Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid

›› 2011, Vol. 19 ›› Issue (4): 636-643.

Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid

陈秉梅¹, 许小平¹, 侯志国¹, 李忠琴², 阮文兵¹

1. College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China;
2. College of Fisheries, Jimei University, Xiamen 361021, China

收稿日期:2011-01-06 修回日期:2011-04-18 出版日期:2011-08-28 发布日期:2011-08-28
通讯作者: XU Xiaoping,E-mail:xu@fzu.edu.cn
基金资助:
Supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (20103514110002);the Science and Technology Key Project of Fujian Province (2009N0046)

Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid

CHEN Bingmei¹, XU Xiaoping¹, HOU Zhiguo¹, LI Zhongqin², RUAN Wenbing¹

1. College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China;
2. College of Fisheries, Jimei University, Xiamen 361021, China

Received:2011-01-06 Revised:2011-04-18 Online:2011-08-28 Published:2011-08-28
Supported by:
Supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (20103514110002);the Science and Technology Key Project of Fujian Province (2009N0046)

摘要/Abstract

摘要： A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield, 47.5%;enantiomeric excess, 99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacillus sp.B26 by 16S rDNA(ribosomal DNA) sequencing.Phylogenic analysis showed that the strain was most similar to Bacillus sp.enrichment culture clone SYW5(FJ601635.1) and Bacillus cereus strain FM-4(EU794727.1).Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis.Among viable mutants, B.sp.UV-38 and B.sp.ULi-11 exhibited better productivities than the wild type.Comparisons of HPA production and time course among wild strain and two mutants showed that B.sp.ULi-11 was more competent than B.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11(yield, 65.4%) compared to that with B.sp.B26(yield, 47.0%) when cultured in fermentation broth(pH 7.2) at 32℃ with an agitation speed of 180 r·min^-1 and PGA final concentration of 15 mmol·L^-1 for 25 h.

关键词: (R)-α-hydroxyphenylacetic acid, biotransformation, phylogenetic analysis, Bacillus, phenylglyoxylic acid, UV mutagenesis

Abstract: A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield, 47.5%;enantiomeric excess, 99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacillus sp.B26 by 16S rDNA(ribosomal DNA) sequencing.Phylogenic analysis showed that the strain was most similar to Bacillus sp.enrichment culture clone SYW5(FJ601635.1) and Bacillus cereus strain FM-4(EU794727.1).Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis.Among viable mutants, B.sp.UV-38 and B.sp.ULi-11 exhibited better productivities than the wild type.Comparisons of HPA production and time course among wild strain and two mutants showed that B.sp.ULi-11 was more competent than B.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11(yield, 65.4%) compared to that with B.sp.B26(yield, 47.0%) when cultured in fermentation broth(pH 7.2) at 32℃ with an agitation speed of 180 r·min^-1 and PGA final concentration of 15 mmol·L^-1 for 25 h.

Key words: (R)-α-hydroxyphenylacetic acid, biotransformation, phylogenetic analysis, Bacillus, phenylglyoxylic acid, UV mutagenesis

陈秉梅, 许小平, 侯志国, 李忠琴, 阮文兵. Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid [J]. , 2011, 19(4): 636-643.

CHEN Bingmei, XU Xiaoping, HOU Zhiguo, LI Zhongqin, RUAN Wenbing. Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid [J]. , 2011, 19(4): 636-643.

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Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid

Identification and Mutagenesis of a New Isolated Strain Bacillus sp. B26 for Producing(R)-α-Hydroxyphenylacetic Acid

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