Construction and evaluation of co-electrospun poly (butylene succinate)/gelatin materials as potential vascular grafts

doi:10.1016/j.cjche.2021.06.015

Abstract

Abstract: In this study, a series of poly (butylene succinate) (PBSU)/gelatin composites were prepared by electrospinning. The morphology, physicochemical analysis, biomechanical properties, biocompatibility, and biodegradability of the materials were evaluated. The results showed that the ultimate tensile stress of the vascular PBSU/gelatin grafts at (95/5), (90/10), (85/15), and (80/20) was (4.17±0.54) MPa, (3.81±0.44) MPa, 2.94±0.69 MPa and 2.11±0.72 MPa respectively, and the burst pressure was (282.7±22.3) kPa, (295.3±3.9) kPa, (306.8±13.9) kPa and (307.6±9.0) kPa respectively, which met the requirements of tissue-engineered blood vessels. Furthermore, the addition of gelatin improved the hydrophilicity and degradation properties of PBSU, thus enhancing cell adhesion and promoting the inward growth of vascular smooth muscle cells. In summary, the research in this paper provides a useful reference for the preparation and optimization of vascular scaffolds.

Key words: Poly (butylene succinate), Gelatin, Co-electrospinning, Degradation, Vascular graft

摘要： In this study, a series of poly (butylene succinate) (PBSU)/gelatin composites were prepared by electrospinning. The morphology, physicochemical analysis, biomechanical properties, biocompatibility, and biodegradability of the materials were evaluated. The results showed that the ultimate tensile stress of the vascular PBSU/gelatin grafts at (95/5), (90/10), (85/15), and (80/20) was (4.17±0.54) MPa, (3.81±0.44) MPa, 2.94±0.69 MPa and 2.11±0.72 MPa respectively, and the burst pressure was (282.7±22.3) kPa, (295.3±3.9) kPa, (306.8±13.9) kPa and (307.6±9.0) kPa respectively, which met the requirements of tissue-engineered blood vessels. Furthermore, the addition of gelatin improved the hydrophilicity and degradation properties of PBSU, thus enhancing cell adhesion and promoting the inward growth of vascular smooth muscle cells. In summary, the research in this paper provides a useful reference for the preparation and optimization of vascular scaffolds.

关键词: Poly (butylene succinate), Gelatin, Co-electrospinning, Degradation, Vascular graft

Tingting Yang, Han Xu, Congrui Zhao, Di Tang, Fan Mu, Hongjiang Lu, Zhoufeng Rao, Shufang Wang. Construction and evaluation of co-electrospun poly (butylene succinate)/gelatin materials as potential vascular grafts[J]. Chinese Journal of Chemical Engineering, 2021, 39(11): 297-305.

