重组大肠杆菌可溶表达并快速定量GFP-hepA融合蛋白

• BIOTECHNOLOGY & BIOENGINEERING • 上一篇下一篇

重组大肠杆菌可溶表达并快速定量GFP-hepA融合蛋白

陈银; 邢新会; 叶逢春; 况莹

Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-28 发布日期:2007-02-28
通讯作者: 陈银

Soluble expression and rapid quantification of GFP-hepA fusion protein in recombinant Escherichia coli

CHEN Yin; XING Xinhui; YE Fengchun; KUANG Ying

Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-28 Published:2007-02-28
Contact: CHEN Yin

摘要/Abstract

摘要： To establish a rapid quantification method for heparinase I during its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase I to the C terminus of a green fluorescent protein mutant (GFPmut1). As a result, not only was the functional recombinant expression of heparinase I in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase I activity, allowing enzyme activity to be quantified rapidly during the fermentation.

关键词: functional expression;fusion protein;green fluorescent protein (GFP);heparinase I;rapid quantification

Abstract: To establish a rapid quantification method for heparinase I during its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase I to the C terminus of a green fluorescent protein mutant (GFPmut1). As a result, not only was the functional recombinant expression of heparinase I in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase I activity, allowing enzyme activity to be quantified rapidly during the fermentation.

Key words: functional expression, fusion protein, green fluorescent protein (GFP), heparinase I, rapid quantification

陈银, 邢新会, 叶逢春, 况莹. 重组大肠杆菌可溶表达并快速定量GFP-hepA融合蛋白[J]. , DOI: .

CHEN ,Yin, XING ,Xinhui, YE ,Fengchun, KUANG ,Ying. Soluble expression and rapid quantification of GFP-hepA fusion protein in recombinant Escherichia coli[J]. , DOI: .

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