Polymeric assembled nanoparticles through kinetic stabilization by confined impingement jets dilution mixer for fluorescence switching imaging

doi:10.1016/j.cjche.2022.06.034

Chinese Journal of Chemical Engineering ›› 2023, Vol. 56 ›› Issue (4): 89-96.DOI: 10.1016/j.cjche.2022.06.034

Polymeric assembled nanoparticles through kinetic stabilization by confined impingement jets dilution mixer for fluorescence switching imaging

Jingran Liu¹, Yue Wu¹, Jie Tang¹, Tao Wang¹, Feng Ni², Qiumin Wu¹, Xijiao Yang¹, Ayyaz Ahmad³, Naveed Ramzan⁴, Yisheng Xu¹

1. State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China;
2. Shanghai Shyndec Pharmaceutical Co., Ltd, Shanghai 200040, China;
3. Department of Chemical Engineering, Muhammad Nawaz Sharif University of Engineering and Technology, Multan, Pakistan;
4. Faculty of Chemical, Metallurgical, and Polymer Engineering, University of Engineering & Technology, Lahore, Pakistan

Received:2022-03-08 Revised:2022-06-12 Online:2023-06-13 Published:2023-04-28
Contact: Yisheng Xu,E-mail:yshxu@ecust.edu.cn
Supported by:
This work was financially supported by the National Key Research and Development Program of the International Scientific and Technological Innovation Cooperation Project among Governments (2021YFE0100400), Science and Technology Innovation Action Plan of Shanghai (22501100500), and the international One Belt One Road Collaboration Project of Shanghai (18490740300). The authors greatly thank Prof. A. Kayitmazer Basak at the Bogazici University for providing helpful discussions in the preparation process of NPs.

Polymeric assembled nanoparticles through kinetic stabilization by confined impingement jets dilution mixer for fluorescence switching imaging

Jingran Liu¹, Yue Wu¹, Jie Tang¹, Tao Wang¹, Feng Ni², Qiumin Wu¹, Xijiao Yang¹, Ayyaz Ahmad³, Naveed Ramzan⁴, Yisheng Xu¹

1. State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China;
2. Shanghai Shyndec Pharmaceutical Co., Ltd, Shanghai 200040, China;
3. Department of Chemical Engineering, Muhammad Nawaz Sharif University of Engineering and Technology, Multan, Pakistan;
4. Faculty of Chemical, Metallurgical, and Polymer Engineering, University of Engineering & Technology, Lahore, Pakistan

通讯作者: Yisheng Xu,E-mail:yshxu@ecust.edu.cn
基金资助:
This work was financially supported by the National Key Research and Development Program of the International Scientific and Technological Innovation Cooperation Project among Governments (2021YFE0100400), Science and Technology Innovation Action Plan of Shanghai (22501100500), and the international One Belt One Road Collaboration Project of Shanghai (18490740300). The authors greatly thank Prof. A. Kayitmazer Basak at the Bogazici University for providing helpful discussions in the preparation process of NPs.

Abstract

Abstract: Traditional fluorescence switching molecules achieving the state change between on and off states commonly based on UV irradiation. However, it is worth noting that UV irradiation is harmful to both the cancer cells and the normal cells. To achieve fluorescence switching under visible wavelength and avoid complicate molecular design, a fluorophore of 2,4,5,6-tetrakis(carbazol-9-yl)-1,3-dicyanobenzene (4CzIPN) and a quencher of diarylethene (DAE) were physically incorporated within the biocompatible block copolymer poly(lactic-co-glycolic acid)-b-poly(ethylene glycol) (PLGA-b-PEG) to form 4CzIPN-DAE nanoparticles (NPs) through flash nanoprecipitation (FNP). By using the FNP method, the NPs were prepared within milliseconds in a confined impingement jets dilution (CIJ-D) mixer. Quenching and recovery of fluorescence could achieve in the presence of DAE under 475 nm and 560 nm irradiation. Appropriate structure and fluorescent properties of the nanoparticles can be tuned by external conditions for their efficient fluorescence resonance energy transfer (FRET) in a kinetic stabilization process. This NPs formation process was further optimized by varying the dilution ratio, Reynolds number (Re) and polymer concentration to modulate the mixing and particle nucleation and growth process. The size and fluorescence switching properties of the NPs were systematically investigated in solution and in cellular uptake experiments. This work is anticipated to provide a simple and highly effective engineering strategy for the modulation of fluorescence switching nanoparticles and beneficial to its engineering application.

Key words: Preparation, Kinetic stabilization, Flash nanoprecipitation, Nanoparticles, Fluorescence switch

摘要： Traditional fluorescence switching molecules achieving the state change between on and off states commonly based on UV irradiation. However, it is worth noting that UV irradiation is harmful to both the cancer cells and the normal cells. To achieve fluorescence switching under visible wavelength and avoid complicate molecular design, a fluorophore of 2,4,5,6-tetrakis(carbazol-9-yl)-1,3-dicyanobenzene (4CzIPN) and a quencher of diarylethene (DAE) were physically incorporated within the biocompatible block copolymer poly(lactic-co-glycolic acid)-b-poly(ethylene glycol) (PLGA-b-PEG) to form 4CzIPN-DAE nanoparticles (NPs) through flash nanoprecipitation (FNP). By using the FNP method, the NPs were prepared within milliseconds in a confined impingement jets dilution (CIJ-D) mixer. Quenching and recovery of fluorescence could achieve in the presence of DAE under 475 nm and 560 nm irradiation. Appropriate structure and fluorescent properties of the nanoparticles can be tuned by external conditions for their efficient fluorescence resonance energy transfer (FRET) in a kinetic stabilization process. This NPs formation process was further optimized by varying the dilution ratio, Reynolds number (Re) and polymer concentration to modulate the mixing and particle nucleation and growth process. The size and fluorescence switching properties of the NPs were systematically investigated in solution and in cellular uptake experiments. This work is anticipated to provide a simple and highly effective engineering strategy for the modulation of fluorescence switching nanoparticles and beneficial to its engineering application.

关键词: Preparation, Kinetic stabilization, Flash nanoprecipitation, Nanoparticles, Fluorescence switch

Jingran Liu, Yue Wu, Jie Tang, Tao Wang, Feng Ni, Qiumin Wu, Xijiao Yang, Ayyaz Ahmad, Naveed Ramzan, Yisheng Xu. Polymeric assembled nanoparticles through kinetic stabilization by confined impingement jets dilution mixer for fluorescence switching imaging[J]. Chinese Journal of Chemical Engineering, 2023, 56(4): 89-96.

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Polymeric assembled nanoparticles through kinetic stabilization by confined impingement jets dilution mixer for fluorescence switching imaging

Polymeric assembled nanoparticles through kinetic stabilization by confined impingement jets dilution mixer for fluorescence switching imaging

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