mixing of multiphase systems, where strains are co-cultured sequentially or individually cultured and then mixed-cultured. The instability and low efficiency of microbial consortia systems hindered their practical application. To construct a stable and efficient microbial consortium, it is essential to consider the different growth and metabolic characteristics of strains, the competition for various nutrients as well as the complex carbon, energy and signaling dynamics within the system. In this review, we provide a progressive strategy for constructing a stable and efficient microbial consortium system across three stages: compromised stage (work together), microenvironment-oriented stage (work better), and metabolite delivery-enhanced stage (work best). The detailed methods and points for attention of each stage are summarized, with a highlight on the technical bottleneck and application limitations. Through the integration of interdisciplinary strategies, such as materials science and mathematical models, the goal of building a stable and efficient microbial consortium is constantly advanced.

关键词: Microbial consortium, Construction method, Stable and efficient, Progressive strategy, Multiple strategies integration

Abstract: The concept of labor division and multi-module cooperation of microbial consortia offers it promising potentials in various areas, such as the utilization of complex substrates, synthesis of natural compounds with long metabolic pathways and remediation of environmental pollutants within a hostile environment. Consequently, synthetic microbial consortia represent a new frontier for synthetic biology because they can solve more complex problems than monocultures. However, current research on microbial consortia often involves the simple mixing of multiphase systems, where strains are co-cultured sequentially or individually cultured and then mixed-cultured. The instability and low efficiency of microbial consortia systems hindered their practical application. To construct a stable and efficient microbial consortium, it is essential to consider the different growth and metabolic characteristics of strains, the competition for various nutrients as well as the complex carbon, energy and signaling dynamics within the system. In this review, we provide a progressive strategy for constructing a stable and efficient microbial consortium system across three stages: compromised stage (work together), microenvironment-oriented stage (work better), and metabolite delivery-enhanced stage (work best). The detailed methods and points for attention of each stage are summarized, with a highlight on the technical bottleneck and application limitations. Through the integration of interdisciplinary strategies, such as materials science and mathematical models, the goal of building a stable and efficient microbial consortium is constantly advanced.

Key words: Microbial consortium, Construction method, Stable and efficient, Progressive strategy, Multiple strategies integration

Yinshan Lin, Haohong Lin, Jingyuan Liu, Fengxue Xin, Minjiao Chen, Weiliang Dong, Xiujuan Qian, Min Jiang. Insights into constructing a stable and efficient microbial consortium system[J]. 中国化学工程学报, 2024, 76(12): 95-104.

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Insights into constructing a stable and efficient microbial consortium system

Insights into constructing a stable and efficient microbial consortium system

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