Novel Gas-assisted Three-liquid-phase Extraction System for Simultaneous Separation and Concentration of Anthraquinones in Herbal Extract

doi:10.1016/j.cjche.2014.06.029

›› 2014, Vol. 22 ›› Issue (9): 968-973.DOI: 10.1016/j.cjche.2014.06.029

Novel Gas-assisted Three-liquid-phase Extraction System for Simultaneous Separation and Concentration of Anthraquinones in Herbal Extract

Xingfu Yang^1,2, Xiangfeng Liang¹, Liangrong Yang¹, Feng Pan¹, Fuli Deng^1,2, Huizhou Liu^1,3

1 Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

收稿日期:2013-03-25 修回日期:2013-07-10 出版日期:2014-09-28 发布日期:2014-11-04
通讯作者: Xiangfeng Liang, Huizhou Liu
基金资助:
Supported by the National Natural Science Foundation of China (21136009, 21106162, 21106152), Ministry of Human Resources and Social Security of China and State Key Laboratory of Chemical Engineering (SKL-ChE-11A04).

Novel Gas-assisted Three-liquid-phase Extraction System for Simultaneous Separation and Concentration of Anthraquinones in Herbal Extract

Xingfu Yang^1,2, Xiangfeng Liang¹, Liangrong Yang¹, Feng Pan¹, Fuli Deng^1,2, Huizhou Liu^1,3

1 Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

Received:2013-03-25 Revised:2013-07-10 Online:2014-09-28 Published:2014-11-04
Supported by:
Supported by the National Natural Science Foundation of China (21136009, 21106162, 21106152), Ministry of Human Resources and Social Security of China and State Key Laboratory of Chemical Engineering (SKL-ChE-11A04).

摘要/Abstract

摘要： Gas-assisted three-liquid-phase extraction (GATE), which has the advantages of both three-liquid-phase extraction and solvent sublation, is a novel separation technique for separation and concentration of two organic compounds into different phases in one step. This highly effective and economically applicable method has been developed for separating emodin and rhein from herbal extract. In a GATE system composed of butyl acetate/ PEG4000/ammoniumsulfate aqueous solution, influence of various parameters including gas flow rate, flotation time, salt concentration, initial volumeof PEG and butyl acetatewas investigated. Within 50 min of 30 ml·min^-1 nitrogen flow, removal ratio of emodin and rhein from aqueous phase could be over 99% and 97%, respectively. Mass fraction of emodin in the BA phase and rhein in the PEG phase could reach 97% and 95%, respectively. It is demonstrated that gas bubbling is effective for partitioning of emodin and rhein into butyl acetate and PEG phase respectively, and dispersed PEG and butyl acetate could be captured from the aqueous solution. Experimental results showthat GATE could be an effective and economical technology for concentration and separation of co-existed products in medicinal plants.

关键词: Three-liquid-phase extraction, Gas-assisted solvent extraction, Separation, Anthraquinones

Abstract: Gas-assisted three-liquid-phase extraction (GATE), which has the advantages of both three-liquid-phase extraction and solvent sublation, is a novel separation technique for separation and concentration of two organic compounds into different phases in one step. This highly effective and economically applicable method has been developed for separating emodin and rhein from herbal extract. In a GATE system composed of butyl acetate/ PEG4000/ammoniumsulfate aqueous solution, influence of various parameters including gas flow rate, flotation time, salt concentration, initial volumeof PEG and butyl acetatewas investigated. Within 50 min of 30 ml·min^-1 nitrogen flow, removal ratio of emodin and rhein from aqueous phase could be over 99% and 97%, respectively. Mass fraction of emodin in the BA phase and rhein in the PEG phase could reach 97% and 95%, respectively. It is demonstrated that gas bubbling is effective for partitioning of emodin and rhein into butyl acetate and PEG phase respectively, and dispersed PEG and butyl acetate could be captured from the aqueous solution. Experimental results showthat GATE could be an effective and economical technology for concentration and separation of co-existed products in medicinal plants.

Key words: Three-liquid-phase extraction, Gas-assisted solvent extraction, Separation, Anthraquinones

Xingfu Yang, Xiangfeng Liang, Liangrong Yang, Feng Pan, Fuli Deng, Huizhou Liu. Novel Gas-assisted Three-liquid-phase Extraction System for Simultaneous Separation and Concentration of Anthraquinones in Herbal Extract[J]. , 2014, 22(9): 968-973.

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Novel Gas-assisted Three-liquid-phase Extraction System for Simultaneous Separation and Concentration of Anthraquinones in Herbal Extract

Novel Gas-assisted Three-liquid-phase Extraction System for Simultaneous Separation and Concentration of Anthraquinones in Herbal Extract

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