Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

doi:10.1016/j.cjche.2024.01.008

Chinese Journal of Chemical Engineering ›› 2024, Vol. 69 ›› Issue (5): 56-62.DOI: 10.1016/j.cjche.2024.01.008

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Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

Kaixin Han¹, Yibo Zeng², Yinghua Lu¹, Ping Zeng³, Liang Shen¹

1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
2. Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen 361005, China;
3. Chinese Research Academy of Environmental Sciences, Beijing 100012, China

Received:2023-09-26 Revised:2023-12-30 Online:2024-07-01 Published:2024-05-28
Contact: Ping Zeng,E-mail:zengping@craes.org.cn;Liang Shen,E-mail:shenliang@xmu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (22178293) and the Natural Science Foundation of Fujian Province of China (2022J01022).

Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

Kaixin Han¹, Yibo Zeng², Yinghua Lu¹, Ping Zeng³, Liang Shen¹

1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
2. Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen 361005, China;
3. Chinese Research Academy of Environmental Sciences, Beijing 100012, China

通讯作者: Ping Zeng,E-mail:zengping@craes.org.cn;Liang Shen,E-mail:shenliang@xmu.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (22178293) and the Natural Science Foundation of Fujian Province of China (2022J01022).

Abstract

Abstract: The bioreduction of graphene oxide (GO) using environmentally functional bacteria such as Shewanella represents a green approach to produce reduced graphene oxide (rGO). This process differs from the chemical reduction that involves instantaneous molecular reactions. In bioreduction, the contact of bacterial cells and GO is considered the rate-limiting step. To reveal how the bacteria-GO integration regulates rGO production, the comparative experiments of GO and three Shewanella strains were carried out. Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and atomic force microscopy were used to characterize the reduction degree and the aggregation degree. The results showed that a spontaneous aggregation of GO and Shewanella into the condensed entity occurred within 36 h. A positive linear correlation was established, linking three indexes of the aggregation potential, the bacterial reduction ability, and the reduction degree (I_D/I_G) comprehensively.

Key words: Graphene oxide, Reduced graphene oxide, Bioreduction, Aggregation, Shewanella

摘要： The bioreduction of graphene oxide (GO) using environmentally functional bacteria such as Shewanella represents a green approach to produce reduced graphene oxide (rGO). This process differs from the chemical reduction that involves instantaneous molecular reactions. In bioreduction, the contact of bacterial cells and GO is considered the rate-limiting step. To reveal how the bacteria-GO integration regulates rGO production, the comparative experiments of GO and three Shewanella strains were carried out. Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and atomic force microscopy were used to characterize the reduction degree and the aggregation degree. The results showed that a spontaneous aggregation of GO and Shewanella into the condensed entity occurred within 36 h. A positive linear correlation was established, linking three indexes of the aggregation potential, the bacterial reduction ability, and the reduction degree (I_D/I_G) comprehensively.

关键词: Graphene oxide, Reduced graphene oxide, Bioreduction, Aggregation, Shewanella

Kaixin Han, Yibo Zeng, Yinghua Lu, Ping Zeng, Liang Shen. Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria[J]. Chinese Journal of Chemical Engineering, 2024, 69(5): 56-62.

Kaixin Han, Yibo Zeng, Yinghua Lu, Ping Zeng, Liang Shen. Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria[J]. 中国化学工程学报, 2024, 69(5): 56-62.

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Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

Aggregation-regulated bioreduction process of graphene oxide by Shewanella bacteria

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