Enhanced production of glycyrrhetic acid 3-O-mono-β-D-glucuronide by fed-batch fermentation using pH and dissolved oxygen as feedback parameters

doi:10.1016/j.cjche.2015.12.003

Chinese Journal of Chemical Engineering ›› 2016, Vol. 24 ›› Issue (4): 506-512.DOI: 10.1016/j.cjche.2015.12.003

• 第25届中国过程控制会议专栏 • 上一篇下一篇

Enhanced production of glycyrrhetic acid 3-O-mono-β-D-glucuronide by fed-batch fermentation using pH and dissolved oxygen as feedback parameters

Bo Lü, Xiaogang Yang, Xudong Feng, Chun Li

School of Life Science, Beijing Institute of Technology, Beijing 100081, China

收稿日期:2015-05-06 修回日期:2015-07-16 出版日期:2016-04-28 发布日期:2016-05-27
通讯作者: Xudong Feng
基金资助:
Supported by the National Natural Science Foundation of China (21176028 and 21506011), the National Science Fund for Distinguished Young Scholars of China (21425624) and Doctoral Fund of Ministry of Education of China (20121101110050).

Enhanced production of glycyrrhetic acid 3-O-mono-β-D-glucuronide by fed-batch fermentation using pH and dissolved oxygen as feedback parameters

Bo Lü, Xiaogang Yang, Xudong Feng, Chun Li

School of Life Science, Beijing Institute of Technology, Beijing 100081, China

Received:2015-05-06 Revised:2015-07-16 Online:2016-04-28 Published:2016-05-27
Contact: Xudong Feng
Supported by:
Supported by the National Natural Science Foundation of China (21176028 and 21506011), the National Science Fund for Distinguished Young Scholars of China (21425624) and Doctoral Fund of Ministry of Education of China (20121101110050).

摘要/Abstract

摘要： Glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG), the major functional ingredient in licorice, has widespread applications in food, pharmacy and cosmetics industry. The production of GAMG through Penicillium purpurogenum Li-3 cultivationwas for the first time performed through both batch and fed-batch processes in bioreactors. In batch process, under optimal conditions (pH 5.0, temperature 32℃, agitation speed 100 r·min^-1), 3.55 g·L^-1 GAMGwas obtained in a 2.5 L fermentor. To further enhance GAMG production, a fine fed-batch process was developed by using pH and DO as feedback parameters. Starting from 48 h, 100 ml 90 g·L^-1 substrate Glycyrrhizin (GL) was fed each time when pH increased to above 5.0 and DO was increased to above 80%. This strategy can significantly enhance GAMG production: the achieved GL conversion was 95.34% with GAMG yield of 95.15%, and GAMG concentration was 16.62 g·L^-1 which was 5 times higher than that of batch. Then, a two-step separation strategy was established to separate GAMG from fermentation broth by crude extraction of 15 ml column packed with D101 resin followed by fine purification with preparative C18 chromatography. The obtainedGAMG purity was 95.79%. This study provides a new insight into the industrial bioprocess of high-level GAMG production.

关键词: Glycyrrhetic acid 3-O-mono-&, beta, -D-glucuronide, Fed-batch fermentation, Penicillium purpurogenum Li-3, D101 resin separation

Abstract: Glycyrrhetic acid 3-O-mono-β-D-glucuronide (GAMG), the major functional ingredient in licorice, has widespread applications in food, pharmacy and cosmetics industry. The production of GAMG through Penicillium purpurogenum Li-3 cultivationwas for the first time performed through both batch and fed-batch processes in bioreactors. In batch process, under optimal conditions (pH 5.0, temperature 32℃, agitation speed 100 r·min^-1), 3.55 g·L^-1 GAMGwas obtained in a 2.5 L fermentor. To further enhance GAMG production, a fine fed-batch process was developed by using pH and DO as feedback parameters. Starting from 48 h, 100 ml 90 g·L^-1 substrate Glycyrrhizin (GL) was fed each time when pH increased to above 5.0 and DO was increased to above 80%. This strategy can significantly enhance GAMG production: the achieved GL conversion was 95.34% with GAMG yield of 95.15%, and GAMG concentration was 16.62 g·L^-1 which was 5 times higher than that of batch. Then, a two-step separation strategy was established to separate GAMG from fermentation broth by crude extraction of 15 ml column packed with D101 resin followed by fine purification with preparative C18 chromatography. The obtainedGAMG purity was 95.79%. This study provides a new insight into the industrial bioprocess of high-level GAMG production.

Key words: Glycyrrhetic acid 3-O-mono-&, beta, -D-glucuronide, Fed-batch fermentation, Penicillium purpurogenum Li-3, D101 resin separation

Bo Lü, Xiaogang Yang, Xudong Feng, Chun Li. Enhanced production of glycyrrhetic acid 3-O-mono-β-D-glucuronide by fed-batch fermentation using pH and dissolved oxygen as feedback parameters[J]. Chinese Journal of Chemical Engineering, 2016, 24(4): 506-512.

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Enhanced production of glycyrrhetic acid 3-O-mono-β-D-glucuronide by fed-batch fermentation using pH and dissolved oxygen as feedback parameters

Enhanced production of glycyrrhetic acid 3-O-mono-β-D-glucuronide by fed-batch fermentation using pH and dissolved oxygen as feedback parameters

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