A Two-stage pH and Temperature Control with Substrate Feeding Strategy for Production of Gamma-aminobutyric Acid by Lactobacillus brevis CGMCC 1306

doi:10.1016/S1004-9541(13)60568-6

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (10): 1190-1194.DOI: 10.1016/S1004-9541(13)60568-6

• 生物技术与生物工程 • 上一篇

A Two-stage pH and Temperature Control with Substrate Feeding Strategy for Production of Gamma-aminobutyric Acid by Lactobacillus brevis CGMCC 1306

彭春龙¹, 黄俊², 胡升³, 赵伟睿¹, 姚善泾¹, 梅乐和^1,3

1 Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China;
3 School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China

收稿日期:2012-08-29 修回日期:2013-01-24 出版日期:2013-10-28 发布日期:2013-10-29
通讯作者: MEI Lehe
基金资助:
Supported by the National Natural Science Foundation of China (30970638, 21176220 and 31240054), Zhejiang Provincial Natural Science Foundation (Z13B06008) and the National Basic Research Program of China (2007CB714305).

A Two-stage pH and Temperature Control with Substrate Feeding Strategy for Production of Gamma-aminobutyric Acid by Lactobacillus brevis CGMCC 1306

PENG Chunlong¹, HUANG Jun², HU Sheng³, ZHAO Weirui¹, YAO Shanjing¹, MEI Lehe^1,3

1 Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China;
3 School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China

Received:2012-08-29 Revised:2013-01-24 Online:2013-10-28 Published:2013-10-29
Contact: MEI Lehe
Supported by:
Supported by the National Natural Science Foundation of China (30970638, 21176220 and 31240054), Zhejiang Provincial Natural Science Foundation (Z13B06008) and the National Basic Research Program of China (2007CB714305).

摘要/Abstract

摘要： Methods to optimize the production of gamma-aminobutyric acid (GABA) by Lactobacillus brevis CGMCC 1306 were investigated. Results indicated that cell growth was maximal at pH 5.0, while pH 4.5 was preferable to GABA formation. The optimal temperature for cell growth (35 ℃) was lower than that for GABA formation (40 ℃). In a two-stage pH and temperature control fermentation, cultures were maintained at pH 5.0 and 35 ℃ for 32 h, then adjusted to pH 4.5 and 40 ℃, GABA production increased remarkably and reached 474.79 mmol·L^-1 at 72 h, while it was 398.63 mmol·L^-1 with one stage pH and temperature control process, in which cultivation conditions were constantly controlled at pH 5.0 and 35 ℃. In order to avoid the inhibition of cell growth at higher L-monosodium glutamate (L-MSG) concentrations, the two-stage control fermentation with substrate feeding strategy was applied to GABA production, with 106.87 mmol (20 g) L-MSG supplemented into the shaking-flask at 32 h and 56 h post-inoculation separately. The GABA concentration reached 526.33 mmol·L^-1 at 72 h with the fermentation volume increased by 38%. These results will provide primary data to realize large-scale production of GABA by L. brevis CGMCC 1306.

关键词: gamma-aminobutyric acid, Lactobacillus brevis CGMCC 1306, two-stage pH and temperature control, substrate feeding

Abstract: Methods to optimize the production of gamma-aminobutyric acid (GABA) by Lactobacillus brevis CGMCC 1306 were investigated. Results indicated that cell growth was maximal at pH 5.0, while pH 4.5 was preferable to GABA formation. The optimal temperature for cell growth (35 ℃) was lower than that for GABA formation (40 ℃). In a two-stage pH and temperature control fermentation, cultures were maintained at pH 5.0 and 35 ℃ for 32 h, then adjusted to pH 4.5 and 40 ℃, GABA production increased remarkably and reached 474.79 mmol·L^-1 at 72 h, while it was 398.63 mmol·L^-1 with one stage pH and temperature control process, in which cultivation conditions were constantly controlled at pH 5.0 and 35 ℃. In order to avoid the inhibition of cell growth at higher L-monosodium glutamate (L-MSG) concentrations, the two-stage control fermentation with substrate feeding strategy was applied to GABA production, with 106.87 mmol (20 g) L-MSG supplemented into the shaking-flask at 32 h and 56 h post-inoculation separately. The GABA concentration reached 526.33 mmol·L^-1 at 72 h with the fermentation volume increased by 38%. These results will provide primary data to realize large-scale production of GABA by L. brevis CGMCC 1306.

Key words: gamma-aminobutyric acid, Lactobacillus brevis CGMCC 1306, two-stage pH and temperature control, substrate feeding

彭春龙, 黄俊, 胡升, 赵伟睿, 姚善泾, 梅乐和. A Two-stage pH and Temperature Control with Substrate Feeding Strategy for Production of Gamma-aminobutyric Acid by Lactobacillus brevis CGMCC 1306[J]. Chinese Journal of Chemical Engineering, 2013, 21(10): 1190-1194.

PENG Chunlong, HUANG Jun, HU Sheng, ZHAO Weirui, YAO Shanjing, MEI Lehe. A Two-stage pH and Temperature Control with Substrate Feeding Strategy for Production of Gamma-aminobutyric Acid by Lactobacillus brevis CGMCC 1306[J]. Chin.J.Chem.Eng., 2013, 21(10): 1190-1194.

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A Two-stage pH and Temperature Control with Substrate Feeding Strategy for Production of Gamma-aminobutyric Acid by Lactobacillus brevis CGMCC 1306

A Two-stage pH and Temperature Control with Substrate Feeding Strategy for Production of Gamma-aminobutyric Acid by Lactobacillus brevis CGMCC 1306

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