Interaction of DNA with Cationic Gemini Surfactant Trimethylene-1, 3-bis(dodecyldimethyl-ammonium bromide) and Anionic Surfactant SDS Mixed System

Abstract

Abstract: The interaction of DNA with cationic gemini surfactant trimethylene-1,3-bis(dodecyl dimethyl-ammonium bromide)(12-3-12)and anionic surfactant sodium dodecyl sulfate(SDS) mixed system has been investigated by measuring the fluorescence,zeta potential,UV-V is spectrum,and circular dichroism.In the absence of SDS,owing to the electrostatic and hydrophobic interactions,12-3-12 forms micelle-like structure on the DNA chain before the micellization in bulk phase.For the mixed system of 12-3-12 and SDS,the negative charges on SDS can compete against DNA to bind with cationic 12-3-12 because of the stronger interaction between oppositely charged surfactants,and thus,the catanionic mixed micelles are formed before the formation of DNA/12-3-12 complexes.Thereafter,the positive charges on the mixed micelles bind with DNA,and thus,the change of the zeta potential from negative to positive is distinctly different from the system without SDS.Meanwhile,the existence of SDS postpones the exclusion of ethidium bromide(EB) from DNA/EB complexes.The conformation of DNA undergoes a change from native B-form to chiralψ-phase as binding with 12-3-12 process.Upon adding SDS to the DNA/12-3-12 complex solution,however,DNA is released to the bulk and theψ-phase returns to B-form again.

Key words: interaction, DNA, gemini surfactant, SDS, conformation

摘要： The interaction of DNA with cationic gemini surfactant trimethylene-1,3-bis(dodecyl dimethyl-ammonium bromide)(12-3-12)and anionic surfactant sodium dodecyl sulfate(SDS) mixed system has been investigated by measuring the fluorescence,zeta potential,UV-V is spectrum,and circular dichroism.In the absence of SDS,owing to the electrostatic and hydrophobic interactions,12-3-12 forms micelle-like structure on the DNA chain before the micellization in bulk phase.For the mixed system of 12-3-12 and SDS,the negative charges on SDS can compete against DNA to bind with cationic 12-3-12 because of the stronger interaction between oppositely charged surfactants,and thus,the catanionic mixed micelles are formed before the formation of DNA/12-3-12 complexes.Thereafter,the positive charges on the mixed micelles bind with DNA,and thus,the change of the zeta potential from negative to positive is distinctly different from the system without SDS.Meanwhile,the existence of SDS postpones the exclusion of ethidium bromide(EB) from DNA/EB complexes.The conformation of DNA undergoes a change from native B-form to chiralψ-phase as binding with 12-3-12 process.Upon adding SDS to the DNA/12-3-12 complex solution,however,DNA is released to the bulk and theψ-phase returns to B-form again.

关键词: interaction, DNA, gemini surfactant, SDS, conformation

ZHAO Xiaofang, SHANG Yazhuo, LIU Honglai, HU Ying, JIANG Jianwen. Interaction of DNA with Cationic Gemini Surfactant Trimethylene-1, 3-bis(dodecyldimethyl-ammonium bromide) and Anionic Surfactant SDS Mixed System[J]. , 2008, 16(6): 923-928.

赵小芳, 尚亚卓, 刘洪来, 胡英, 姜建文. Interaction of DNA with Cationic Gemini Surfactant Trimethylene-1, 3-bis(dodecyldimethyl-ammonium bromide) and Anionic Surfactant SDS Mixed System[J]. , 2008, 16(6): 923-928.

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