SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (9): 1057-1061.DOI: 10.1016/S1004-9541(13)60549-2

• 生物技术与生物工程 • 上一篇    下一篇

Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources

魏晓星1,2, 刘峰2,3, 简嘉2, 王瑞妍2, 陈国强2,4   

  1. 1 Department of Basic Medicine, Medical College of Qinghai University, Xining 810016, China;
    2 MOE Key Lab of Bioinformatics and Systems Biology, Department of Biological Science and Biotechnology, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China;
    3 Tuspark Kunshan Biotech Science Park Development Center, 4/F, Science & Technology Plaza, Tsinghua Science Park, Kunshan, Jiangsu 215347, China;
    4 Center for Nano and Micro Mechanics, Tsinghua University, Beijing 100084, China
  • 收稿日期:2012-09-27 修回日期:2013-02-28 出版日期:2013-09-28 发布日期:2013-10-01
  • 通讯作者: CHEN Guoqiang
  • 基金资助:

    Supported by the National Natural Science Foundation of China (31260015), Natural Science Foundation of Qinghai Province (2012-Z-919Q), the Extramural Project from State Key Laboratory for Agrobiotechnology (2012SKLAB06-5) and the Research Funds for Young Project of Qinghai University (2011-QYY-1)

Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources

WEI Xiaoxing1,2, LIU Feng2,3, JIAN Jia2, WANG Ruiyan2, CHEN Guoqiang2,4   

  1. 1 Department of Basic Medicine, Medical College of Qinghai University, Xining 810016, China;
    2 MOE Key Lab of Bioinformatics and Systems Biology, Department of Biological Science and Biotechnology, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China;
    3 Tuspark Kunshan Biotech Science Park Development Center, 4/F, Science & Technology Plaza, Tsinghua Science Park, Kunshan, Jiangsu 215347, China;
    4 Center for Nano and Micro Mechanics, Tsinghua University, Beijing 100084, China
  • Received:2012-09-27 Revised:2013-02-28 Online:2013-09-28 Published:2013-10-01
  • Supported by:

    Supported by the National Natural Science Foundation of China (31260015), Natural Science Foundation of Qinghai Province (2012-Z-919Q), the Extramural Project from State Key Laboratory for Agrobiotechnology (2012SKLAB06-5) and the Research Funds for Young Project of Qinghai University (2011-QYY-1)

摘要: Synthetic biology promises to simplify the construction of metabolic pathways by assembling the detached modules of the whole pathway. This gives new approaches for the microbial production of industrial products such as polyhydroxyalkanoates (PHA). In this study, to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) by Pseudomonas stutzeri 1317 from unrelated carbon sources such as glucose, the phaC1-phaZ-phaC2 operon of P. stutzeri 1317 was knocked out to generate the PHA deficient mutant P. stutzeri 1317LF. Then three modules containing phaCAhAReBRe, phaCAhBReGPp and phaCAhPAh were introduced into P. stutzeri 1317LF separately. The shake flask results indicated that the precursor supply and PHA synthase activity were the vital factors for the PHBHHx accumulation of P. stutzeri 1317LF. Furthermore, the PHBHHx accumulation of the recombinants from different carbon resources were performed. The highest PHBHHx content was 23.7%(by mass) with 58.6%(by mole) 3HB fraction. These results provide basis for further improving the PHBHHx accumulation of P. stutzeri from unrelated carbon sources.

关键词: Pseudomonas stutzeri 1317, PHBHHx, unrelated carbon sources

Abstract: Synthetic biology promises to simplify the construction of metabolic pathways by assembling the detached modules of the whole pathway. This gives new approaches for the microbial production of industrial products such as polyhydroxyalkanoates (PHA). In this study, to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) by Pseudomonas stutzeri 1317 from unrelated carbon sources such as glucose, the phaC1-phaZ-phaC2 operon of P. stutzeri 1317 was knocked out to generate the PHA deficient mutant P. stutzeri 1317LF. Then three modules containing phaCAhAReBRe, phaCAhBReGPp and phaCAhPAh were introduced into P. stutzeri 1317LF separately. The shake flask results indicated that the precursor supply and PHA synthase activity were the vital factors for the PHBHHx accumulation of P. stutzeri 1317LF. Furthermore, the PHBHHx accumulation of the recombinants from different carbon resources were performed. The highest PHBHHx content was 23.7%(by mass) with 58.6%(by mole) 3HB fraction. These results provide basis for further improving the PHBHHx accumulation of P. stutzeri from unrelated carbon sources.

Key words: Pseudomonas stutzeri 1317, PHBHHx, unrelated carbon sources