References

[1] H.-H. Song, R.T. Rumma, C.K. Ozaki, E.R. Edelman, C.S. Chen, Vascular tissue engineering:progress, challenges, and clinical promise, Cell Stem Cell. 22(3) (2018) 340-354.
[2] J. Luo, L. Qin, L. Zhao, L. Gui, M.W. Ellis, Y. Huang, M.H. Kural, J.A. Clark, S. Ono, J. Wang, Y. Yuan, S.M. Zhang, X. Cong, G. Li, M. Riaz, C. Lopez, A. Hotta, S. Campbell, G. Tellides, A. Dardik, L.E. Niklason, Y. Qyang, Tissue-engineered vascular grafts with advanced mechanical strength from human iPSCs, Cell Stem Cell. 26(2) (2020) 251-261, e8.
[3] L.E. Niklason, J.H. Lawson, Bioengineered human blood vessels, Science 370(6513) (2020) eaaw8682.
[4] S. Nazarnezhad, F. Baino, H.W. Kim, T.J. Webster, S. Kargozar, Electrospun nanofibers for improved angiogenesis:promises for tissue engineering applications, Nanomaterials (Basel) 10(8) (2020) E1609.
[5] A. Kruger-Genge, A. Blocki, R.P. Franke, F. Jung, Vascular endothelial cell biology:an update, Int. J. Mol. Sci. 20(18) (2019) E4411.
[6] L. Andrique, G. Recher, K. Alessandri, N. Pujol, M. Feyeux, P. Bon, L. Cognet, P. Nassoy, A. Bikfalvi, A model of guided cell self-organization for rapid and spontaneous formation of functional vessels, Sci. Adv. 5(6) (2019) eaau6562.
[7] A.A. Marek, V. Verney, C. Taviot-Gueho, G. Totaro, L. Sisti, A. Celli, F. Leroux, Outstanding chain-extension effect and high UV resistance of polybutylene succinate containing amino-acid-modified layered double hydroxides, Beilstein. J. Nanotechnol. 10(2019) 684-695.
[8] M. Ojansivu, L. Johansson, S. Vanhatupa, I. Tamminen, M. Hannula, J. Hyttinen, M. Kellomäki, S. Miettinen, Knitted 3D Scaffolds of polybutylene succinate support human mesenchymal stem cell growth and osteogenesis, Stem Cells Int. 2018(2018) 1-11.
[9] V.P. Ribeiro, L.R. Almeida, A.R. Martins, I. Pashkuleva, A.P. Marques, A.S. Ribeiro, C.J. Silva, G. Bonifácio, R.A. Sousa, A.L. Oliveira, R.L. Reis, Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications, J. Tissue Eng. Regen. Med. 11(10) (2017) 2853-2863.
[10] K.e. Shi, Y. Liu, X. Hu, T. Su, P. Li, Z. Wang, Preparation, characterization, and biodegradation of poly(butylene succinate)/cellulose triacetate blends, Int. J. Biol. Macromol. 114(2018) 373-380.
[11] W. Zhang, Y. Xiang, H. Fan, L. Wang, Y. Xie, G. Zhao, Y. Liu, Biodegradable ureaformaldehyde/PBS and its ternary nanocomposite prepared by a novel and scalable reactive extrusion process for slow-release applications in agriculture, J. Agric. Food Chem. 68(16) (2020) 4595-4606.
[12] X. Zhou, T. Wu, Synthesis, characterization of phosphorus-containing copolyester and its application as flame retardants for poly(butylene succinate) (PBS), Chemosphere 235(2019) 163-168.
[13] A.R. de Matos Costa, A. Crocitti, L. Hecker de Carvalho, S.C. Carroccio, P. Cerruti, G. Santagata, Properties of biodegradable films based on poly(butylene succinate) (PBS) and poly(butylene adipate-co-terephthalate) (PBAT) blends (dagger, double dagger), Polymers (Basel) 12(10) (2020) 2317.
[14] S. Freeman, R. Ramos, P. Alexis Chando, L. Zhou, K. Reeser, S. Jin, P. Soman, K. Ye, A bioink blend for rotary 3D bioprinting tissue engineered small-diameter vascular constructs, Acta Biomater. 95(2019) 152-164.
[15] M.C. Echave, R. Hernáez-Moya, L. Iturriaga, J.L. Pedraz, R. Lakshminarayanan, A. Dolatshahi-Pirouz, N. Taebnia, G. Orive, Recent advances in gelatin-based therapeutics, Expert Opin. Biol. Ther. 19(8) (2019) 773-779.
[16] O. Madkhali, G. Mekhail, S.D. Wettig, Modified gelatin nanoparticles for gene delivery, Int. J. Pharm. 554(2019) 224-234.
[17] H.D. López-Calderón, H. Avilés-Arnaut, L.J. Galán-Wong, V. Almaguer-Cantú, J. R. Laguna-Camacho, C. Calderón-Ramón, J.E. Escalante-Martínez, K. ArévaloNiño, Electrospun polyvinylpyrrolidone-gelatin and cellulose acetate bi-layer scaffold loaded with gentamicin as possible wound dressing, Polymers (Basel) 12(10) (2020) 2311.
[18] A. Gaspar-Pintiliescu, A.M. Stanciuc, O. Craciunescu, Natural composite dressings based on collagen, gelatin and plant bioactive compounds for wound healing:A review, Int. J. Biol. Macromol. 138(2019) 854-865.
[19] Y. Liang, B. Chen, M. Li, J. He, Z. Yin, B. Guo, Injectable antimicrobial conductive hydrogels for wound disinfection and infectious wound healing, Biomacromolecules 21(5) (2020) 1841-1852.
[20] F. Tondnevis, H. Keshvari, J.A. Mohandesi, Fabrication, characterization, and in vitro evaluation of electrospun polyurethane-gelatin-carbon nanotube scaffolds for cardiovascular tissue engineering applications, J. Biomed. Mater. Res. B Appl. Biomater. 108(5) (2020) 2276-2293.
[21] H.-H. Cheng, J. Xiong, Z.-N. Xie, Y.-T. Zhu, Y.-M. Liu, Z.-Y. Wu, J. Yu, Z.-X. Guo, Thrombin-loaded poly(butylene succinate)-based electrospun membranes for rapid hemostatic application, Macromol. Mater. Eng. 303(2) (2018) 1700395.
[22] A. Yin, K. Zhang, M.J. McClure, C. Huang, J. Wu, J. Fang, X. Mo, G.L. Bowlin, S.S. Al-Deyab, M. El-Newehy, Electrospinning collagen/chitosan/poly(L-lactic acidco-epsilon-caprolactone) to form a vascular graft:Mechanical and biological characterization, J. Biomed. Mater. Res. A 101(5) (2013) 1292-1301.
[23] C. Xu, R. Inai, M. Kotaki, S. Ramakrishna, Electrospun nanofiber fabrication as synthetic extracellular matrix and its potential for vascular tissue engineering, Tissue Eng. 10(7-8) (2004) 1160-1168.
[24] S. Laurent, X. Girerd, J.J. Mourad, P. Lacolley, L. Beck, P. Boutouyrie, J.P. Mignot, M. Safar, Elastic modulus of the radial artery wall material is not increased in patients with essential hypertension, Arterioscler. Thromb. 14(7) (1994) 1223-1231.
[25] F. Montini-Ballarin, D. Calvo, P.C. Caracciolo, F. Rojo, P.M. Frontini, G.A. Abraham, V.G. G, Mechanical behavior of bilayered small-diameter nanofibrous structures as biomimetic vascular grafts, J. Mech. Behav. Biomed. Mater. 60(2016) 220-233.
[26] X. Li, H. Zhao, Mechanical and degradation properties of small-diameter vascular grafts in an in vitro biomimetic environment, J. Biomater. Appl. 33(8) (2019) 1017-1034.
[27] H. Cui, W. Zhu, Y. Huang, C. Liu, Z.X. Yu, M. Nowicki, S. Miao, Y. Cheng, X. Zhou, S.J. Lee, Y. Zhou, S. Wang, M. Mohiuddin, K. Horvath, L.G. Zhang, In vitro and in vivo evaluation of 3D bioprinted small-diameter vasculature with smooth muscle and endothelium, Biofabrication 12(1) (2019) 015004.
[28] L. Shao, Q. Gao, H. Zhao, C. Xie, J. Fu, Z. Liu, M. Xiang, Y. He, Fiber-based mini tissue with morphology-controllable GelMA microfibers, Small 14(44) (2018) e1802187.