SCI和EI收录∣中国化工学会会刊

›› 2010, Vol. 18 ›› Issue (6): 1013-1017.

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles     Next Articles

Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

LIU Jiefeng1,2, REN Yiran1, YAO Shanjing1   

  1. 1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
    2. Department of Biotechnology and Food Engineering, Maoming College, Maoming 525000, China
  • Received:2010-04-29 Revised:2010-07-16 Online:2010-12-28 Published:2010-12-28
  • Supported by:
    Supported by the National Basic Research Program of China(2007CB707805);the National Natural Science Foundation of China(20876139)

Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production

刘杰凤1,2, 任意然1, 姚善泾1   

  1. 1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
    2. Department of Biotechnology and Food Engineering, Maoming College, Maoming 525000, China
  • 通讯作者: YAO Shanjing, E-mail: yaosj@zju.edu.cn
  • 基金资助:
    Supported by the National Basic Research Program of China(2007CB707805);the National Natural Science Foundation of China(20876139)

Abstract: In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were prepared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule’s membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500),which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.

Key words: microencapsulation, Monascus purpureus, natural pigment, polyelectrolyte complex, NaCS-PDMDAAC capsule, cell immobilization

摘要: In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were prepared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule’s membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500),which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture.

关键词: microencapsulation, Monascus purpureus, natural pigment, polyelectrolyte complex, NaCS-PDMDAAC capsule, cell immobilization