Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride

›› 2010, Vol. 18 ›› Issue (4): 533-537.

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Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride

吴丹, 陈灏, 江凌, 蔡谨, 徐志南, 岑沛霖

Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

收稿日期:2009-12-15 修回日期:2010-06-06 出版日期:2010-08-28 发布日期:2010-08-28
通讯作者: XU Zhinan, E-mail:znxu@zju.edu.cn
基金资助:
Supported by the National High Technology Research and Development Program of China(2009AA02Z206,2006AA02Z239);the National Basic Research Program of China(2007CB707805);the Ministry of Science and Technology,China

Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride

WU Dan, CHEN Hao, JIANG Ling, CAI Jin, XU Zhinan, CEN Peilin

Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

Received:2009-12-15 Revised:2010-06-06 Online:2010-08-28 Published:2010-08-28

摘要/Abstract

摘要： One of the bottlenecks for bioproduction of butyric acid as bulk chemical is the difficulty in separating butyric acid from the fermentation broth,compared with the petroleum-based chemical synthesis method.In the present work,a novel separation methodology was developed based on an aqueous two-phase system with inor-ganic salts.Calcium chloride was screened out for effective separation of butyric acid from butyric acid-water-salt systems.Within appropriate concentration range of butyric acid and salt,butyric acid was enriched in the upper phase and most of calcium ions remained in the lower phase.This"salting out"effect is very efficient to separate butyric acid from the simulated butyrate fermentation broth,which consists of butyric acid and acetic acid with concentration ratio of 4:1,so that the final ratio of butyric acid/acetic acid in the upper phase is improved to 9.87. The aqueous two-phase system was used to separate butyric acid from the actual fermentation broth with satisfactory result.

关键词: butyric acid, separation, aqueous two-phase partition, salting out effect, calcium chloride

Abstract: One of the bottlenecks for bioproduction of butyric acid as bulk chemical is the difficulty in separating butyric acid from the fermentation broth,compared with the petroleum-based chemical synthesis method.In the present work,a novel separation methodology was developed based on an aqueous two-phase system with inor-ganic salts.Calcium chloride was screened out for effective separation of butyric acid from butyric acid-water-salt systems.Within appropriate concentration range of butyric acid and salt,butyric acid was enriched in the upper phase and most of calcium ions remained in the lower phase.This"salting out"effect is very efficient to separate butyric acid from the simulated butyrate fermentation broth,which consists of butyric acid and acetic acid with concentration ratio of 4:1,so that the final ratio of butyric acid/acetic acid in the upper phase is improved to 9.87. The aqueous two-phase system was used to separate butyric acid from the actual fermentation broth with satisfactory result.

Key words: butyric acid, separation, aqueous two-phase partition, salting out effect, calcium chloride

吴丹, 陈灏, 江凌, 蔡谨, 徐志南, 岑沛霖. Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride[J]. , 2010, 18(4): 533-537.

WU Dan, CHEN Hao, JIANG Ling, CAI Jin, XU Zhinan, CEN Peilin. Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride[J]. , 2010, 18(4): 533-537.

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Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride

Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride

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